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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase

  • Yoon, In-Soo (Department of Advanced Technology Fusion, Konkuk University) ;
  • Chung, Ji-Hyung (Yonsei Integrative Research Institute for Cerebral & Cardiovascular Diseases, Yonsei University Health System) ;
  • Hahm, Soo-Hyun (Department of Advanced Technology Fusion, Konkuk University) ;
  • Park, Min-Ju (Department of Advanced Technology Fusion, Konkuk University) ;
  • Lee, You-Ri (Department of Advanced Technology Fusion, Konkuk University) ;
  • Ko, Sung-Il (Department of Advanced Technology Fusion, Konkuk University) ;
  • Kang, Lin-Woo (Department of Advanced Technology Fusion, Konkuk University) ;
  • Kim, Tae-Sung (Laboratory of Biochemistry, School of Life Sciences and Biotechnology, and BioInstitute, Korea University) ;
  • Kim, Joon (Laboratory of Biochemistry, School of Life Sciences and Biotechnology, and BioInstitute, Korea University) ;
  • Han, Ye-Sun (Department of Advanced Technology Fusion, Konkuk University)
  • Received : 2010.11.12
  • Accepted : 2011.06.13
  • Published : 2011.08.31
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Abstract

Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3.

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References

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