DOI QR코드

DOI QR Code

Scavenging Effect of Extract from Perilla frutescens and Rosmarinic Acid from Free Radical and Lipid Peroxidation

  • Wu, Ting Ting (Department of Food Science and Nutrition and Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Hwang, Bo-Ra (Department of Food Science and Nutrition and Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Cho, Eun-Ju (Department of Food Science and Nutrition and Research Institute of Ecology for the Elderly, Pusan National University)
  • Received : 2011.07.25
  • Accepted : 2011.09.05
  • Published : 2011.09.30

Abstract

The radical scavenging activity and inhibition effect from lipid peroxidation induced by peroxyl radical of methanol extract from Perilla frutescens and its active compound, rosmarinic acid (RA), were investigated in vitro. The treatment of extract and RA scavenged 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical (${\cdot}OH$) and nitric oxide in a concentration-dependent manner. In particular, the extract and RA showed strong radical scavenging activity against ${\cdot}OH$, the most toxic and reactive radical. In addition, Perilla frutescens and RA effectively inhibited lipid oxidation induced by sodium nitroprusside and 2,2'-azobis(2-aminopropane) dihydrochloride, determined by the ferric thiocyanate method. The present results suggest that Perilla frutescens and RA play a protective role against oxidative stress induced by free radical and lipid peroxidation.

Keywords

References

  1. Turner TT, Lysiak JJ. 2008. Oxidative stress: a common factor in testicular dysfunction. J Androl 29: 488-498. https://doi.org/10.2164/jandrol.108.005132
  2. Piao XS, Piao XL, Kim HY, Cho EJ. 2008. Antioxidative activity of geranium (Pelargonium inquinans Ait) and its active component, 1,2,3,4,6-penta-o-galloyl-beta-D-glucose. Phytother Res 22: 534-538. https://doi.org/10.1002/ptr.2398
  3. Halliwell B. 1987. Oxidants and human disease: some new concepts. FASEB J 1: 358-364. https://doi.org/10.1096/fasebj.1.5.2824268
  4. Slater TF. 1984. Free-radical mechanisms in tissue injury. Biochem J 222: 1-15. https://doi.org/10.1042/bj2220001
  5. Makino T, Furuta Y, Wakushima H, Fujii H, Saito K, Kano Y. 2003. Anti-allergic effect of Perilla frutescens and its active constituents. Phytother Res 17: 240-243. https://doi.org/10.1002/ptr.1115
  6. Banno N, Akihisa T, Tokuda H, Yasukawa K, Higashihara H, Ukiya M, Watanabe K, Kimura Y, Hasegawa J, Nishino H. 2004. Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects. Biosci Biotechnol Biochem 68: 85-90. https://doi.org/10.1271/bbb.68.85
  7. Heo JC, Nam DY, Seo MS, Lee SH. 2011. Alleviation of atopic dermatitis-related symptoms by Perilla frutescens Britton. Int J Mol Med 28: 733-737.
  8. Nakazawa T, Yasuda T, Ueda J, Ohsawa K. 2003. Antidepressant- like effects of apigenin and 2,4,5-trimethoxycinnamic acid from Perilla frutescens in the forced swimming test. Biol Pharm Bull 26: 474-480. https://doi.org/10.1248/bpb.26.474
  9. Ueda H, Yamazaki C, Yamazaki M. 2002. Luteolin as an anti-inflammatory and anti-allergic constituent of Perilla frutescens. Biol Pharm Bull 25: 1197-1202. https://doi.org/10.1248/bpb.25.1197
  10. Sanbongi C, Takano H, Osakabe N, Sasa N, Natsume M, Yanagisawa R, Inoue KI, Sadakane K, Ichinose T, Yoshikawa T. 2004. Rosmarinic acid in perilla extract inhibits allergic inflammation induced by mite allergen, in a mouse model. Clin Exp Allergy 34: 971-977. https://doi.org/10.1111/j.1365-2222.2004.01979.x
  11. Zekonis G, Zekonis J, Sadzeviciene R, Simoniene G, Kevelaitis E. 2008. Effect of Perilla frutescens aqueous extract on free radical production by human neutrophil leukocytes. Medicina 44: 699-705.
  12. Tada M, Matsumoto R, Yamaguchi H, Chiba K. 1996. Novel antioxidants isolated from Perilla frutescens Britton var. crispa (Thunb). Biosci Biotechnol Biochem 60: 1093- 1095. https://doi.org/10.1271/bbb.60.1093
  13. Dapkevicius A, Venskutonis R, Van Beek TA, Linssen JPH. 1998. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in lithuania. J Sci Food Agric 77: 140-146. https://doi.org/10.1002/(SICI)1097-0010(199805)77:1<140::AID-JSFA18>3.0.CO;2-K
  14. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T. 1989. Effects of the interaction of tannins with co-existing substances, VI: effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 37: 2016-2021. https://doi.org/10.1248/cpb.37.2016
  15. Chung SK, Osawa T, Kawakishi S. 1997. Hydroxyl radical- scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotech Biochem 61: 118-123. https://doi.org/10.1271/bbb.61.118
  16. Sreejayan, Rao MN. 1997. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 49: 105-107. https://doi.org/10.1111/j.2042-7158.1997.tb06761.x
  17. Kikuzaki H, Nakatani N. 1993. Antioxidant effect of some ginger constituents. J Food Sci 58: 1407-1410. https://doi.org/10.1111/j.1365-2621.1993.tb06194.x
  18. Sundararajan R, Haja NA, Venkatesan K, Mukherjee K, Saha BP, Bandyopadhyay A, Mukherjee PK. 2006. Cytisus scoparius link-a natural antioxidant. BMC Complement Altern Med 6: 8. https://doi.org/10.1186/1472-6882-6-8
  19. Formica JV, Regelson W. 1995. Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol 33: 1061-1080. https://doi.org/10.1016/0278-6915(95)00077-1
  20. Peng Y, Ye J, Kong J. 2005. Determination of phenolic compounds in Perilla frutescens L. by capillary electrophoresis with electrochemical detection. J Agric Food Chem 53: 8141-8147. https://doi.org/10.1021/jf051360e
  21. Osakabe N, Takano H, Sanbongi C, Yasuda A, Yanagisawa R, Inoue K, Yoshikawa T. 2004. Anti-inflammatory and anti-allergic effect of rosmarinic acid (RA); inhibition of seasonal allergic rhinoconjunctivitis (SAR) and its mechanism. Biofactors 21: 127-131. https://doi.org/10.1002/biof.552210125
  22. Ak T, Gülcin I. 2008. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 174: 27-37. https://doi.org/10.1016/j.cbi.2008.05.003
  23. Esmaeili MA, Sonboli A. 2010. Antioxidant, free radical scavenging activities of Salvia brachyantha and its protective effect against oxidative cardiac cell injury. Food Chem Toxicol 48: 846-853. https://doi.org/10.1016/j.fct.2009.12.020
  24. Aruoma OI, Grootveld M, Halliwell B. 1987. The role of iron in ascorbate-dependent deoxyribose degradation. Evidence consistent with a site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule. J Inorg Biochem 29: 289-299. https://doi.org/10.1016/0162-0134(87)80035-1
  25. Morbidelli L, Donnini S, Ziche M. 2004. Role of nitric oxide in tumor angiogenesis. In Angiogenesis in Brain Tumors. Kirsch M, Black PM, eds. Kluwer Academic Press, Boston, VA, USA. p 155-168.
  26. Li F, Wang FF, Yu F, Fang Y, Xin ZH, Yang FM, Xu J, Zhao LY, Hu QH. 2008. In vitro antioxidant and anticancer activities of ethanolic extract of selenium-enriched green tea. Food Chem 111: 165-170. https://doi.org/10.1016/j.foodchem.2008.03.057
  27. Ahmad R, Ali AM, Israf DA, Ismail NH, Shaari K, Lajis NH. 2005. Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sci 76: 1953-1964. https://doi.org/10.1016/j.lfs.2004.08.039
  28. Liu Q, Yao HY. 2007. Antioxidant activities of barley seeds extracts. Food Chem 102: 732-737. https://doi.org/10.1016/j.foodchem.2006.06.051
  29. Friederich JA, Butterworth JF. 1995. Sodium nitroprusside: twenty years and counting. Anesth Analg 81: 152- 162.
  30. Zou CG, Agar NS, Jones GL. 2001. Oxidative insult to human red blood cells induced by free radical initiator AAPH and its inhibition by a commercial antioxidant mixture. Life Sci 69: 75-86. https://doi.org/10.1016/S0024-3205(01)01112-2
  31. Miki M, Tamai H, Mino M, Yamamoto Y, Niki E. 1987. Free-radical chain oxidation of rat red blood cells by molecular oxygen and its inhibition by alpha-tocopherol. Arch Biochem Biophy 258: 373-380. https://doi.org/10.1016/0003-9861(87)90358-4
  32. Sato Y, Kamo S, Takahashi T, Suzuki Y. 1995. Mechanism of free radicals-induced hemolysis of human erythrocytes: hemolysis by water-soluble radical initiator. Biochemistry 34: 8940-8949. https://doi.org/10.1021/bi00028a002
  33. Chamulitrat W, Mason RP. 1989. Lipid peroxyl radical intermediates in the peroxidation of polyunsaturated fatty acids by lipoxygenase. Direct electron spin resonance investigations. J Biol Chem 264: 20968-20973.

Cited by

  1. Antioxidative and nitric oxide scavenging activity of branched-chain amino acids vol.24, pp.4, 2015, https://doi.org/10.1007/s10068-015-0200-2