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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Transduced Tat-Annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells

  • Lee, Sun-Hwa (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Kim, Dae-Won (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Back, Su-Sun (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Hwang, Hyun-Sook (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Park, Eun-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Kang, Tae-Cheon (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Kwon, Oh-Shin (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Jong-Hoon (Department of Biological Sciences, Sookmyung Women's University) ;
  • Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine) ;
  • Han, Kyu-Hyung (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Eum, Won-Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, College of Medicine, Hallym University)
  • Received : 2011.04.12
  • Accepted : 2011.05.30
  • Published : 2011.07.31
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Abstract

Annexin-1 (ANX1) is an anti-inflammatory protein as well as an important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate the protective effects of ANX1 on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced Tat-ANX1 protein markedly inhibited the expression of cyclooxygenase-2, production of prostaglandin $E_2$, and generation of pro-inflammatory cytokines in the cells. Furthermore, transduced Tat-ANX1 protein caused a significant reduction in the activation of nuclear factor-kappa B (NF-${\kappa}B$) and mitogen-activated protein kinase (MAPK). The results indicate that Tat-ANX1 inhibits the production of inflammatory response cytokines and enzymes by blocking NF-${\kappa}B$ and MAPK. Therefore, Tat-ANX1 protein may be useful as a therapeutic agent against various inflammatory diseases.

Keywords

References

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