Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Isolation and Antimicrobial Susceptibility of Microorganisms from Milk Samples of Jindo Dogs (Canis familiaris var. jindo)

진도견(Canis familiaris var. jindo) 유즙으로부터 분리한 세균의 분포 및 항균제 감수성

  • Lee, Ju-Dan (Jeollanamdo Livestock Sanitation Office) ;
  • Lee, Youn-Kyong (College of Veterinary Medicine, Chonnam National University) ;
  • O, Suck-Il (Jindo Dog Research and Testing Center) ;
  • Jung, Ji-Young (Jeollanamdo Livestock Sanitation Office) ;
  • Son, Chang-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Sung-Shik (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Ki-Seok (College of Veterinary Medicine, Chonnam National University) ;
  • Hur, Tai-Young (National Institute of Animal Science, RDA) ;
  • Suh, Guk-Hyun (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2011.01.12
  • Published : 2011.03.30
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Abstract

Lack of hygiene and puerperal mastitis are common causes of bacterial diseases in nursing neonates. The aim of this study was to isolate microorganisms from milk samples of healthy female Jindo dogs with suckling puppies and to investigate antimicrobial susceptibility against the isolated bacteria. Milk samples were collected from 120 udders of 12 lactating Jindo dogs that were 2~4 years old without any clinical diseases including mastitis. Bacteria were isolated from 64 milk samples (53.3%), either singly (76.6%) or in combination (23.4%). Staphylococcus (S.) spp. was the most common microorganisms (74.7%) isolated from canine milk, followed by Haemophillus spp. (10.9%), Streptococcus spp. (9.6%), Gardnerella spp. (2.4%) and Moraxella spp. (2.4%). The most frequently isolated organism was S. warneri (31.3%). Antimicrobial susceptibility of these bacteria was tested with 17 antimicrobial agents by Kirbyand Bauer standardized disc diffusion method. Results indicated that bacteria isolated from healthy canine milk were mostly susceptible to amoxicillin-clavulanic acid, cephalothin and ceftiofur, but were resistant to erythromycin, neomycin and tetracycline.

Keywords

References

  1. 강호조, 손원근, 이주홍. 가축유래 Staphylococcus aureus의 enterotoxin 산생과 Plasmid profile에 관한 연구. 한국수의공중보건학회지 1992, 16, 21-26.
  2. 김신, 홍현표, 김상윤, 권헌일, 이희무. 유방염 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출. 한국가축위생학회지 2002, 25, 275-283.
  3. 변정혜, 김태중. 개에서 분리한 Staphylococcus aureus와 S. intermedius의 항생제 감수성. 대한수의학회지 2007, 47, 43-50.
  4. 윤준철, 이정치, 김상기, 박영석, 김종택, 이정길, 이채용. 젖염소 분방 유즙에서 분리한 세균 및 항균제 감수성 조사. 대한수의학회지 2004, 44, 151-157.
  5. 이정치, 이채용, 김상기, 이정길, 서국현. Holstein 유우의 유즙에서 분리한 유방염 원인균의 항균제 감수성. 한국임상학회지 2003, 20, 166-171.
  6. Barigye R, Schaan L, Gibbs PS, Schamber E, Dyer NW. Diagnostic evidence of Staphylococcus warneri as a possible cause of bovine abortion. J Vet Diagn Invest 2007, 19, 694-696. https://doi.org/10.1177/104063870701900613
  7. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1996, 45, 493-496.
  8. Bert F, Lambert-zechovsky N. Septicemia caused by Streptococcus canis in a human. J Clin Microbiol 1997, 35, 777-779.
  9. Cox HU, Hoskins JD, Roy AF, Newman SS, Luther DG. Antimicrobial susceptibility of coagulase-positive staphylococci isolated from Louisiana dogs. Am J Vet Res 1984, 45, 2039-2042.
  10. Espino L, Bermudez R, Fidalgo LE, Gonzalez A, Mino N, Quiroga MI. Meningoencephalitis associated with staphylococcus warneri in a dog. J Small Anim Pract 2006, 47, 598-602. https://doi.org/10.1111/j.1748-5827.2006.00164.x
  11. Ettinger SJ, Feldman EC. Textbook of Veterinary Internal Medicine. 6th ed. pp. 1666-1667, WB Saunders, Philadelpia, 2004.
  12. Hirsh DC, James MacLachlan N, Walker RL. Veterinary Microbiology. 2nd ed. pp. 98, Wiley- Blackwell, Oxford, 2004.
  13. Johnston SD. Management of the postpartum bitch and queen. In: Kirk RW (ed.). Current Veterinary Therapy VIII. pp. 960, WB Saunders, Philadelphia, 1983.
  14. Johnston SD, Root Kustritz MV, Olson PNS. Canine and Feline Theriogenology. pp. 129-138, WB Saunders, Philadelphia, 2001.
  15. Kuhn G, Pohl S, Hingst V. Elevation of the bacteriological content of milk of clinically unaffected lactating bitches of a canine research stock. Berl Munch Tierarztl Wochenschr 1991, 104, 130-133.
  16. Manson JM, Keis S, Smith JMB, Cook GM. Characterization of a vancomycin-resistant Enterococcus faecalis (VREF) isolate from a dog with mastitis: Further evidence of a clonal lineage of VREF in New Zealand. J Clin Microbiol 2003, 41, 3331-3333. https://doi.org/10.1128/JCM.41.7.3331-3333.2003
  17. Munnich A, Lubke-Becker A. Escherichia coli infections in newborn puppies-clinical and epidemiological investigations. Theriogenology 2004, 62, 562-575. https://doi.org/10.1016/j.theriogenology.2003.11.012
  18. Munnich A. The pathological newborn in small animals: the neonate is not a small adult. Vet Res Commun 2008, 32 (Suppl 1), S81-85.
  19. Olson PN, Olson AL. Cytological evaluation of canine milk. Vet Med Small Anim Clin 1984, 79, 641-644.
  20. Schafer-Somi S, Spergser J, Breitenfellner J, Aurich JE. Bacteriological status of canine milk and septicaemia in neonatal puppies-a retrospective study. J Vet Med B Infect Dis Vet Public Health 2003, 50, 343-346. https://doi.org/10.1046/j.1439-0450.2003.00672.x
  21. Silva N. Identification and antimicrobial susceptibility patterns of Staphylococcus spp. isolated from canine chronic otitis externa. Arq Bras Med Vet Zootec 2001, 53, 1-5.
  22. Smith BJ. Canine Anatomy. pp. 13-14, Lippincott Williams & Wilkins, Philadelphia, 1996.
  23. Timoney JF, Gillespie JH, Scott FW, Barlough JE. Hagan and Bruner's Microbiology and Infectious Diseases in Domestic Animals. 8th ed. pp. 92, Cornell University Press, Ithaca, 1988.
  24. Uchida Y, Nakade T, Kitazawa K. Clinicomicrobiological study of the normal and otitic external ear canals in dogs and cats. Nippon Juigaku Zasshi 1990, 52, 415-417. https://doi.org/10.1292/jvms1939.52.415
  25. Ververidis HN, Mavrogianni VS, Fragkou IA, Orfanou DC, Gougoulis DA, Tzivara A, Gouletsou PG, Athanasiou L, Boscos CM, Fthenakis GC. Experimental staphylococcal mastitis in bitches: clinical, bacteriological, cytological, haematological and pathological features. Vet Microbiol 2007, 124, 95-106. https://doi.org/10.1016/j.vetmic.2007.03.029

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