Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Specific antibody response of olive flounder Paralichthys olivaceus by water temperature

수온에 따른 넙치의 특이 항체 반응

  • Kim, Wi-Sik (The Fisheries Science Institute, Chonnam National University) ;
  • Jang, Min-Seok (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Lee, Jeong-Ho (Genetics & Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Myeong, Jeong-In (Genetics & Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 김위식 (전남대학교 수산과학연구소) ;
  • 장민석 (전남대학교 수산생명의학과) ;
  • 정성주 (전남대학교 수산생명의학과) ;
  • 김석렬 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 병리연구과) ;
  • 이정호 (국립수산과학원 육종연구센터) ;
  • 명정인 (국립수산과학원 육종연구센터) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2010.12.09
  • Accepted : 2011.03.16
  • Published : 2011.04.30
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Abstract

The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of $15^{\circ}C$, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24~48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94~3.04. At the end of the experiment (84 days), 0.03~1.28 OD values were observed. In the rearing temperature of $12{\sim}13^{\circ}C$, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88~2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of $10^{\circ}C$, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37~1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.

본 연구에서는 넙치를 대상으로 항체 검출 ELISA법을 적용하기 위한 기초연구로서 넙치에 BSA를 면역시킨 후 사육 수온에 따른 특이 항체 반응을 항체 검출 ELISA법을 사용하여 조사하였다. 수온 $15^{\circ}C$에서는 면역 후 14일째부터 BSA에 대한 특이 항체가가 형성되는 개체가 확인되었고 (OD값: 0.69), 28~42일에 가장 높은 항체가 (OD값: 1.94~3.04)가 관찰되었으며, 면역 84일째는 0.03~1.28의 OD값이 관찰되었다. 수온 $12{\sim}13^{\circ}C$에서는 면역 후 28일째부터 항체가 관찰되었고 (OD값: 0.14~0.25), 3개체에서 56~70일에 가장 높은 항체가 (OD값: 1.88~2.68)가 확인되었으며, 면역 84일째에는 0.49~2.35의 OD값이 관찰되었다. 2개체의 경우는 면역 84일까지 0.8 이하의 OD값을 나타내었다. 수온 $10^{\circ}C$에서는 면역 후 56일째부터 항체가가 확인되었고 (OD값: 0.11~0.83), 2개체에서 면역 70일째 가장 높은 항체가가 (OD값: 1.37~1.53)가 확인되었으며, 면역 84일째에는 1.00~1.11의 OD값이 관찰되었다. 이에 반해 3개체에서는 항체가가 천천히 상승하여 면역 84일째 0.12~0.68의 OD값을 나타내었다. 이상의 결과로 넙치의 특이 항체 반응은 개체별로 차이를 보이나 수온이 높을수록 항체 형성이 빠르고 항체가도 높게 나타나며, 지속기간이 길게 나타나는 것이 확인되었다.

Keywords

References

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