한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제40권7호
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  • Pages.975-980
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  • 2011
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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반응표면분석법에 의한 1-Palmitoyl-2-Oleoyl-3-Oleoyl Glycerol과 1-Palmitoyl-2-Oleoyl-3-Palmitoyl Glycerol의 아세톤 분별 공정 최적화

Optimization of Acetone-Fractionation for 1-Palmitoyl-2-Oleoyl-3-Oleoyl Glycerol and 1-Palmitoyl-2-Oleoyl-3-Palmitoyl Glycerol by Response Surface Methodology

  • Shin, Jung-Ah (Dept. of Food Science and Technology, Chungnam National University) ;
  • Sung, Min-Hye (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Sun-Mo (Dept. of Food Science and Technology, Chungnam National University) ;
  • Son, Jeoung-Mae (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Jeung-Hee (Dept. of Food Science and Technology, Chungnam National University) ;
  • Hong, Soon-Taek (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • 투고 : 2011.05.20
  • 심사 : 2011.06.28
  • 발행 : 2011.07.31
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초록

팜 스테아린으로부터 분별온도($X_1$=25, 30, $35^{\circ}C$)와 팜 스테아린에 대한 아세톤의 비율($X_2$=1:3, 1:6, 1:9, w/v)을 요인 변수로 하여 중심합성계획에 의한 반응표면분석을 이용하여 최적화한 POP purity($Y_1$, %), POO purity($Y_2$, %), POO+POP의 content($Y_3$, g)의 조건을 살펴보았다. $Y_1$, $Y_2$, $Y_3$의 종속요인들을 모두 최대화하면서 순도를 가장 높일 수 있는 분별조건을 예측한 결과, 분별온도 $29.3^{\circ}C$에서 팜 스테아린에 대한 사용 아세톤의 비율이 1:5.7일 경우 60.9%의 POP와 23.8%의 POO를 함유한 POO와 POP 분별유를 4 g의 팜 스테아린에서 3.0 g(75% 수율)의 획득을 예측할 수 있었다. POO와 POP의 순도를 높이면서 함량을 높이는 최적화 조건의 검증을 위하여 반복실험을 수행한 결과, POP purity와 POO purity, POO+POP의 content는 각각 60.18%, 24.10%, 74.38%(2.97 g)로, 반복 실험 간에 유의적인 차이가 없음을 확인하였다. 이렇게 얻어진 POO와 POP 함량 및 순도가 높은 유지는 앞으로 CBE의 주된 대칭형 TAG(POS와 SOS) 합성에 이용될 수 있을 것으로 사료된다.

1-Palmitoyl-2-oleoyl-3-oleoyl glycerol (POO) and 1-palmitoyl-2-oleoyl-3-palmitoyl glycerol (POP) were enriched from palm stearin using an acetone fractionation process. Response surface methodology was employed to optimize the purity of POO ($Y_1$, %) and POP ($Y_2$, %) along with POO+POP content ($Y_3$, g) based on independent variables such as fractionation temperature ($X_1$, 25, 30, and $35^{\circ}C$) and the ratio of palm stearin to acetone ($X_2$, 1:3, 1:6 and 1:9, w/v). Fractionation conditions were optimized to maximize $Y_1$, $Y_2$, and $Y_3$, in which fractionation temperature was $29.3^{\circ}C$ with a 1:5.7 acetone ratio. With such parameters, 60.9% of POP and 23.8% of POO purity were expected with a 75% yield (3.0 g) of POO and POP.

키워드

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피인용 문헌

  1. Comparison of Solid Fat Index and Triacylglycerol Composition of the Blends from Natural Vegetable Fats and Palm Stearin Fraction vol.40, pp.10, 2011, https://doi.org/10.3746/jkfn.2011.40.10.1438
  2. Fatty acid composition and triacylglycerol species of the domestic and foreign chocolates collected from the market vol.40, pp.1, 2013, https://doi.org/10.7744/cnujas.2013.40.1.035
  3. Enzymatic reaction model for the production of symmetrical lipid molecules using the response surface methodology vol.45, pp.2, 2018, https://doi.org/10.7744/kjoas.20180023