Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effect of Fructus Ligustri Lucidi $H_2O$ Extract on Cell Proliferation in Hman Dermal Fibroblast

여정실 물 분획물이 인체 진피 섬유아세포의 증식에 미치는 영향

  • Lim, Nan-Young (Dept. of Beauty Design Graduate School, Won-Kwang University) ;
  • Kim, Dae-Sung (Dept. of Anatomy, College of Oriental medicine, Won-Kwang University) ;
  • Ko, Kyung-Sook (Dept. of Beauty Design Graduate School, Won-Kwang University) ;
  • Mun, Yeun-Ja (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine, Won-Kwang University) ;
  • Woo, Won-Hong (Dept. of Herbal Resources, Professional Graduate School of Oriental Medicine, Won-Kwang University)
  • 임난영 (원광대학교 뷰티디자인학과) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 고경숙 (원광대학교 뷰티디자인학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실)
  • Received : 2011.08.27
  • Accepted : 2011.09.10
  • Published : 2011.09.27
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Abstract

Objectives : In this study, we investigated the effect of Fructus Ligustri Lucidi $H_2O$ fraction (FLLW) on cell proliferation, and the phosphorylation of ERKs and Akt in human dermal fibroblast neonatal (HDFn). Methods : After treatment of HDFn with FLLW, MTT assay was performed to quantitatively determine cellular viability. The ERK and Akt pathways were analyzed in vitro by Western blot in a HDFn. HDFn proliferation after FLLW and minoxidil treatment in the absence or presence of PD98059, a MEK inhibitor, LY294002, and a PI3K inhibitor, was examined by Western blot or MTT assay. Results : FLLW increased cell proliferation in a dose-dependent manner and minoxidil used as positive control also induced cell proliferation in HDFn. FLLW increased the phosphorylation of ERK and Akt. In addition, minoxidil, too, induced the phosphorylation of ERK and Akt in HDFn. PD98059 and LY294002 significantly attenuated FLLW-inducible p-ERK and p-Akt expression and proliferation in cultured HDFn. Conclusions : Our results suggest that FLLW stimulates the growth of fibroblast cells through ERK and Akt pathways. Therefore, FLLW is a potential agent for the inducer of fibroblast growth.

Keywords

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