Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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Genetic Variation Analysis of Arabidopsis (Arabidopsis thaliana L.) Plants Induced by Acute and Chronic Gamma Irradiation

감마선 완·급조사에 따른 애기장대의 유전적 유연관계 분석

  • Goh, Eun Jeong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ha, Bo-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 고은정 (한국원자력연구원 방사선육종연구팀) ;
  • 김진백 (한국원자력연구원 방사선육종연구팀) ;
  • 하보근 (한국원자력연구원 방사선육종연구팀) ;
  • 김상훈 (한국원자력연구원 방사선육종연구팀) ;
  • 강시용 (한국원자력연구원 방사선육종연구팀) ;
  • 김동섭 (한국원자력연구원 방사선육종연구팀)
  • Received : 2011.11.07
  • Accepted : 2011.11.30
  • Published : 2011.12.30

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Abstract

In order to identify the genetic relationship analysis by acute and chronic gamma irradiation, Arabidopsis (Arabidopsis thaliana L.) were irradiated with 200 Gy of gamma-rays using gamma-irradiator (3,000 Ci; Nordion, Canada) and gamma-phytotron (400 Ci; Nordion, Canada) for acute and chronic irradiation, respectively. Genetic relationship among two acute gamma-irradiated plants (A1 and A24) and three chronic gamma-irradiated plants (C1W, C2W, C3W) were analyzed using the amplified fragment length polymorphism (AFLP) technique compared with each non-irradiated plant. A total of 28 EcoRI and MseI primer combinations were used to screen 8 treatments by the ABI3130 capillary electrophoresis system. Amplified products by 28 primer sets showed 1,679 bands with an average of 51 bands per primer combination. Out of the total bands scored, 1,164 fragments were polymorphic bands, with different alleles existing among the treatments. The cluster analysis was performed using the UPGMA (Unweighted Pair Group Method using Arithmetic) in the computer program NTSYS-pc. In clustery analysis, acute gamma-irradiation showed higher genetic variation compared with chronic gamma-irradiation.

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