Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative Properties of Ethanolic Extracts from Flowering Cherry (Prunus serrulata L. var. spontanea Max. wils.) Fruit with Various Doses of Gamma Radiation

감마선 조사선량에 따른 버찌(Fruit of Prunus serrulata L. var. spontanea Max. wils.) 에탄올 추출물의 항산화활성

  • Kim, Kyung-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Jo, Ji-Eun (Dept. of Food and Nutrition, Chungnam National University) ;
  • Lee, Seong-A (Dept. of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
  • 김경희 (충남대학교 식품영양학과) ;
  • 조지은 (충남대학교 식품영양학과) ;
  • 이성아 (충남대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2011.07.26
  • Accepted : 2011.09.21
  • Published : 2011.10.31
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Abstract

This study investigated the antioxidant activity of ethanolic extracts of 1~20 kGy-gamma irradiated flowering cherry fruit (Prunus serrulata L. var. spontanea Max. wils.). The total phenolic compound content was highest (30.35 mg/g) in the 10 kGy sample, but there was no consistent trend matching the increase or decrease of gamma irradiation. Radical scavenging activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) showed that gamma-irradiated samples were better than non-irradiated samples and that the 10 kGy-gamma irradiated sample was the best ($IC_{50}$ 327.52 ppm). In reducing power, gamma-irradiated samples induced higher activities than non-irradiated samples at concentrations below 2.5 mg/mL, and the sample with the highest activity was the 20 kGy-gamma irradiated sample. In nitrite-scavenging ability, both gamma-irradiated and non-irradiated samples showed high activity (73~94%) at pH 1.2, 3.0, and 4.2. Also, the nitrite-scavenging ability of gamma-irradiated samples was significantly different from that of the control at pH 1.2. The FRAP value of the 3 kGy-irradiated sample also showed the highest value of 0.38 mM. Our results indicate that gamma irradiating the ethanolic extracts of flowering cherry fruit increases the antioxidant activity at some of the radiation doses. Therefore, it suggests that gamma irradiation could increase biological activity and be used effectively in food manufacturing and related industries.

본 연구는 폐자원으로 버려지는 버찌의 활용 증대방안 모색을 위한 기초자료 마련을 위해 버찌의 에탄올 추출물에 0~20 kGy 선량으로 감마선 조사를 실시하고, 이에 따른 항산화활성 변화를 측정하였다. 총 폴리페놀 함량 측정결과, 10 kGy 조사구에서 30.35 mg/g으로 가장 높은 값을 나타내었으나 감마선 조사에 따른 일정한 증가나 감소의 경향은 나타내지 않았다. DPPH radical 소거능 측정결과 10 kGy 조사구에서 327.52 ppm으로 가장 낮은 $IC_{50}$값을 나타내 가장 높은 활성을 나타내었으며 대체적으로 비조사구보다는 조사구에서 높은 활성을 나타내었다. 환원력 측정 결과, 2.5 mg/mL 이하 농도에서 비조사구에 비해 조사구에서 높은 활성을 나타내었고 20 kGy 조사구에서 대체적으로 높은 환원력을 나타내었다. 아질산염 소거능을 측정한 결과 pH 4.2 이하의 pH에서 조사구 및 비조사구 모두 73~94% 이내의 높은 소거능을 나타내 pH 의존적인 아질산염 소거능을 보였으나 조사에 따른 활성 증가는 나타내지 않았다. FRAP value를 측정결과 3 kGy 조사구에서 0.38 mM로 가장 높은 활성을 나타내었다. 결론적으로 버찌 에탄올 추출물에 대한 감마선 조사는 일부 선량에서 추출물의 항산화 활성 증가를 나타내며, 사용목적에 따른 적정 선량의 감마선 조사는 생리활성물질의 증가를 유도하여 산업적인 면에서 효율적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

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