Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Red Ginseng Saponin Fraction A Isolated from Korean Red Ginseng by Ultrafiltration on the Porcine Coronary Artery

  • Jung, Young-Hyun (Department of Physiology, Kyungpook National University College of Veterinary Medicine) ;
  • Park, Kwang-Yeol (Department of Physiology, Kyungpook National University College of Veterinary Medicine) ;
  • Jeon, Jin-Hong (Department of Physiology, Kyungpook National University College of Veterinary Medicine) ;
  • Kwak, Yi-Seong (Korea Ginseng Corporation Central Research Institute) ;
  • Song, Yong-Bum (Korea Ginseng Corporation Central Research Institute) ;
  • Wee, Jae-Joon (Korea Ginseng Corporation Central Research Institute) ;
  • Rhee, Man-Hee (Department of Physiology, Kyungpook National University College of Veterinary Medicine) ;
  • Kim, Tae-Wan (Department of Physiology, Kyungpook National University College of Veterinary Medicine)
  • Received : 2010.11.26
  • Accepted : 2010.12.09
  • Published : 2011.09.25
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Abstract

Red ginseng saponin fraction-A (RGSF-A) contains a high percentage of panaxadiol saponins that were isolated from Korean red ginseng by ultrafiltration. The aim of this study was to elucidate the effects of RGSF-A on the porcine distal left anterior descending (LAD) coronary artery. The relaxant responses to RGSF-A were examined during contractions induced by 100 nM U46619 (9,11-dideoxy-9a,11a-methanoepoxy-prostaglandin F2a), a stable analogue of thromboxane A2. RGSF-A dose-dependently induced biphasic (fast- and slow-) relaxation in the distal LAD coronary artery in the presence of an intact endothelium. The fast-relaxation was quickly achieved in a minute, and then the slow-relaxation was slowly developed and sustained for more than thirty minutes after the administration of RGSF-A. The slow-relaxation had a tendency to be bigger than the fast-relaxation. Fast relaxation induced by RGSF-A was almost blocked by $N_{\omega}$-Nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase synthase inhibitor and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a guanylate cyclase inhibitor. However slow relaxation induced by RGSF-A was only partially inhibited by L-NAME and ODQ. In the endothelium-removed ring, RGSF-A evoked only slowrelaxation to a certain extent. These data suggest that RGSF-A induced both endothelium dependent fast- and slow-relaxation and endothelium independent slow-relaxation in the porcine distal LAD coronary artery. The endothelium dependent fast-relaxation is mediated by the nitric oxide (NO)-cGMP pathway, and the endothelium dependent slow-relaxation is at least partially mediated by the NO-cGMP pathway. However, the endothelium-independent slow-relaxation remains to be elucidated.

Keywords

References

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