DOI QR코드

DOI QR Code

Effects of spawning on immune functions in the surf clam Mactra veneriformis (Bivalvia: Mactridae)

  • Yu, Jin-Ha (National Fisheries Products Quality Inspection Service, Mokpo Branch) ;
  • Choi, Min-Chul (Incheon Fisheries Hatchery Research Institute) ;
  • Jung, Eun-Bin (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
  • 투고 : 2011.02.27
  • 심사 : 2011.04.22
  • 발행 : 2011.04.30

초록

The production of surf clam, Mactra veneriformis, an important fishery resource in Korea, has recently been decreasing. This study was carried out to examine effects of spawning on immune functions of this species. Total hemocyte count (THC), phenoloxidase (PO) activity, phagocytic activity, neutral red retention (NRR) time and antibacterial activity were assessed. Spawned clams showed reduction in THC, PO, phagocytic activity and NRR times compared with unspawned ones. While spawning event did not elicit any change of antibacterial activity in both spawned and unspawned ones. This study indicates that spawning process decreases immune functions in the surf clams which could cause mortality increment and yield reduction.

키워드

참고문헌

  1. Adema, C.M., van der Knaap, W.P.W. and Sminia, T.: Molluscan hemocyte-mediated cytotoxicity: the role of reactive oxygen intermideates. Res. Aquat. Sci., 4: 201-223, 1991.
  2. Aladaileh, S., Rodney, P., Nair, S.V. and Raftos, D.A.: Characterization of phenoloxidase activity in Sydney rock oysters (Saccostrea glomerata). Comp. Biochem. Physiol. B. 148: 470-480, 2007.
  3. Anderson, R.S.: Production of reactive oxygen intermediates by invertebrate hemocytes: immunological significance. In: Soderhall, K., Vasta, G., Iwanaga, S. (Eds.), New Directions in Invertebrate Immunology. SOS Publications, Fair Haven, NJ: 109-129, 1996.
  4. Anderson, R.S. and Beaven, A.E.: Antibacterial activities of oyster (Crassostrea virginica) and mussel (Mytilus edulis and Geukensia demissa) plasma. Aquat. Living Res., 14: 343-349, 2001. https://doi.org/10.1016/S0990-7440(01)01143-3
  5. Armugan, M., Romestand, B. and Torreilles, J.: Nitrite released in haemocytes from Mytilus galloprovincialis, Crassostrea gigas and Ruditapes decussates upon stimul;ation with phorbol myristate acetate. Aquat. Living Res., 13: 173-177, 2000. https://doi.org/10.1016/S0990-7440(00)00150-9
  6. Beatrice, G., Patrick, S., Patrick, M., Philippe, G., Olivier, L.M. and Tristan, R.: Diploid and triploid Pacific oyster Crassostrea gigas (Thunberg), reared at two heights above sediment in Marennes-Oleron Basin, France: Difference in mortality, sexual maturation, and hemocyte parameters. Aquaculture. 254: 606-616, 2006. https://doi.org/10.1016/j.aquaculture.2005.10.008
  7. Bertheline, C., Kellner, K. and Mathieu, M.: Storage metabolism in the Pacific Oyster (Crassostrea gigas) in relation to summer mortalities and reproductive cycle (west coast of France). Comp. Biochem. Physiol., 125B: 359-369, 2000.
  8. Borenfreud, E. and Puerner, J.A.: Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett., 24: 119-124, 1985. https://doi.org/10.1016/0378-4274(85)90046-3
  9. Carballal, M.J., Villaba, A. and Lopez, C.: Seasonal variation and effects of age, food availability, size, gonadal development and parasitism on the hemogram of Mytilus galloprovincialis. J. Invertebr. Pathol., 72: 304-312, 1998. https://doi.org/10.1006/jipa.1998.4779
  10. Cheng, T.C.: Selective induction of release of hydrolases from Crassostrea virginica hemocytes by certain bacteria. J. Invert. Pathol., 59: 197-200, 1992. https://doi.org/10.1016/0022-2011(92)90033-Z
  11. Cheng, T.C., Rodrick, G.E., Foley, D.A. and Koehler, S.A.: Release of lysozyme from hemolymph cells of Mercenaria mercenaria during phagocytosis. J. Invertr. Pathol., 25: 261-265, 1975. https://doi.org/10.1016/0022-2011(75)90076-2
  12. Chung E.Y., Kim J.H., Ryu D.K., Park G.M., Park K.H., Park S.W., So J.K., Shin Y.K., Lee J.S., Yi J.Y., Lee C.H., Choi J.W. and Huh Y.B.: Physiology and Ecology of bivalve mollusks. Seoul: Bioscience, 2008.
  13. Cho, S.M. and Jeong, W.G.: Spawning impact on lysosomal stability of the pacific oyster, Crassostrea gigas. Aquaculture. 244: 383-387, 2005. https://doi.org/10.1016/j.aquaculture.2004.12.013
  14. Delaporte, M., Soudant, P., Lambert, C., Moral, J., Pouvreau, S. and Samain, F.: Impact of food availability on energy storage and defense related hemocyte parameters of the Pacific oyster Crassostrea gigas during an experimental reproductive cycle. Aquaculture. 254: 571-582, 2006. https://doi.org/10.1016/j.aquaculture.2005.10.006
  15. Duchemin, M.B., Fournier, M. and Auffret, M.: Seasonal variation of immune parameters in diploid and triploid Pacific oysters, Crassostrea gigas (Thunberg). Aquaculture. 264: 73-81, 2007. https://doi.org/10.1016/j.aquaculture.2006.12.030
  16. Esteban, M.A., Munoz, P. and Moseguer, J.: Phenoloxidase activity in three commercial bivalve species. Changes due to natural infestation with Perkinsus atlanticus. Fish & Shellfish Immunol., 20: 12-19, 2006. https://doi.org/10.1016/j.fsi.2005.02.002
  17. Fishery Production Survey (FPS) (2009) : http://fs.fips.go.kr/ main.jsp.
  18. Hauton, C., Hawkins, L.E. and Hutchinson, S.: The use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L). Comp. Biochem. Physiol. B. 119: 619-623, 1998.
  19. LeBlanc, N., Nadry, T., Stryhn, H., Tremblay, R., McNiven, M. and Davidson, J.: The effect of high air and water temperature on juvenile Mytilus edulis in Prince Edward island, Canada. Aquaculture. 243: 185-194, 2005. https://doi.org/10.1016/j.aquaculture.2004.09.035
  20. Li, X., Zhang, Z., Vandepeer, M. and Zhao, W.: Effects of water temperature and air exposure on the lysosomal membrane stability of hemocytes in pacific oysters, Crassostrea gigas (Thunberg). Aquaculture. 256: 502-509, 2006. https://doi.org/10.1016/j.aquaculture.2006.02.003
  21. Li, Y., Qin, J.G., Abbott, C.A., Li, X. and Benkendorff, K.: Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: an explanation for summer mortality in Pacific oyster. Am J. Physio. Regul. Integ. Comp. Physiol., 293: 2253-2362, 2007.
  22. Li, Y., Qin, J.G., Li, X. and Benkendorff, K.: Spawning-dependant stress response to food deprivation in pacific oyster Crassostrea gigas. Aquaculture., 286: 309-317, 2009. https://doi.org/10.1016/j.aquaculture.2008.09.035
  23. Lowe, D.M. and Pipe, R.K.: Contaminant-induced lysosomal membrane damage in marine mussel digestive cells: an in vitro study. Aquat. Toxicol., 30: 357-365, 1994. https://doi.org/10.1016/0166-445X(94)00045-X
  24. Marin, M.G., Matozzo, V., Monari, M., Foschi, J., Papi, T. and Cattani, O.: Exposure to anoxia of the clam Chamelea gallina. I. Effects on immune responses. J. Exp. Mar. Biol. Eco., 325: 163-174, 2005. https://doi.org/10.1016/j.jembe.2005.04.030
  25. Marin, M.G., Monari, M., Matozzo, V., Foschi, J., Cattani, O., Serrazanetti, G.P.: Effects of high temperature on functional responses of haemocytes in the clam Chamelea gallina. Fish & Shellfish Immunol., 22: 1-17, 2007. https://doi.org/10.1016/j.fsi.2006.03.007
  26. Matozzo, V., Da Ros, L., Ballarin, L., Meneghetti, F. and Marin, M.G.: Functional response of haemocytes in the clam Tapes philippinarum from the Lagoon of Venice: fishing impact and seasonal variations. Can. J. Fisj. Aquat. Sci., 60: 949-958, 2003. https://doi.org/10.1139/f03-084
  27. Ordas, M.C., Ordas, A., Beloso, C. and Figueras, A.: Immune parameters in carpet shell clams naturally infected with Perkinsus atlanticus. Fish & Shellfish Immunol., 10: 597-609, 2000. https://doi.org/10.1006/fsim.2000.0274
  28. Park, S.W., Kang, S.G. and Kim, Y.G.: The morphology of the hemocytes of the clam, Ruditapes philippinarum (Mollusca: Bivalvia). J. Fish Pathol., 13: 129-136, 2000.
  29. Perdue, J.A., Beattie, J.H. and Chew, K.K.: Some relationship between gametogenic cycle and summer mortality phenomenon in the Pacific oyster (Crassostrea gigas) in Washington State. J. Shellfish Res., 1:9-16, 1981.
  30. Peters, R. and Raftos, D.A. (2003). The role of phenoloxidase suppression in QX disease outbreaks among Sydney rock oysters (Saccostrea glomerata). Aquaculture., 223, 29-39. https://doi.org/10.1016/S0044-8486(03)00169-8
  31. Pipe, R.K.: Hydrolytic enzymes associated with the granular haemoyctes of the marine mussel Mytilus edulis. Histochem. J., 22: 595-603, 1990. https://doi.org/10.1007/BF01072941
  32. Pipe, R.K. and Cole, J.A.: Environmental contaminants influencing immune function in marine bivalve mollusks. Fish & Shellfish Immunol., 5: 581-595, 1995. https://doi.org/10.1016/S1050-4648(95)80043-3
  33. Roch, P., Hubert, F., van der Knaap, W. and Noël, T.: Present knowledge on the molecular basis of cytotoxicity, antibacterial activity and stress response in marine bivalves. Ital. J. Zool., 63: 311-316, 1996. https://doi.org/10.1080/11250009609356151
  34. Suresh, K. and Mohandas, A.: Number and types of hemocytes in Sunetta scripta and Villorita cyprinoids var. cochinensis (Bivalvia), and leukocytosis subsequent to bacterial challenge. J. Invertebr. Pathol., 55: 312-318, 1990. https://doi.org/10.1016/0022-2011(90)90072-E
  35. Yu, J.H., Song, J.H., Choi, M.C. and Park, S.W.: Effects of water temperature change on immune function in surf clams, Mactra veneriformis (Bivalvia: Mactridae). J. Invert. Pathol., 102: 30-35, 2009. https://doi.org/10.1016/j.jip.2009.06.002
  36. Yu, J.H., Choi, M.C., Park, K.I. and Park, S.W.: Effects of anoxia on immune function in surf clam, Mactra veneriformis. Zool. Stud., 49: 94-101, 2010.

피인용 문헌

  1. Monitoring of VHS and RSIVD in Cultured Paralichthys olivaceus of Jeju in 2015 vol.49, pp.2, 2016, https://doi.org/10.5657/KFAS.2016.0176