Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Anti-Diabetic Effect of Pectinase-Processed Ginseng Radix (GINST) in High Fat Diet-Fed ICR Mice

  • Yuan, Hai Dan (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Quan, Hai Yan (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Jung, Mi-Song (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Kim, Su-Jung (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Huang, Bo (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Kim, Do-Yeon (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University) ;
  • Chung, Sung-Hyun (Department of Life and Nanopharmaceutical Science, Graduate School of Kyung Hee University)
  • Received : 2011.02.24
  • Accepted : 2011.05.21
  • Published : 2011.09.25
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Abstract

In the present study, we investigate anti-diabetic effect of pectinase-processed ginseng radix (GINST) in high fat diet-fed ICR mice. The ICR mice were divided into three groups: regular diet group, high fat diet control group (HFD), and GINSTtreated group. To induce hyperglycemia, mice were fed a high fat diet for 10 weeks, and mice were administered with 300 mg/kg of GINST once a day for 5 weeks. Oral glucose tolerance test revealed that GINST improved glucose tolerance after glucose challenge. Compared to the HFD control group, fasting blood glucose and insulin levels were decreased by 57.8% (p<0.05) and 30.9% (p<0.01) in GINST-treated group, respectively. With decreased plasma glucose and insulin levels, the insulin resistance index of the GINST-treated group was reduced by 68.1% (p<0.01) compared to the HFD control group. Pancreas of GINST-treated mice preserved a morphological integrity of islets and consequently having more insulin contents. In addition, GINST up-regulated the levels of phosphorylated AMP-activated protein kinase (AMPK) and its target molecule, glucose transporter 4 (GLUT4) protein expression in the skeletal muscle. Our results suggest that GINST ameliorates a hyperglycemia through activation of AMPK/GLUT4 signaling pathway, and has a therapeutic potential for type 2 diabetes.

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References

  1. Liao Z, Chen X, Wu M. Antidiabetic effect of flavones from Cirsium japonicum DC in diabetic rats. Arch Pharm Res 2010;33:353-362. https://doi.org/10.1007/s12272-010-0302-6
  2. Zhu CF, Peng HB, Liu GQ, Zhang F, Li Y. Beneficial effects of oligopeptides from marine salmon skin in a rat model of type 2 diabetes. Nutrition 2010;26:1014-1020. https://doi.org/10.1016/j.nut.2010.01.011
  3. Gabir MM, Hanson RL, Dabelea D, Imperatore G, Roumain J, Bennett PH, Knowler WC. Plasma glucose and prediction of microvascular disease and mortality: evaluation of 1997 American Diabetes Association and 1999 World Health Organization criteria for diagnosis of diabetes. Diabetes Care 2000;23:1113-1118. https://doi.org/10.2337/diacare.23.8.1113
  4. Wang ZQ, Zhang XH, Yu Y, Poulev A, Ribnicky D, Floyd ZE, Cefalu WT. Bioactives from bitter melon enhance insulin signaling and modulate acyl carnitine content in skeletal muscle in high-fat diet-fed mice. J Nutr Biochem 2011; Epub ahead of print.
  5. Abdul-Ghani MA, DeFronzo RA. Pathogenesis of insulin resistance in skeletal muscle. J Biomed Biotechnol 2010;2010:476279.
  6. Shaw RJ, Lamia KA, Vasquez D, Koo SH, Bardeesy N, Depinho RA, Montminy M, Cantley LC. The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin. Science 2005;310:1642- 1646. https://doi.org/10.1126/science.1120781
  7. Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez-Lluch G, Lewis K et al. Resveratrol improves health and survival of mice on a high-calorie diet. Nature 2006;444:337-342. https://doi.org/10.1038/nature05354
  8. Luo JZ, Luo L. Ginseng on hyperglycemia: effects and mechanisms. Evid Based Complement Alternat Med 2009;6:423-427. https://doi.org/10.1093/ecam/nem178
  9. Huang J, Tang XH, Ikejima T, Sun XJ, Wang XB, Xi RG, Wu LJ. A new triterpenoid from Panax ginseng exhibits cytotoxicity through p53 and the caspase signaling pathway in the HepG2 cell line. Arch Pharm Res 2008;31:323-329. https://doi.org/10.1007/s12272-001-1159-8
  10. Park EK, Shin YW, Lee HU, Kim SS, Lee YC, Lee BY, Kim DH. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of RAW264.7 cells induced by lipopolysaccharide. Biol Pharm Bull 2005;28:652-656. https://doi.org/10.1248/bpb.28.652
  11. Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, Okada S. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radic Biol Med 1996;20:145-150. https://doi.org/10.1016/0891-5849(95)02020-9
  12. Chang Y, Huang WJ, Tien LT, Wang SJ. Ginsenosides Rg1 and Rb1 enhance glutamate release through activation of protein kinase A in rat cerebrocortical nerve terminals (synaptosomes). Eur J Pharmacol 2008;578:28-36. https://doi.org/10.1016/j.ejphar.2007.09.023
  13. Han GC, Ko SK, Sung JH, Chung SH. Compound K enhances insulin secretion with beneficial metabolic effects in db/db mice. J Agric Food Chem 2007;55:10641-10648. https://doi.org/10.1021/jf0722598
  14. Lee WK, Kao ST, Liu IM, Cheng JT. Ginsenoside Rh2 is one of the active principles of Panax ginseng root to improve insulin sensitivity in fructose-rich chow-fed rats.Horm Metab Res 2007;39:347-354. https://doi.org/10.1055/s-2007-976537
  15. Park MW, Ha J, Chung SH. 20(S)-ginsenoside Rg3 enhances glucose-stimulated insulin secretion and activates AMPK. Biol Pharm Bull 2008;31:748-751. https://doi.org/10.1248/bpb.31.748
  16. Zhang Z, Li X, Lv W, Yang Y, Gao H, Yang J, Shen Y, Ning G. Ginsenoside Re reduces insulin resistance through inhibition of c-Jun NH2-terminal kinase and nuclear factor-kappaB. Mol Endocrinol 2008;22:186-195. https://doi.org/10.1210/me.2007-0119
  17. Yuan HD, Kim SJ, Chung SH. Beneficial effects of IH- 901 on glucose and lipid metabolisms via activating adenosine monophosphate-activated protein kinase and phosphatidylinositol-3 kinase pathways. Metabolism 2011;60:43-51. https://doi.org/10.1016/j.metabol.2009.12.024
  18. Kusakabe T, Tanioka H, Ebihara K, Hirata M, Miyamoto L, Miyanaga F, Hige H, Aotani D, Fujisawa T, Masuzaki H et al. Beneficial effects of leptin on glycaemic and lipid control in a mouse model of type 2 diabetes with increased adiposity induced by streptozotocin and a highfat diet. Diabetologia 2009;52:675-683. https://doi.org/10.1007/s00125-009-1258-2
  19. Sone H, Kagawa Y. Pancreatic beta cell senescence contributes to the pathogenesis of type 2 diabetes in high-fat diet-induced diabetic mice. Diabetologia 2005;48:58-67. https://doi.org/10.1007/s00125-004-1605-2
  20. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985;28:412-419. https://doi.org/10.1007/BF00280883
  21. Choi K, Kim YB. Molecular mechanism of insulin resistance in obesity and type 2 diabetes. Korean J Intern Med 2010;25:119-129. https://doi.org/10.3904/kjim.2010.25.2.119
  22. DeFronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes 1981; 30:1000-1007. https://doi.org/10.2337/diab.30.12.1000
  23. Peppa M, Koliaki C, Nikolopoulos P, Raptis SA. Skeletal muscle insulin resistance in endocrine disease. J Biomed Biotechnol 2010;2010:527850.
  24. Hardie DG. Role of AMP-activated protein kinase in the metabolic syndrome and in heart disease. FEBS Lett 2008;582:81-89. https://doi.org/10.1016/j.febslet.2007.11.018
  25. Misra P, Chakrabarti R. The role of AMP kinase in diabetes. Indian J Med Res 2007;125:389-398.
  26. Hwang JT, Kwon DY, Yoon SH. AMP-activated protein kinase: a potential target for the diseases prevention by natural occurring polyphenols. N Biotechnol 2009;26:17- 22. https://doi.org/10.1016/j.nbt.2009.03.005
  27. Winder WW, Hardie DG. AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. Am J Physiol 1999;277(1 Pt 1):E1-E10.
  28. Egawa T, Hamada T, Ma X, Karaike K, Kameda N, Masuda S, Iwanaka N, Hayashi T. Caffeine activates preferentially α1-isoform of 5'AMP-activated protein kinase in rat skeletal muscle. Acta Physiol (Oxf) 2011;201:227- 238. https://doi.org/10.1111/j.1748-1716.2010.02169.x
  29. Takikawa M, Inoue S, Horio F, Tsuda T. Dietary anthocyanin- rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. J Nutr 2010;140:527-533. https://doi.org/10.3945/jn.109.118216

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