Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Neuronal Cell Protection and Antioxidant Activities of Hot Water Extract from Commercial Buckwheat Tea

시판 메밀차 열수 추출물의 항산화 및 신경세포 보호효과

  • Jeong, Chang-Ho (Department of Food Science and Biotechnology, Institute of Life Science an Resources, Kyung Hee University) ;
  • Jeong, Hee-Rok (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Shim, Ki-Hwan (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 정창호 (경희대학교 식품공학과) ;
  • 정희록 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 최성길 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 심기환 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 허호진 (경상대학교 식품공학과.농업생명과학연구원)
  • Received : 2010.12.01
  • Accepted : 2011.05.06
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant and neuronal cell-protective effects of hot water extract from commercial buckwheat tea (CBTE) were evaluated. The 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and malondialdehyde (MDA) inhibitory effect of the CBTE increased in a dose-dependent manner. The Intracellular reactive oxygen species (ROS) accumulation that resulted from hydrogen peroxide ($H_2O_2$) treatment more significantly decreased when CBTE was present in the media than when the PC12 cells were treated only with $H_2O_2$. In the neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), the aqueous extracts showed a protective effect against $H_2O_2$-induced neurotoxicity, and the lactate dehydrogenase (LDH) release into the medium was also inhibited by CBTE. The total phenolics of CBTE was 9,608.10 mg/100 g, and the major phenolic compounds were rutin (13.42 mg/100 g) and quercitrin (0.90 mg/100 g). These data suggested that CBTE, including the aforementioned phenolics, may be useful in reducing the risk of neurodegenerative disease.

본 연구에서는 시판 메밀차 열수 추출물의 항산화 효과 및 신경세포 보호효과를 조사하였다. 시판 메밀차 열수 추출물의 ABTS 라디칼 소거 활성, FRAP 및 MDA 생성 저해 실험결과 농도 의존적인 경향이 나타났으며 또한 높은 항산화 활성을 보여주었다. 과산화수소로 유발된 산화적 손상에 의한 ROS 축적량을 조사한 결과 $H_2O_2$ 단독 처리구보다 메밀차 열수 추출물 처리구에서 낮은 ROS 축적량을 나타내었다. MTT 및 LDH 분석을 통한 PC12 세포 중의 신경세포 보호효과를 측정한 결과 MTT 분석에서는 시판 메밀차 열수 추출물의 모든 농도에서 높은 세포 생존율을 나타냈고, LDH 분석에서는 추출물에 의한 농도 의존적인 세포질 효소 (LDH) 방출량 감소가 관찰되었다. 총 페놀성 화합물, rutin 및 quercitrin의 함량은 각각 9,608.10 mg/g, 13.42 및 0.90 mg/100 g이었다. 본 연구결과를 종합해 볼 때 rutin 및 quercitrin과 같은 다양한 페놀성 화합물을 함유한 시판 메밀차 추출물은 항산화 활성과 산화적 스트레스로 유발된 신경세포 보호효과를 나타내어 퇴행성 신경질환 등을 예방 할 수 있는 기능성 식품 소재로서의 활용 가치가 높을 것으로 판단된다.

Keywords

References

  1. Yoon MY, Kim JY, Hwang JH, Cha MR, Lee MR, Jo KJ, Park HR (2007) Protective effect of methanolic extracts from Dendrobium nobile Lindle. on $H_{2}O_{2}$-induced neurotoxicity in PC12 cells. J Korean Soc Apple Biol Chem, 50, 63-67
  2. Sagara Y, Dargusch R, Chambers D, Davis J, Schubert D, Mater P (1998) Cellular mechanisms of resistance to chronic oxidative stress. Free Radic Biol Med, 24, 1375-1389 https://doi.org/10.1016/S0891-5849(97)00457-7
  3. Lee YS (2007) Antioxidative and physiological activity of extracts of Angelica dahurica leaves. Korean J Food Preserv, 14, 78-86
  4. Kim DJ, Seong KS, Kim DW, Ko SR, Chang CC (2004) Antioxidative effects of red ginseng saponins on paraquat-induced oxidative stress. J Ginseng Res, 28, 5-10 https://doi.org/10.5142/JGR.2004.28.1.005
  5. Ames BN (1983) Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science, 221, 1256-1264 https://doi.org/10.1126/science.6351251
  6. Ames BN, Shigenaga MK, Hagen TM (1993) Oxidant, antioxidants, and the degenerative disease of aging. Proc Natl Acad Sci, USA, 90, 7915-7922 https://doi.org/10.1073/pnas.90.17.7915
  7. Heo HJ, Choi SJ, Choi SG, Shin DH, Lee JM, Lee CY (2008) Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 Cells. J Food Sci, 73, 28-32 https://doi.org/10.1111/j.1750-3841.2007.00632.x
  8. Eum HS, Kang EB, Lim YH, Lee JR, Cho IH, Kim YS, Chae KR, Hwang DY, Kwak YS, Oh YS, Cho JY (2008) The effect of exercise training on A$\beta$-42, BDNF, GLUT-1 and HSP-70 proteins in a NSE/ APPsw- transgenic model for Alzheimer's disease. J Life Sci, 18, 796-803 https://doi.org/10.5352/JLS.2008.18.6.796
  9. Yoon MY, Lee BB, Kim JY, Kim YS, Park EJ, Lee SC, Park HR (2007) Antioxidant activity and neuroprotective effect of Psoralea corylifolia Linne Extracts. Kor J Pharmacogn, 38, 84-89
  10. Kim YS, Chung SH, Suh HJ (1994) Rutin and mineral contents on improved kinds of Korean buckwheat at growing stage. Korean J Food Sci Technol, 26, 759-763
  11. Yeshajahu P, George SR (1972) Amino acid composition of buckwheat. J Agric Food Chem, 20, 270-274 https://doi.org/10.1021/jf60180a029
  12. Havsteen B (1983) Flavonoids a class of natural products of high pharmaco-logical potency. Biochem Pharm, 32, 1141-1148 https://doi.org/10.1016/0006-2952(83)90262-9
  13. Lee JS, Son HS, Maeng YS, Chang YK, Ju JS (1994) Effects of buchwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J Nutr, 27, 819-827
  14. Choi SN, Chung NY (2007) The quality characteristics of bread with added buckwheat powder. Korean J Food Cookery Sci, 23, 664-670
  15. Lee JS, Lee MH, Son HS, Mang YS (1996) Effects of buchwheat on the activities of pancreatic digestive enzymes in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 25, 831-838
  16. Zhang M, Chen H, Li J, Pei Y, Liang Y (2010) Antioxidant properties of tartary buckwheat extracts as affected by different thermal processing methods. LWT-Food Sci Technol, 43, 181-185 https://doi.org/10.1016/j.lwt.2009.06.020
  17. Li CH, Matsui T, Matsumoto K, Yamasaki R, Kawasaki T (2002) Latent production of angiotensin I-converting enzyme inhibitors from buckwheat protein. Journal of Peptide Science, 8, 267-274 https://doi.org/10.1002/psc.387
  18. Wei C, Wei-Jun C, Zhi-Rong S, Ya-Ping Y (2008) Protective effects of ethanolic extracts of buckwheat groats on DNA damage caused by hydroxyl radicals. Food Research International, 41, 924-929 https://doi.org/10.1016/j.foodres.2007.10.014
  19. Hwang EJ, Lee SY, Kwon SJ, Park MH, Boo HO (2006) Antioxidative, antimicrobial and cytotoxic activities of Fagopyrum esculentum Möench extract in germinated seeds. Korean J Medicinal Crop Sci, 14, 1-7
  20. Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS (2004) Antioxidative and antimutagenic effects of Korean buchwheat, sorghum, millet and job's tears. J Korean Soc Food Sci Nutr, 33, 921-929 https://doi.org/10.3746/jkfn.2004.33.6.921
  21. Suh HJ, Chung SH, Kim YS, Lee SD (1997) Free radical scavenging activities and inhibitory effects on xanthine oxidase of buckwheat(Suwon No 5). J Korean Soc Food Sci Nutr, 26, 411-416
  22. Lee MH, Lee JS, Yang HC (2008) $\alpha$-amylase inhibitory activity of flower and leaf extracts from buckwheat (Fagopyrum esculentum). J Korean Soc Food Sci Nutr, 37, 42-47 https://doi.org/10.3746/jkfn.2008.37.1.42
  23. Lee YS, Lee JH (2006) Screening of active substance FS11052 as an inhibitor of neurotransmitter release from PC12 cells. Korean J Vet Res, 46, 87-96
  24. Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemical from various cultivars of plums. Food Chem, 81, 321-326 https://doi.org/10.1016/S0308-8146(02)00423-5
  25. Pellegrin N, Re R, Yang M, Rice-Evans C (1998) Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol, 299, 379-389
  26. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem, 239, 70-76 https://doi.org/10.1006/abio.1996.0292
  27. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF (2001). Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem, 49, 3420-3424 https://doi.org/10.1021/jf0100907
  28. Heo HJ, Cho HY, Hong BS, Kim EK, Kim BK, Shin DH (2001) Protective effect of 4',5-dihydroxy-3', 6,7-trimethoxyflavone from Artemisia asiatica against A$\beta$-induced oxidative stress in PC12 cells. Amyloid, 8, 194-201 https://doi.org/10.3109/13506120109007362
  29. Jeong CH, Choi SG, Heo HJ (2008) Analysis of nutritional components and evaluation of functional activities of Sasa borealis leaf tea. Korean J Food Sci Technol, 40, 586-592
  30. Maeng YS, Park HK, Kwon TB (1990) Analysis of rutin contents in buckwheat and buckwheat food. Korean J Food Sci Technol, 22, 732-737
  31. Ya-ping Y, Cheng-rui T, Wei C (2008) Anti-oxidative constituents of ethanol extract from buckwheat seeds by HPLC-electro-spray MS. Agricultural Sciences in China, 7, 356-362 https://doi.org/10.1016/S1671-2927(08)60076-0
  32. Tang CH, Peng J, Zhen DW, Chen Z (2009) Physicochemical and antioxidant properties buckwheat (Fagopyrum esculentum Moench) protein hydrolysates. Food Chem, 115, 672-678 https://doi.org/10.1016/j.foodchem.2008.12.068
  33. Osawa T (1994) Novel natural antioxidant for utilization in food and biological system. pp. 241-251. In : Postharvest biochemistry of plant food-material in the tropics. Uritani I, Garcia VV, Mendoza EM (ed). Japan Scientific Societies Press, Tokyo, Japan
  34. Uchida K, Stadtman ER (1993) Covalent attachment of 4-hydroxynonenal to glyceraldehyde-3-phosphate dehydrogenese. A possible involvement of intra and intermolecular cross-linking reaction. J Biol Chem, 268, 6388-6393
  35. Colditz GA, Branch LG, Lipnick RJ, Willett WC, Rosner B, Posner BM, Hennekens CH (1985) Increased green and yellow vegetable intake and lowered cancer deaths in an elderly population. Am J Clin Nutr, 41, 32-36 https://doi.org/10.1093/ajcn/41.1.32
  36. Choi SJ, Yoon KY, Choi SG, Kim DO, Oh SJ, Jun WJ, Shin DH, Cho SH, Heo HJ (2007) Protective effect of Acanthopanax senticosus on oxidative stress induced PC12 cell death. Food Sci Biotechnol, 16, 1035-1040
  37. Jeong CH, Kwak JH, Kim JH, Choi GN, Jeong HR, Heo HJ (2010) Neuronal cell protective effects of methanol extract from Cheonggukjang using in vitro system. Korean J Food Sci Technol, 42, 768-772
  38. Jeong CH, Kwak JH, Kim JH, Choi GN, Kim DO, Heo HJ (2010) Neuronal cell protective and antioxidant effects of phenolics obtained from Zanthoxylum piperitum leaf using in vitro model system. Food Chem, 125, 417-422

Cited by

  1. Neuroprotective activities of fermented Ganoderma lucidum extracts by lactic acid bacteria against H2O2-stimulated oxidative stress in PC12 cells vol.24, pp.4, 2015, https://doi.org/10.1007/s10068-015-0181-1
  2. Characteristics of whole buckwheat by wet grinding over time vol.21, pp.2, 2014, https://doi.org/10.11002/kjfp.2014.21.2.181
  3. Antioxidant and Anti-Adipogenic Effects of Ethanolic Extracts from Tartary and Common Buckwheats vol.19, pp.1, 2012, https://doi.org/10.11002/kjfp.2012.19.1.123
  4. Discriminability of Molecular Markers Based on Muclear Ribosomal ITS Sequences of Fagopyrum esculentum and F. tataricum vol.26, pp.4, 2018, https://doi.org/10.11625/KJOA.2018.26.4.745
  5. Antioxidant Activities of Aqueous Extracts from Three Cultivars of Guava Leaf vol.21, pp.6, 2012, https://doi.org/10.1007/s10068-012-0207-x
  6. 잡곡발효물의 제조와 항산화 활성 비교 vol.42, pp.8, 2011, https://doi.org/10.3746/jkfn.2013.42.8.1175
  7. 메밀분말을 첨가한 닭고기 소시지의 품질 특성 vol.44, pp.2, 2011, https://doi.org/10.5536/kjps.2017.44.2.135
  8. 일반메밀과 쓴메밀 종실 추출물의 RAW 264.7 대식세포에서 LPS에 의해 유도되는 iNOS 및 염증성 사이토카인 발현 저해를 통한 항염증 효과 비교 vol.51, pp.6, 2019, https://doi.org/10.9721/kjfst.2019.51.6.565