Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea

  • Park, Hong-Su (College of Veterinary Medicine, Kyungpook National University) ;
  • Lim, Il-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Sang-Kyu (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Toh-Kyung (Gyeongnam Livestock Promotion Institute) ;
  • Yeo, Sang-Geon (College of Veterinary Medicine, Kyungpook National University)
  • Received : 2011.07.27
  • Accepted : 2011.09.28
  • Published : 2011.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at $50^{\circ}C$ for 30 min, but labile to 100% ethanol, heat at $52{\sim}60^{\circ}C$ for 30 min, 1 M $MgCl_2$ at $50^{\circ}C$ for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Keywords

References

  1. Abe T, Nakamura K, Tojo H, Mase M, Shibahara T, Yamaguchi S, Yuasa N. Histology, immunohistochemistry, and ultrastructure of hydropericardium syndrome in adult broiler breeders and broiler chicks. Avian Dis 1998, 42, 606-612. https://doi.org/10.2307/1592690
  2. Adair BM, Fitzgerald SD. Adenovirus infections; group I adenovirus infections. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE (eds.). Diseases of Poultry. 12th ed. pp. 252-266, Wiley-Blackwell, Ames, 2008.
  3. Adair BM, McFerran JB. Adenoviruses. In: Dufour- Zavala L; American Association of Avian Pathologists (eds.). A Laboratory Manual for the Isolation, Identification, and Characterization of Avian Pathogens. 5th ed. pp. 84-89, American Association of Avian Pathologists, Athens, 2008.
  4. Anjum AD, Sabri MA, Iqbal Z. Hydropericarditis syndrome in broiler chickens in Pakistan. Vet Rec 1989, 124, 247-248. https://doi.org/10.1136/vr.124.10.247
  5. Balamurugan V, Kataria JM, Kataria RS, Verma KC, Nanthakumar T. Characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken. Comp Immunol Microbiol Infect Dis 2002, 25, 139-147. https://doi.org/10.1016/S0147-9571(01)00032-7
  6. Chandra R, Shukla SK, Kumar M, Garg SK. Electron microscopic demonstration of an adenovirus in the hepatocytes of birds experimentally infected with hydropericardium syndrome. Vet Rec 1997, 140, 70-71.
  7. Chu KS, Lee JW, Song HJ. Diagnosis of avian adenovirus-associated hydropericardium hepatitis syndrome. Korean J Vet Serv 2007, 30, 313-319.
  8. Ganesh K, Raghavan R, Gowda RN, Satyanarayana ML, Suryanarayana VV. Purification and characterization of the aetiological agent of hydropericardium hepatitis syndrome from infected liver tissues of broiler chickens. Trop Anim Health Prod 2002, 34, 7-17. https://doi.org/10.1023/A:1013777509538
  9. Hess M. Detection and differentiation of avian adenoviruses: a review. Avian Pathol 2000, 29, 195-206. https://doi.org/10.1080/03079450050045440
  10. Hess M, Raue R, Prusas C. Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium. Avian Pathol 1999, 28, 433-439. https://doi.org/10.1080/03079459994443
  11. Kim JN, Byun SH, Kim MJ, Kim J, Sung HW, Mo IP. Outbreaks of hydropericardium syndrome and molecular characterization of Korean fowl adenoviral isolates. Avian Dis 2008, 52, 526-530. https://doi.org/10.1637/8178-112207-Case
  12. Kim KS, Ha JS, Kim JH. Occurrence of hydropericardium-hepatitis syndrome in broilers and baeksemis raised in Korea. Korean J Vet Res 2007, 47, 285-290.
  13. Kumar R, Chandra R. Studies on structural and immunogenic polypeptides of hydropericardium syndrome virus by SDS-PAGE and western blotting. Comp Immunol Microbiol Infect Dis 2004, 27, 155-161. https://doi.org/10.1016/j.cimid.2003.08.003
  14. MacLachlan NJ, Dubovi EJ. Fenner's Veterinary Virology. 4th ed. pp. 203-212, Academic Press, San Diego, 2010.
  15. Mase M, Mitake H, Inoue T, Imada T. Identification of group I-III avian adenovirus by PCR coupled with direct sequencing of the hexon gene. J Vet Med Sci 2009, 71, 1239-1242. https://doi.org/10.1292/jvms.71.1239
  16. Mazaheri A, Prusas C, Voss M, Hess M. Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens. Avian Pathol 1998, 27, 269-276. https://doi.org/10.1080/03079459808419335
  17. Meulemans G, Boschmans M, van den Berg TP, Decaesstecker M. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol 2001, 30, 655-660. https://doi.org/10.1080/03079450120092143
  18. Meulemans G, Couvreur B, Decaesstecker M, Boschmans M, van den Berg TP. Phylogenetic analysis of fowl adenoviruses. Avian Pathol 2004, 33, 164-170. https://doi.org/10.1080/03079450310001652086
  19. Otsuki K, Tsubokura M, Yamamoto H, Imamura M, Sakagami Y. Some properties of avian adenoviruses isolated from chickens with inclusion body hepatitis in Japan. Avian Dis 1976, 20, 693-705. https://doi.org/10.2307/1589449
  20. Petek M, Felluga B, Zoletto R. Biological properties of CELO virus: stability to various agents, and electronmicroscopic study. Avian Dis 1963, 7, 38-49. https://doi.org/10.2307/1587987
  21. Petek M, Felluga B, Zoletto R, Bersani G. Further studies on CELO virus: Its relationship to the adenovirus group. Arch Virol 1964, 14, 637-649.
  22. Raue R, Hess M. Hexon based PCRs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus. J Virol Methods 1998, 73, 211-217. https://doi.org/10.1016/S0166-0934(98)00065-2
  23. Rovozzo GC, Burke CN. A Manual of Basic Virological Techniques. pp. 126-152, Prentice-Hall, Englewood Cliffs, 1973.
  24. Toro H, Prusas C, Raue R, Cerda L, Geisse C, González C, Hess M. Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile. Avian Dis 1999, 43, 262-270. https://doi.org/10.2307/1592616
  25. Villegas P, Alvarado I. Virus identification and classification. In: Dufour-Zavala L; American Association of Avian Pathologists (eds.). A Laboratory Manual for the Isolation, Identification, and Characterization of Avian Pathogens. 5th ed. pp. 209-216, American Association of Avian Pathologists, Athens, 2008.
  26. Zsak L, Kisary J. Grouping of fowl adenoviruses based upon the restriction patterns of DNA generated by BamHI and HindIII. Intervirology 1984, 22, 110-114. https://doi.org/10.1159/000149541