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Effects of Environmental Factors and Heavy Metals on the Growth and Phosphorus Removal of Alcaligenes sp.

환경인자와 중금속이 Alcaligenes sp.의 생장과 인 제거에 대한 영향

  • Yoo, Ri-Bi (Department of Environmental Biology and Chemistry, Chungbuk National University) ;
  • Kim, Hee-Jung (Department of Environmental Biology and Chemistry, Chungbuk National University) ;
  • Lee, Seok-Eon (Department of Environmental Biology and Chemistry, Chungbuk National University) ;
  • Lee, Moon-Soon (Department of Industrial Plant, Chungbuk National University) ;
  • Woo, Sun-Hee (Department of Crop Science, Chungbuk National University) ;
  • Choi, Jong-Soon (Proeteomics Team, Korea Basic Science Institute) ;
  • Baek, Ki-Tae (Department of Environmental Engineering, Kumoh National University) ;
  • Chung, Keun-Yook (Department of Environmental Biology and Chemistry, Chungbuk National University)
  • 유리비 (충북대학교 환경생명화학과) ;
  • 김희정 (충북대학교 환경생명화학과) ;
  • 이석언 (충북대학교 환경생명화학과) ;
  • 이문순 (충북대학교 특용식물학과) ;
  • 우선희 (충북대학교 식물자원학과) ;
  • 최종순 (한국 기초과학지원연구원) ;
  • 백기태 (금오공과대학교 환경공학과) ;
  • 정근욱 (충북대학교 환경생명화학과)
  • Received : 2011.05.03
  • Accepted : 2011.06.02
  • Published : 2011.06.30

Abstract

BACKGROUND: This study was performed to evaluate the effects of environmental factors and heavy metals on the growth and phosphorus removal capacity of Alcaligenes sp., which was well known as one of PAOs(Phosphorus Accumulating Microorganisms). METHODS AND RESULTS: The environmental factors used in this study were temperature, pH and carbon sources, and the heavy metals included Cu, Cd, Zn, As, and Ni. The growth and P removal efficiency of Alcaligenes sp. was maximal as temperature, pH, and carbon source were $25^{\circ}C$, 7, and glucose+acetate, respectively. Also, the $IC_{50}$(median inhibitory Concentration) values of Alcaligenes sp. for the Cu, Cd, Zn, As, and Ni were 5.03, 0.08, 0.73, 282.20 and 4.74 mg/L, respectively. CONCLUSION(S): Based on the results obtained from this study, it appears that the growth and P removal efficiency of Alcaligenes sp. were affected by the environment factors and at the best optimum condition for its growth and P removal efficiency, as the concentrations of heavy metals were gradually increased, its growth was correspondingly decreased.

Alcaligenes sp.의 인 제거에 대한 환경인자로 각각 온도, pH, 탄소원의 효과를 알아본 결과 $25^{\circ}C$, pH 7, glucose+acetate이 탄소원일 때 인 제거효율이 가장 좋은 것으로 확인 할 수 있었다. 5가지 중금속의 독성효과에 따른 Alcaligenes sp.의 생장은 중금속의 농도가 높아질수록 억제되었으며, $IC_{50}$값은 구리 5.03 mg/L, 카드뮴 0.08 mg/L, 아연 0.73 mg/L, 비소 282.20 mg/L, 니켈 4.74 mg/L로 나타났다. 모든 중금속의 농도가 높아질수록 생장이 억제되어 인 제거 역시 억제되는 것을 확인 할 수 있었다.

Keywords

References

  1. Bachate, S.P., Cavalca, L., Andreoni, V., 2009. Arsenicresistant bacteria isolated from agricultural soils of Bangladesh and characterization of arsenate-reducing strains, J. of Appl. Microbio. 107(1), 142-156.
  2. Boswell, C.D., Dick, R.E., Macskie, L.E., 1999. The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii, Micrbiol. 145, 1711-1720.
  3. Bundgaard E., G. Petersen, 1991. Methods for improving biological phosphorus removal, Presented at the 1991 WEF Annual Conference, U.S.A.
  4. Choi, J.Y., Lee, J.Y., Yang, J.S., 2009. Biosorption of heavy metals and uranium by starfish and Pseudomonas putida, J. Hazardous Mat. 161, 157-162. https://doi.org/10.1016/j.jhazmat.2008.03.065
  5. Chung, K.Y., Han, S.S., Kim, H.K., Choi, G.S., Kim, I.S., Lee, S.S., Woo, S. H., Lee, K.H., and Kim, J.J., 2006. Inhibitory Effect of the selected heavy metals on the growth of the phosphorus accumulating microorganism, Acinetobacter sp., Korean J. Environ. Agri. 25(1), 40-46. https://doi.org/10.5338/KJEA.2006.25.1.040
  6. Grady, C.P.L., Daigger, G.T., Lim, H.C., 1999. Biological wastewater treatment, 2nd Ed. Marcel Dekker. New York.
  7. Hammaini, F. Gonzalez, A. Ballester, M.L. Blazquez, J.A. Munoz, 2007. Biosorption of heavy metals by activated sludge and their desorption characteristics, J. Envionmen. Manag. 84, 419-426. https://doi.org/10.1016/j.jenvman.2006.06.015
  8. Im, M.S., Son, H.J., Park, S.M., Lee, J.K., Lee, S.J., 1992, Accumulation of poly-$\beta$-hydroxybutyric acid by Alcaligenes sp., Korean J. Appl. Microbiol. Biotechnol. 20(4), 363-370.
  9. Kim, H.J., Yoo, R.B., Han, S.S., Woo, S.H., Lee, M.S., Baek, K.T., Chung., K.Y., 2010. Effect of the various heavy metals on the growth and phosphorus (P) removal capacity of the phosphorus accumulating microorganism (Pseudomonas sp.), Korean J. Environ. Agri. 29(2), 189-196. https://doi.org/10.5338/KJEA.2010.29.2.189
  10. Korstee, G. J. J., Appeldoorn, K. J., Bonting, C. F. C., van Niel, E. W. J., van Veen, H. W., 1994. Biology of polyphosphate-accumulating bacteria involved in enhanced biological phosphorus removal. FEMS Microbiol. Rev. 15(2), 137-153. https://doi.org/10.1111/j.1574-6976.1994.tb00131.x
  11. Levin, G.V. and Sharpiro, J., 1965. Metabolic uptake of phosphorus by wastewater organics. J. Wat. Pollut. Control Fed. 37(6), 800.
  12. Medigan, M.T., Martinko, J.M., Clark, D.P., Dunlap, P.V., 2009. Brock biology of microorganisms, 12th Ed., Person Education, San Francisco.
  13. Mino, T., van Loosdrecht, M.C., and Heijnen, J.J, 1998. Microbiology and biochemistry of the enhanced biological phosphorus removal process, Wat. Res. 32, 3193-3207. https://doi.org/10.1016/S0043-1354(98)00129-8
  14. Panswad, T., Doungchai, A. and Anotai, J., 2003. Temperature effect on microbial community of enhanced biological phosphorus removal system, Water Res. 37(2), 409-415. https://doi.org/10.1016/S0043-1354(02)00286-5
  15. Sedlak, R.I., 1991. Phosphorus and Nitrogen Removal from Municipal Wastewater, 2nd Ed., The Soap and Detergent Association, Lewis Publishers, New York.
  16. Silver Simon, 1996. Bacterial resistances to toxic metal ions: A review, Gene, 179(1), 9-19. https://doi.org/10.1016/S0378-1119(96)00323-X
  17. Wang Y., Ren, Z., Jiang, F., Geng, J., He, W., Yang, J., 2011. Effect of copper ion on the anaerobic and aerobic metabolism of phosphorus accumulating organisms linked to intracellular storage compounds, J. of Hazard. Mat. 186, 313-319. https://doi.org/10.1016/j.jhazmat.2010.11.007
  18. Yan Liu, Yinguang Chen, Qi Zhou, 2007. Effect of initial pH control on enhanced biological phosphorus removal from wastewater containing acetic and propionic acids, Chemosphere, 66(1), 123-129. https://doi.org/10.1016/j.chemosphere.2006.05.004
  19. Yeo, S.M. and Lee, Y.O., 2006. Changes of the Bacterial Community Structure Depending on Carbon Source in Biological Phosphate Removing Process, J. KSEE, 28(2) 165-172.
  20. Zafiri, C, Kornaros, M., Lyberatos, G., 1999. Kinetic modeling of biological phosphorus removal with a pure culture of Acinetobactor sp. under aerobic, anaerobic, and transient operating conditions, Water Res. 33(12), 2769-2788. https://doi.org/10.1016/S0043-1354(98)00522-3

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