KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines

염증세포주를 이용한 생체재료 용출물의 체외 생체적합성 평가

  • 신연호 (중앙대학교 병리학교실) ;
  • 송계용 (중앙대학교 병리학교실) ;
  • 서민지 (동국대학교 의생명공학과) ;
  • 김성민 (동국대학교 의생명공학과) ;
  • 박정극 (동국대학교 의생명공학과) ;
  • 김동섭 (한국식품의약품안전청) ;
  • 박기정 (한국식품의약품안전청) ;
  • 허찬희 (한국식품의약품안전청) ;
  • 차지훈 (한국식품의약품안전청) ;
  • 서영권 (동국대학교 생명과학연구원)
  • Received : 2011.05.24
  • Accepted : 2011.06.20
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Various biometerials have been researched and have been developed for treatment of some disease through transplantation to body. They have been evaluated by in vitro cytotoxicity test using some skin-derived cell lines for prediction of their biocompatibility in vivo. However, the results of experiments using mesenchymal or epithelial cells could not be considered in vivo immune reaction. In this study, we evaluated the biomaterial-elution (elute from high density polyethylene film) using some cell lines (L929, Jurkat, U937) in vitro, and then that results were compared with in vivo results from guinea pig sensitization test. In sensitization test, saline and elution of syringe could not induce erythema, but only DNCB (hypersensitive chemical) induce erythema at guinea pig sensitization test. In cell experiment, the cytotoxicity results of inflammatory cells (Jurkat; T lymphocyte, U937; monocyte) was no difference with L929 (fibroblast) in the overall trend. However, inflammatory cell lines were only secreted inflammatory cytokine (TNF-${\alpha}$, INF-${\gamma}$) in some materials (biomateriallution, FAC, DNCB). And the biomaterial-elution did not have toxicity to the cells, but it induced the inflammatory cytokines in inflammatory cell lines only. So, we were predicted inflammatory reaction through the cytokine resultes of inflammatory cell lines, and it was more correlated with in vivo results than cytotoxicity test. Therefore, we suggested that the inflammatory cytokine assay using inflammatory cell lines are more effective method in vitro for evaluation of biocompatibility of biomaterials or chemicals.

Keywords

References

  1. Wikipedia, the free encyclopedia. http://en.wikipedia.org/wiki/Allergy.(2011).
  2. Kimber, I., J. S. Pichowski, and C. J. Betts, et al. (2001) Alternative approaches to the identification and characterization of chemical allergens. Toxicol. In Vitro 15: 307-312. https://doi.org/10.1016/S0887-2333(01)00027-3
  3. Steiling, W., D. Basketter, and K. Berthold, et al. (2001) Skin sensitisation testing-new perspectives and recommendations. Food Chern. Toxicol. 39: 293-301. https://doi.org/10.1016/S0278-6915(00)00147-2
  4. Kay, A. B. (2000) Overview of 'allergy and allergic diseases: with a view to the future'. Br. Med. Bull. 56: 843-864. https://doi.org/10.1258/0007142001903481
  5. Cavani, A., C. Albanesi, and C. Traidl, et al. (2001) Effector and regulatory T cells in allergic contact dermatitis. Trends Immunol. 22: 118-120. https://doi.org/10.1016/S1471-4906(00)01815-9
  6. Buehler, E. V. (1965) Delayed Contact Hypersensitivity in the Guinea Pig. Arch. Dermatol. 91: 171-177. https://doi.org/10.1001/archderm.1965.01600080079017
  7. Magnusson, B. and A. M. Kligman (1970) Allergic contact dermatitis in the guinea pig. Illinois: Charles C. Thomas. Springfield.
  8. Gad, S. C., B. J. Dunn, and D. W. Dobbs, et al. (1986) Development and validation of an alternative dermal sensitization test: the mouse ear swelling test (MEST). Toxieol. Appl. Pharmacol. 84: 93-114. https://doi.org/10.1016/0041-008X(86)90419-9
  9. Kimber, I., R. J. Dearman, and E. W. Scholes, et al. (1994) The local lymph node assay: developments and applications. Toxicology 93: 13-31. https://doi.org/10.1016/0300-483X(94)90193-7
  10. GECD guideline for testing of chemicals -skin sensitisation. 1992.
  11. Sandoe, P., S. B. Christiansen, and M. C. Appleby (2003) Farm animal welfare: the interaction of ethical questions and animal welfare science. Animal Welfare 12: 10.
  12. Collins, F. S., G. M. Gray, and J. R. Bucher (2008) Toxicology. Transforming environmental health protection. Science 319: 906-907. https://doi.org/10.1126/science.1154619
  13. Martin, S. F., P. R. Esser, and S. Schmucker, et al. (2010) T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays. Cell. Mol. Life Sci. 67: 4171-4184. https://doi.org/10.1007/s00018-010-0495-3
  14. Krasteva, M., J. Peguet-Navarro, and C. Moulon, et al. (1996) In vitro primary sensitization of hapten-specific T cells by cultured human epidermal Langerhans S. F. Martin et al cells - a screening predictive assay for contact sensitizers. Clin. Exp. Allergy 26: 563-570. https://doi.org/10.1111/j.1365-2222.1996.tb00577.x
  15. Rougier, N., G. Redziniak, and D. Mougin, et al. (2000) In vitro evaluation of the sensitization potential of weak contact allergens using Langerhans-like dendritic cells and autologous T cells. Toxicology 145: 73-82. https://doi.org/10.1016/S0300-483X(99)00222-X
  16. Moed, H., M. von Blomberg, and D. P. Bruynzeel, et al. (2005) Improved detection of allergen-specific T-cell responses in allergic contact dermatitis through the addition of 'cytokine cocktails'. Exp. Dermatol. 14: 634-640. https://doi.org/10.1111/j.0906-6705.2005.00344.x
  17. Song, K. Y., M. K. Kim, and J. G. Chi (2007) Core Pathology. 2nd ed., pp. 45-69. Korea Medical Book, Korea.
  18. Barbara, J. A., X. Van ostade, and A. Lopez (1996) Tumour necrosis factor-alpha (TNF -alpha): the good, the bad and potentially very effective. Immunol. Cell Biol. 74: 434-443. https://doi.org/10.1038/icb.1996.73
  19. Schroder, K., P. J. Hertzog, and T. Ravasi, et al. (2004) Interferongamma: an overview of signals, mechanisms and functions. J. Leukoc. Biol. 75: 163-189.
  20. Masui, T., S. Sakano, and Y Hasegawa, et al. (2005) Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particles. Biomaterials 26: 1695-1702. https://doi.org/10.1016/j.biomaterials.2004.05.017
  21. Bosetti, M., L. Zanardi, and P. Bracco, et al. (2003) In vitro evaluation of the inflammatory activity of ultra-high molecular weight polyethylene. Biomaterials 24: 1419-1426. https://doi.org/10.1016/S0142-9612(02)00526-4
  22. Holding, C. A., D. M. Findlay, and R. Stamenkov, et al. (2006) The correlation of RANK, RANKL and TNFalpha expression with bone loss volume and polyethylene wear debris around hip implants. Biomaterials 27: 5212- 5219. https://doi.org/10.1016/j.biomaterials.2006.05.054
  23. Warashina, H., S. Sakano, and S. Kitamura, et al. (2003) Biological reaction to alumina, zirconia, titanium and polyethylene particles implanted onto murine calvaria. Biomaterials 24: 3655-3661. https://doi.org/10.1016/S0142-9612(03)00120-0