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A Study of the Enzymatic Interesterification Reaction for Producing Palmitoyl-Oleoyl-Stearoyl Triacylglycerol from the Palm Mid Fraction

팜중부유를 이용한 대칭형 Palmitoyl-Oleoyl-Stearoyl Triacylglycerol의 효소적 합성 반응 연구

  • Lee, Seon-Mo (Dept. of Food Science and Technology, Chungnam National University) ;
  • Shin, Jung-Ah (Dept. of Food Science and Technology, Chungnam National University) ;
  • Hong, Soon-Tack (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • Received : 2011.05.20
  • Accepted : 2011.07.01
  • Published : 2011.08.31

Abstract

Enzymatic interesterification was conducted with the palm mid fraction (PMF) and stearic ethyl ester for 1, 5, and 9 hr at 46$^{\circ}C$. The reaction was catalyzed by Lipozyme TLIM (2, 3, and 4% by weight of total substrates) in a shaking water bath at 180 rpm. As the reaction continued, oleic acid (C18:1) content at the sn-2 position decreased, whereas saturated fatty acid (C16:0 and C18:0) content increased. In the high performance chromatography analysis, 1,3-dipalmitoyl-2-oleoyl glycerol content decreased, whereas 1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl glycerol (POS) content increased up to the reaction equilibrium. The rate of acyl migration increased with increasing molar ratio and enzyme load as well as reaction time. The optimal reaction conditions for maximizing POS content (53.5 area%) and minimizing acyl migration (23.1 area%) were obtained with a PMF : stearic ethyl ester=1:2 (molar ratio), Lipozyme TLIM 3 wt%, and a reaction time of 5 hr.

본 연구에서는 palm mid fraction(PMF)와 stearic ethyl ester를 기질로 Lipozyme TLIM을 이용한 에스테르 교환반응을 실시하였으며 기질의 몰 비율과 효소양 및 시간에 따른 TAG 조성 변화와 함께 POS 구성율 및 acyl migration 정도를 알아보았다. sn-2 위치에서의 주요 지방산 조성 변화는 몰 비율과 효소양, 반응시간이 증가할수록 C18:1은 점차 감소하였고, C16:0과 C18:0은 증가함을 알 수 있었다. 이와 같은 변화는 특히 반응시간이 증가함에 따라 두드러지게 나타났으며 acyl migration 생성과 관계가 깊은 것으로 예측되었다. 그 결과 총 포화지방산의 함량은 최고 37.4 area%까지 증가한 수치를 보였다. 반응 합성물의 주요 TAG 조성으로는 POP, POS, SOS와 같은 대칭형 구조의 TAG가 확인되었으며 반응이 진행됨에 따라 POP는 감소하고 POS가 증가하는 경향을 보였다. Acyl migration과 POS 함량을 고려하였을 때 몰 비율 1:2, 효소양 3 wt%, 5시간 반응 조건에서 POS는 53.5 area%의 높은 함량을 보였으며 이때 총 포화지방산함량은 반응 전 11.9 area%와 비교하여 약 2배 증가한 23.1 area%를 나타내었다. 한편, 전체 반응 조건 중 가장 높은 POS 함량은 55.4 area%의 수치를 보였는데, 이후 몰 비율과 효소양의 증가와 더불어 반응시간이 지속되었음에도 불구하고 POS는 오히려 감소하는 것을 확인할 수 있었다. 또한 반응이 진행되면서 acyl migration에 의해 sn-2 위치에 palmitic acid와 stearic acid가 전이된 PPS, PSS, SSS와 같은 TAG의 생성도 증가함을 알 수 있었다.

Keywords

References

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