Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Biochemical and Molecular Identification of Antibacterial Lactic Acid Bacteria Isolated from Kimchi

김치에서 항균활성 유산균의 분리 및 동정

  • Kim, Soo-Young (Department of Biological Sciences, Konkuk University) ;
  • Kim, Jong-Doo (Department of Biological Sciences, Konkuk University) ;
  • Son, Ji-Soo (Department of Biological Sciences, Konkuk University) ;
  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Park, Kab-Joo (Research Institute for Basic Sciences, Konkuk University) ;
  • Park, Myeong-Soo (Department of Hotel Culinary Arts, Anyang Science University)
  • 김수영 (건국대학교 생명과학과) ;
  • 김종두 (건국대학교 생명과학과) ;
  • 손지수 (건국대학교 생명과학과) ;
  • 이시경 (건국대학교 응용생명과학부) ;
  • 박갑주 (건국대학교 기초과학연구소) ;
  • 박명수 (안양과학대학 호텔조리과)
  • Received : 2011.04.07
  • Accepted : 2011.06.06
  • Published : 2011.08.31
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Abstract

Total 480 lactic acid-producing bacteria were isolated from five kinds of kimchi, and their antibacterial activity was tested against Salmonella enterica serovar Typhimurium, Bacillus subtilis, and Pseudomonas aeruginosa using an agar diffusion assay. Among them, 340 isolates showed a halo on MRS agar against one or more indicator strains, which were identified using multiplex PCR, an API 50CHL kit, and a 16S rDNA sequence analysis. As a result, 169 Lactobacillus plantarum, 20 Lactobacillus fermentum, two Lactobacillus paracasei ssp. paracasei, two Lactobacillus sp., and 15 Pediococcus sp. were identified. This may be the first report on the isolation of antibacterial Lactobacillus fermentum from kimchi.

항균활성을 나타내는 야생형 유산균을 분리 동정하기 위하여 김치에서 480종의 유산균을 분리하였으며, S. enterica serovar Typhimurium, P. aeruginosa, B. subtilis에 대한 항균활성을 agar diffusion 법을 사용하여 검사하였다. 이들 중 항균활성을 나타내는 균주 340종에 대하여 multiplex PCR과 API 50CHL Kit을 이용한 생화학적 특성검사 및 16S rDNA sequencing을 통해 Lb. plantarum 169균주, Lb. fermentum 20균주, Lb. paracasei spp. paracasei 2균주, Lactobacillus 속 2균주와 Pediococcus 속 15균주를 동정하였다. 이들 균주 중 Lb. fermentum의 경우는 프로바이오틱스로서의 활성이 보고되고 있는 균으로 추가적인 연구가 필요하다고 사료된다.

Keywords

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