Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Changes of Antioxidative Components and Activity of Fermented Tea during Fermentation Period

미생물을 이용한 후발효차의 발효기간별 항산화 성분 및 활성의 변화

  • Kim, Yong-Shik (Dept. of Food and Nutrition, Chungju National University) ;
  • Jo, Cheor-Un (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Choi, Goo-Hee (Dept. of Food and Nutrition, Chungju National University) ;
  • Lee, Kyung-Haeng (Dept. of Food and Nutrition, Chungju National University)
  • 김용식 (충주대학교 식품영양학과) ;
  • 조철훈 (충남대학교 동물자원생명과학과) ;
  • 최구희 (충주대학교 식품영양학과) ;
  • 이경행 (충주대학교 식품영양학과)
  • Received : 2011.05.11
  • Accepted : 2011.07.27
  • Published : 2011.08.31
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Abstract

Changes of antioxidative components and activity of fermented tea manufactured by Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus bulgaricus were evaluated during the fermentation period. The ascorbic acid content in the fermented tea was relatively lower (43.62~62.84 mg%) than that of green tea (66.74 mg%) during the entire fermentation period. The tea fermented by L. bulgaricus, which had the least contact with air, showed less change in ascorbic acid content. The polyphenol content of green tea was 14.88%, whereas that of fermented tea was 11.54~14.12% and it decreased during the fermentation period. The amount of flavonoids in green tea was 7.78 mg%, whereas that of fermented tea was 4.33~7.88 mg%. DPPH radical scavenging activity and ABTS reducing activity of green tea were 87.47% and 203.22 AEAC mg% (ascorbic acid equivalent antioxidant capacity), respectively, whereas those of fermented tea were lower than green tea. Results indicated that the antioxidative components and activity of fermented tea were lower than those of green tea during the fermentation period. But, when the sensory and hygienic quality are considered, fermented tea can be one of the higher quality tea products on the market.

위생적으로 안전성이 확보된 후발효차를 제조하기 위해 Bacillus subtilis, Saccharomyces cerevisiae 및 Lactobacillus bulgaricus를 이용하여 후발효차를 제조한 후 발효시간에 따른 항산화 성분 및 활성의 변화를 측정하였다. 후발효차의 ascorbic acid의 함량은 발효기간 내내 균종에 관계없이 녹차(66.74 mg%)에 비하여 비교적 낮은 함량(43.62~62.84 mg%)을 보였고 산소와의 접촉을 최소화한 L. bulgaricus 균에 의한 발효가 비교적 적은 변화를 보였다. Polyphenol 화합물의 함량은 녹차의 경우 14.88%였으며 후발효차는 11.54~14.12%로 세 균주 모두 발효가 진행됨에 따라 함량이 다소 감소하였다. 실험에 사용한 녹차의 flavonoid 함량은 7.78 mg%였으며 미생물을 이용한 후발효차의 경우에는 4.33~7.88 mg%로 대부분 녹차에서의 함량보다 적은 것으로 나타났다. 항산화 활성의 경우, 녹차의 DPPH 전자공여능 및 ABTS 항산화 활성은 각각 87.47% 및 203.22 AEAC mg%였으나 발효기간에 따른 후발효차에서는 녹차에 비하여는 낮은 활성을 보여 후발효차 제조 시 어느 정도 항산화 성분의 함량 및 활성이 다소 감소하게 되지만 미생물을 이용하여 후발효차를 제조 시 위생적이고 기호도가 우수한 제품을 생산할 수 있는 것으로 판단되었다.

Keywords

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