Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Changes in Antioxidant Activity of Duck Egg after Pressurized Soaking in Green Tea Extract

녹차 추출물에 가압 침지한 오리알의 항산화 활성 변화

  • Choi, Young-Min (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Seon-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • 최용민 (충북대학교 농업환경생명대학 식품공학과) ;
  • 이선미 (충북대학교 농업환경생명대학 식품공학과) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 정헌상 (충북대학교 농업환경생명대학 식품공학과) ;
  • 이준수 (충북대학교 농업환경생명대학 식품공학과)
  • Received : 2011.06.20
  • Accepted : 2011.08.04
  • Published : 2011.09.30
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Abstract

This study was carried out to investigate changes in epigallocatechin gallate (EGCG) contents and antioxidant activity of duck egg after pressurized soaking in green tea extract. The duck eggs were soaked in different concentrations of green tea extract (10~30%) and subjected to pressures of 0.1~5.0 MPa for 30 min at ambient temperature in a lab model high-pressure rig. After pressured treatment at 5.0 MPa in 30% green tea extract, EGCG content of duck egg white (20 mg/100 g) markedly increased compared to that of untreated sample (0.17 mg/100 g). Moreover, the antioxidant, hepato-protective, and cellular antioxidant activities of duck egg white after pressured treatment at 5 MPa in 30% green tea extract were all higher than those of untreated sample. Our results could have a direct impact on duck egg consumption by increasing consumer awareness of the health benefits of duck eggs.

본 연구에서는 오리알에 녹차 추출물을 이용하여 가압침지처리를 함으로써 녹차의 기능성 성분을 침투시키고자 하였으며 가압침지 후 오리알의 성분변화와 항산화 활성의 변화를 연구하였다. 녹차의 활성성분을 함유하는 오리알을 제조하기 위해 녹차 10, 20, 30% 추출액에 오리알을 침지시켜 각각 1과 5 MPa 조건에서 30분 동안 상온에서 가압하여 녹차의 catechin 성분들이 침투되도록 하였다. 대조구(0.17 mg/100 g)와 비교하여 오리알 난백의 EGCG 함량은 20 mg/100 g(30% 녹차 추출물, 5 MPa)으로 약 100배 이상 증가하였으며 침지시료의 농도와 압력이 증가할수록 EGCG 함량이 증가함을 알 수 있었다. 항산화 활성과 세포 보호효과 및 ROS 제거 효과 모두 대조구보다 5 MPa 조건에서 증가하는 것으로 나타났다. 본 연구 결과는 비교적 간단한 공정과 경제적인 방법으로 녹차의 EGCG 함량이 증가된 오리알을 제조하는 방법을 연구하고 그 활성을 증명함으로써 국내 오리알을 이용한 고부가가치 가공식품 개발과 오리알의 소비촉진에 영향을 미칠 것으로 생각된다.

Keywords

References

  1. Lee MH, Park SJ. 1997. Distribution of the trace minerals in the wild duck egg. Korean J Sanit 12: 9-13.
  2. Ryu IH, Jung IT, Lee KS. 2004. Effect of drying condition of steamed egg yolk on acid value of duck egg yolk oil. Korean J Food Nutr 17: 206-211.
  3. Hong IJ, Yoon HK, Koo SJ. 1999. Analysis of lipid composition and fatty acids in poultry eggs-cage system, open barn system's hen egg, moscovy duck's egg, mallard's egg. Korean J Soc Food Sci 15: 645-651.
  4. Kim SH, Han D, Park JD. 2004. Changes of some chemical compounds of Korean green tea according to harvest periods. Korean J Food Sci Technol 36: 542-546.
  5. Hara Y, Watanabe M. 1989. Antibacterial activities of tea polyphenols against Clostridium botulinum. J Food Sci Technol 36: 951-955. https://doi.org/10.3136/nskkk1962.36.12_951
  6. Ryu BH, Park CO. 1997. Antioxidant effect of green tea extracts on enzymatic activities of hairless mice skin induced by ultraviolet B light. Korean J Food Sci Technol 29: 355-361.
  7. Landis-Piwowar KR, Huo C, Chen D, Milacic V, Shi1 G, Chan TH, Dou QP. 2007. A novel prodrug of the green tea polyphenol (-)-epigallocatechin-3-gallate as a potential anticancer agent. Cancer Res 67: 4303-4310. https://doi.org/10.1158/0008-5472.CAN-06-4699
  8. Sin MK, Han SH, Han GJ. 1997. The effects of green tea on the serum lipid and liver tissue of cholesterol fed rats. Korean J Food Sci Technol 29: 1255-1263.
  9. Kim DW, Kim JH, Kang GH, Kang HK, Choi JY, Kim SH, Kang CW. 2010. Effects of water extract mixtures from Artemisia capillaris, Camellia sinensis, Schizandra chinensis, and Viscum album var. coloratum on laying performance, egg quality, blood characteristics, and egg storage stability in laying hens. Korean J Food Sci Anim Resour 30: 449-457. https://doi.org/10.5851/kosfa.2010.30.3.449
  10. Sung HC, Suk YO, Han SM, Yu KH, Sung YK. 2002. Effect of feeding with chitosan on egg production rate and yolk cholesterol level. J Chitin Chitosan 7: 29-32.
  11. Lee CH, Nam KT, Kim JB, Han SH. 1996. The effects of extracts from Puerariae radix roots on the storage stability of egg and serum cholesterol level in the laying hens. Korean J Food Sci Anim Resour 16: 102-105.
  12. Park BS. 2004. Effect of dietary $\beta$-cyclodextrin on egg quality and cholesterol content of egg yolks. J Korean Soc Food Sci Nutr 33: 614-620. https://doi.org/10.3746/jkfn.2004.33.4.614
  13. Messens W, Camp JV, Huyghebaert AL. 1997. The use of high pressure to modify the functionality of food proteins. Trends Food Sci Technol 8: 107-112. https://doi.org/10.1016/S0924-2244(97)01015-7
  14. Okamoto M, Kawamura Y, Hayashr R. 1990. Application of high pressure to food processing: textural comparison of pressure and heat-induced gels of food proteins. Agric Biol Chem 54: 183-189. https://doi.org/10.1271/bbb1961.54.183
  15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  16. Mau JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35: 519-526. https://doi.org/10.1016/S0963-9969(01)00150-8
  17. Denizot F, Lang R. 1986. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89: 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  18. Wang H, Joseph JA. 1999. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 27: 612-616. https://doi.org/10.1016/S0891-5849(99)00107-0
  19. Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y. 1996. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radic Biol Med 21: 895-902. https://doi.org/10.1016/0891-5849(96)00237-7
  20. Davalos A, Miguel M, Bartolome B, Lopez-Fandino R. 2004. Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67: 1939-1944. https://doi.org/10.4315/0362-028X-67.9.1939
  21. Chen Y, Chang H, Wang C, Cheng F. 2009. Antioxidative activities of hydrolysates from duck egg white using enzymatic hydrolysis. Asian-Australasian J Anim Sci 22: 1587-1593. https://doi.org/10.5713/ajas.2009.90119
  22. Augustyniak A, Waszkiewicz E, Skrzydlewska E. 2005. Preventive action of green tea from changes in the liver antioxidant abilities of different aged rats intoxicated with ethanol. Nutrition 21: 925-932. https://doi.org/10.1016/j.nut.2005.01.006
  23. Kye IS, Jeon YS, No JK, Kim YJ, Lee KH, Shim KH, Kim J, Yokozawa T, Chung H. 1999. Reactive oxygen scavenging activity of green tea polyphenols. Korean J Gerontol 9: 10-17.

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