Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Alcohol Detoxification Beverage that Contained Bulnesia sarmienti on Alcohol-metabolizing Enzymes and Antioxidant Enzyme Activities

Bulnesia sarmienti 를 함유한 숙취해소 음료가 알코올대사 및 항산화 효소활성에 미치는 영향

  • 임애경 (대구테크노파크 바이오산업지원센터) ;
  • 정미정 (부경대학교 식품영양학과) ;
  • 이재욱 (대구테크노파크 바이오산업지원센터) ;
  • 홍주헌 (대구가톨릭대학교 외식식품산업학부) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 정석방 ((주)럭키약품) ;
  • 김대익 (대구테크노파크 바이오산업지원센터)
  • Received : 2010.11.26
  • Accepted : 2011.05.06
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the effect of a beverage that contained Bulnesia sarmienti(BSP, 2.5%) on rats to which alcohol was administered. The treatment of the BSP group reduced the serum alcohol concentration to 52%, compared to 47% in the positive control(PC) group. Similar pattell1s were observed in the enhancement of alcohol dehydrogenase(ADH), acetaldehyde dehydrogenase(ALDH), alkaline phosphate(ALP), alanine aminotransferase(ALT), asparate aminotransferase(AST), total cholesterol(CHOL), ${\gamma}$-glutamyltrasferase(GGT), glucose(GLU), total bilirubin, and total protein(TP) in the serum. Also, in the BSP group, the lipoxidase(LPO), glutathion-S-transferase(GST), XO, catalase(CAT), and superoxide dismutase(SOD) were significantly reduced, compared to the CO and PC groups in the liver. The glutathione(GSH) activity increased in the BSP group, though. These results indicate that Bulnesia sarmienti extract can enhance alcohol metabolization activity.

허브의 일종인 B. sarmienti를 이용하여 숙취 해소 음료를 개발하고 그에 따른 알콜 대사 및 항산화 효소에 미치는 영향을 알아보고자 하였다. 알코올을 섭취한 쥐에 B. sarmienti 음료군(BSP)을 투여 한 경우 타사 제품군인 PC 그리고 B. sarmienti만을 제외한 음료군인 NBS보다 더 뛰어난 알코올 분해능 및 ADH, ALDH에서도 뛰어난 활성을 보였다. 또한 생화학적 검사를 통해 안전성을 확인하였으며, ALP, ALT 그리고 AST에서도 PC군에 비해 유의적인 감소 효과를 보였다. 항산화 활성 및 효소계에 있어서도 BSP군에서는 GSH에서는 CO군에 비해 효소의 함량은 높으나, 다른 군에 비해 유의적인 차이는 보이지 않았으며, TBARS에는 다른 군에 비해 함량이 감소됨을 알 수 있었으나 뚜렷한 차이는 보이지 않았다. 그러나 GST, CAT 그리고 SOD 효소에 있어서는 CO군에 비해 유의적인 효소 활성이 감소되었음을 알 수 있었다. 이상과 같은 결과를 토대로 B. sarmienti 추출물이 첨가된 음료는 뛰어난 숙취 해소 및 간세포의 손상을 보호하는 음료로서 이용 가능하리라 기대 된다.

Keywords

References

  1. Jo DH, Min KJ, Cha CG (2007) The antioxidant and antitumor effects of the extract of Bulnesia sarmientia. J Food Hyg Safety, 22, 120-126
  2. Kamruzzaman SM, Endale M, Oh WJ, Park SC, Kim KS, Hong JH, Kwak YS, Yun BS, Rhee MH (2010) Inhibitory effects of Bulnesia sarmienti aqueous extract on agonist-induced platelet activation and thrombus formation involves mitogen-activated protein kinases. J Ethnopharm, 130, 614-620 https://doi.org/10.1016/j.jep.2010.05.049
  3. Mollah ML, Kim JO, Lee GD, Park CH, Hong JH, Kim HY, Kim KS (2009) Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 cells in vitro and S180 cells in vivo. Immunopharm Immunot, 31, 492-498 https://doi.org/10.1080/08923970902810432
  4. Ottesen AR, Duke JA, Mary JB (2008) Duke's Handbook of Meidicnal Plants of Latin America, CRC press Tylor & Francis Groups, England, p 38-55
  5. Lin RC, Li TK (1998) Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behavior in rats. Amer J Clin Nutr, 68, 1512s-1515s https://doi.org/10.1093/ajcn/68.6.1512S
  6. Hawkins RD, Kalant H (1972) The metabolism of ehtanol and its metabolic effects. Pharmacol Res, 24, 67-157
  7. Forsander OA, Raiha Niels CR (1960) Metabolites produced in the liver during alcohol oxidation. J Biol Chem, 235, 34-36
  8. Wheeler MD, Nakagami M, Bradford BU, Uesugi Y, Mason RP, Connor HD, Dikalova A, Kadiiska M, Thurman RG (2001) Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J Biol Chem, 276, 36664-36672 https://doi.org/10.1074/jbc.M105352200
  9. Abdellah M, Demelliers C, Amsellem S, Pessayre D, Fromenty B (2001) Acute ethanol administration oxidatively damages and depletes mitochondrial DNA in mouse liver, brain, Heart, and skeletal muscles: protective effects of antioxidants. J Pharmacol Exp Ther, 298, 737-743
  10. Kim JS (2004) Effect of a alcohol detoxification beverage(ADB) contained Radix puerariae and Bambusae caules in Liquamen Phyllostachyos on the alcohol administered mouse. J Korean Soc Food Sci Nutr, 33, 318-323 https://doi.org/10.3746/jkfn.2004.33.2.318
  11. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxidase in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  12. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys, 82, 70-77 https://doi.org/10.1016/0003-9861(59)90090-6
  13. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 7130-7139
  14. Della corte E, Stirpe F (1979) The regulation of xanthine oxidase. Biochem J, 117, 97-100
  15. Martin SK, Oduola AM, Milhous WK (1987) Reversal of chloroquine resistance in Plasmodium falciparum by verapamil. Science, 235, 899-901 https://doi.org/10.1126/science.3544220
  16. Aebi H (1984) Catalase. In: L. Packer(Ed), methods in enzymology, Academic pres, Orlando, 105, 121-126
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RS (1951) Protein measurement with the Folin Phenol Reagent. J Biol Chem, 193, 265-275
  18. Cho SH, Kim JC, Kim SW (2001) Effect of plant extracts on the activity of alcohol dehydrogenase and the antioxidation in alcohol-treated rat hepatocyte. J Korean Soc Food Sci Nutr, 30, 679-683
  19. Johnson WJ, Hagge WW, Wagoner RD, Dinapoli RP, Rosevear JW (1972) Effects of urea loading in patients with far-advanced renal failure. Mayo Clin Proc Jan, 47, 21-29

Cited by

  1. Effect of Beverage Containing Fermented Akebia quinata Extracts on Alcoholic Hangover vol.21, pp.1, 2016, https://doi.org/10.3746/pnf.2016.21.1.9
  2. Effects of Polygoni Multiflori Radix on prevention of hyperlipidemia and liver damage induced by alcohol vol.30, pp.3, 2015, https://doi.org/10.6116/kjh.2015.30.3.77.