대한수의학회지 (Korean Journal of Veterinary Research)

  • 제51권1호
  • /
  • Pages.15-20
  • /
  • 2011
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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국내 소에서 분리한 Brucella abortus의 병원성 분석

The virulence of Brucella abortus isolated from cattle in Korea

  • 임정주 (경상대학교 수의과대학) ;
  • 김정화 (경상북도 가축위생시험소 북부지소) ;
  • 김동혁 (경상대학교 수의과대학) ;
  • 이진주 (경상대학교 수의과대학) ;
  • 김대근 (경상대학교 수의과대학) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 김상훈 (충남대학교 수의과대학) ;
  • 장홍희 (경상대학교 농업생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학) ;
  • 민원기 (경상대학교 수의과대학) ;
  • 김석 (경상대학교 수의과대학)
  • Lim, Jeong Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jeong-Hwa (Northern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Kim, Dong Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Jin Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dae Geun (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Sang Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Chang, Hong Hee (College of Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hu Jang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
  • 심사 : 2010.12.24
  • 발행 : 2011.03.30
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초록

In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class I, lower internalization than that of B. abortus 544; class II, similar internalization to that of that of B. abortus 544; class III, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class III showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.

키워드

참고문헌

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