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Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus

한국호흡기형 닭전염성기관지염 생독백신주의 작성

  • Choi, Kang-Seuk (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Jeon, Woo-Jin (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Eun-Kyoung (Veterinary Medicine and Biologics Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kye, Soo-Jeong (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Park, Mi-Ja (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kwon, Jun-Hun (Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency)
  • 최강석 (농림수산검역검사본부 동식물위생연구부 조류질병과) ;
  • 전우진 (농림수산검역검사본부 동식물위생연구부 조류질병과) ;
  • 이은경 (농림수산검역검사본부 동식물위생연구부 동물약품평가과) ;
  • 계수정 (농림수산검역검사본부 동식물위생연구부 조류질병과) ;
  • 박미자 (농림수산검역검사본부 동식물위생연구부 조류질병과) ;
  • 권준헌 (농림수산검역검사본부 동식물위생연구부 조류질병과)
  • Received : 2011.06.23
  • Accepted : 2011.07.18
  • Published : 2011.09.30

Abstract

An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.

Keywords

References

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