YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effect of Eggplant Extract on Adipocyte Differentiation in 3T3-L1 Cells

가지 물추출물의 3T3-L1 지방전구세포 분화 억제효능

  • Lee, Mi-Kyeong (College of Pharmacy, Chungbuk National University) ;
  • Liu, Qing (College of Pharmacy, Chungbuk National University) ;
  • Hwang, Bang-Yeon (College of Pharmacy, Chungbuk National University) ;
  • Kim, Sun-Yeou (Graduate School of East-West Medical Science, Kyung Hee University Global Campus) ;
  • Lee, Jae-Hak (Korea Plant Resource Institute)
  • Received : 2011.04.25
  • Accepted : 2011.05.31
  • Published : 2011.08.31
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Abstract

Abnormal growth of adipocyte characterized by increased cell numbers and differentiation is considered as an major pathological characteristic feature in obesity. Thus, inhibition of mitogenesis of preadipocytes and their differentiation to adipocytes would be beneficial for the prevention and inhibition of obesity. In the present study, we attempted to evaluate anti-adipogenic activity of eggplants (the fruits of Solanum melongena L.) employing preadipocytes cell line, 3T3-L1 as an in vitro assay system. Water extract of eggplants significantly inhibited adipocyte differentiation when treated during adipocyte differentiation process, as assessed by measuring fat accumulation using Oil Red O staining. Eggplant extract, however, showed little effects on fully differentiated adipocytes. Eggplant didn't show significant toxicity up to 500 ${\mu}g$/ml to the 3T3-L1 cells. Further studies with interval treatment demonstrated that eggplant exerted inhibitory activity on adipocyte differentiation via acting on early stage of adipogenesis. Conclusively, eggplants are suggested to be beneficial for the prevention of obesity.

Keywords

References

  1. Grundy, S. M. : Multifactorial causation of obesity: implications for prevention. Am. J. Clin. Nutr. 67, 563s (1998). https://doi.org/10.1093/ajcn/67.3.563S
  2. Bastard, J. P., Maachi, M., Lagathu, C., Kim, M. J., Caron, M., Vidal, H., Capeau, J. and Feve, B. : Recent advances in the relationship between obesity, inflammation and insulin resistance. Eur. Cytokine Netw. 17, 4 (2006).
  3. Kopelman, P. G. : Obesity as a medical problem. Nature 404, 635 (2000). https://doi.org/10.1038/35007508
  4. Rayalam, S., Della-Fera, M. A. and Baile, C. A. : Phytochemicals and regulation of the adipocyte life cycle. J. Nutr. Biochem. 19, 717 (2008). https://doi.org/10.1016/j.jnutbio.2007.12.007
  5. Spielgelman, B. M. and Enerback, S. : The adipocyte: a multifunctional cell. Cell Metab. 4, 425 (2006). https://doi.org/10.1016/j.cmet.2006.11.009
  6. Rosen, E. D. and Spiegelman, B. M. : Adipocytes as regulators of energy balance and glucose homeostasis. Nature 444, 847 (2006). https://doi.org/10.1038/nature05483
  7. Akanitapichat, P., Phraibung, K., Nuchklang, K. and Prompitakkul, S. : Antioxidant and hepatoprotective activities of five eggplant varieties. Food Chem. Toxicol. 48, 3017 (2010). https://doi.org/10.1016/j.fct.2010.07.045
  8. 배기환 : 한국의 약용식물, 교학사, 서울, p 456.
  9. Ben-Erik van Wyk : Food plants of the world, Briza publications, Pretoria, p 347.
  10. Mennella, G., Rotino, G. L., Fibiani, M., D'Alessandro, A., Francese, G., Toppino, L., Cavallanti, F., Acciarri, N. and Lo Scalzo, R. : Characterization of health-related compounds in eggplant (Solanum melongena L.) lines derived from introgression of allied species. J. Agric. Food Chem. 58, 7597 (2010). https://doi.org/10.1021/jf101004z
  11. Azuma, K., Ohyama, A., Ippoushi, K., Ichiyanagi, T., Takeuchi, A., Saito, T. and Fukouka, H. : Structure and antioxidant activity of anthocyanins in many accessions of eggplant and its related species. J. Agric. Food Chem. 56, 10154 (2008). https://doi.org/10.1021/jf801322m
  12. Kwon, Y. I., Apostolides, E. and Shetty, K. : In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresour. Technol. 99, 2981 (2008) https://doi.org/10.1016/j.biortech.2007.06.035
  13. Shin, E., Choi, K. H., Yoo, H. S., Lee, C. K., Hwang, B. Y. and Lee, M. K. : Inhibitory effects of coumarins from the stem barks of Franxinus rhynchophylla on adipocyte differentiation in 3T3-L1 cells. Biol. Pharm. Bull. 33, 1610 (2010). https://doi.org/10.1248/bpb.33.1610
  14. Lee, M. K., Shin, E., Liu, Q., Hwang, B. Y., Lee, J., Kim, S. Y. and Lee, J. H. : Inhibitory activity of three varieties of adlay (coix seed) on adipocyte differentiation in 3T3-L1 cells. Nat. Prod. Sci. 16, 291 (2010).
  15. Gregoire, F. M., Smas, C. M. and Sul, H. S. : Understanding adipocyte differentiation. Physiol. Rev. 78, 783 (1998). https://doi.org/10.1152/physrev.1998.78.3.783
  16. Lefterova, M. I. and Lazar, M. A. : New developments in adipogenesis. Trends Endocrinol. Metab. 20, 107 (2009). https://doi.org/10.1016/j.tem.2008.11.005
  17. Farmer, S. R. : Transcriptional control of adipocyte formation. Cell Metab. 4, 263 (2006). https://doi.org/10.1016/j.cmet.2006.07.001
  18. White, U. A. and Stephens, J. M. : Transcriptional factors that promote formation of white adipose tissue. Mol. Cell. Endocrinol. 318, 10 (2010). https://doi.org/10.1016/j.mce.2009.08.023
  19. Kim, S. O., Yun, S. J., Jung, B., Lee, E. H., Hahm, D. H., Shim, I. and Lee, H. J. : Hypolipidemic effects of crude extract of adlay seed (Coix lachrymal-jabi L.var. ma-yuen Stapf) in obesity rat fed high fat diet: Relationship of TNF-$\alpha$and leptin mRNA expressions and serum lipid levels. Life Sci. 75, 1391 (2004). https://doi.org/10.1016/j.lfs.2004.03.006
  20. Wolfram, S., Wang, Y. and Thielecke, F. : Anti-obesity effects of green tea: from bedside to bench. Mol. Nutr. Food Res. 50, 176 (2006). https://doi.org/10.1002/mnfr.200500102