Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Aceriphyllum rossii Ethanol Extract on Lipid Metabolism in Rats Fed a High-Fat Diet

돌단풍 에탄올 추출물이 고지방식이를 급여한 흰쥐의 지질대사에 미치는 영향

  • Park, Yu-Hwa (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Hee-Yeon (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Lim, Sang-Hyun (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Kyung-Hee (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Park, Dong-Sik (National Academy of Agricultural Science) ;
  • Lee, Jung-Hoon (Dept. of Herbal Crop Research, NIHHIS, RDA) ;
  • Park, Chung-Geon (Dept. of Herbal Crop Research, NIHHIS, RDA) ;
  • Park, Chung-Berm (Dept. of Herbal Crop Research, NIHHIS, RDA) ;
  • Kim, Song-Mun (Dept. of Biological Environment, Kangwon National University)
  • Received : 2011.08.19
  • Accepted : 2011.09.09
  • Published : 2011.10.31
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Abstract

In this study, we investigated the anti-obesity activity of Aceriphyllum rossii ethanol extract on rat fed a high fat diet. Male SD rats were divided into four groups. Group 1 was the control. Group 2 was fed a high-fat diet. Group 3 was the positive control, fed a high-fat diet supplemented with Garcinia Cambogia extracts. Group 4 was fed a high fat diet supplemented with ethanol extracts of Aceriphyllum rossii (EEAR). Precisely 166 mg/kg of powdered Garcinia Cambogia extracts was used for Group 3. Also, 250 mg/kg of EEAR was used for Group 4. The Body weight increased Group 2, but decreased Group 4. The serum total cholesterol in Group 2 increased by 15.26% when compared to Group 1, but only increased 5.29% in Group 3 and 4.29% in Group 4. The liver and mesenteric adipose tissue weights of Group 2 increased compared to Group 1, whereas they decreased in Group 3 and Group 4. As a result of measuring the concentration of triglycerides in extracted livers, Group 2 showed a significant increase compared to the Group 1, and Groups 3 and 4 showed significant decrease compared Group 2. These results suggest that Aceriphyllum rossii ethanol extracts may be useful as an anti-obesity agent.

본 연구의 목적은 돌단풍 에탄올 추출물의 지질대사 개선 및 항비만 효과에 대해 구명하고자 4주령 된 흰쥐 40마리를 고지방 사료 급여로 비만을 유도한 후 정상군, 고지방 식이대조군, 돌단풍 에탄올 추출물 처리군, 양성 대조군(가르시니아)으로 나누어 8주간 실시하였다. 고지방 식이로 인하여 체중이 유의적으로 증가하였고, 돌단풍 에탄올 추출물은 고지방식이만을 투여한 대조군과 비교 시 9.68% 유의적으로 감소하였으며, 양성대조군인 가르시니아 투여군과 유의적으로 같은 수준이었다. 돌단풍 에탄올 추출물이 식이섭취량에는 영향을 미치지 않았으며, 식이효율은 고지방식이만을 투여한 대조군이 정상군에 비하여 유의적으로 증가하였고, 돌단풍 에탄올 추출물 투여군과 양성대조군은 유의적으로 같은 수준이었으며 고지방식이만을 투여한 대조군에 비하여 유의적으로 감소하였다. 혈청 중 총콜레스테롤 함량은 고지방 식이만을 투여한 대조군이 정상군 대비 15.26% 유의적으로 증가하였고, 돌단풍 에탄올 추출물 투여군과 양성대조군인 가르시니아 투여군은 고지방 식이만을 투여한 대조군 대비 각각 11.46, 10.53% 유의적으로 감소하였다. 혈청 중 중성지방 함량은 고지방 식이만을 투여한 대조군이 정상군 대비 34.79% 유의적으로 증가하였다. 돌단풍 에탄올 추출물 투여군과 양성 대조군인 가르시니아 투여군은 고지방식이만을 투여한 대조군 대비 각각 9.02, 6.45% 감소하였으나 유의차는 없었다. 간과 복강 내 지방 조직의 무게는 돌단풍 에탄올 추출물 급여로 유의하게 감소하였으나, 부고환지방 조직의 무게는 유의차가 없었다. 간 조직 내 중성지방의 경우 고지방 식이만을 투여한 대조군에서 정상군 대비 21.39% 유의적으로 증가함을 알 수 있었다. 또한 돌단풍 에탄올 추출물 처리군과 양성대조군에서는 고지방식이만을 투여한 대조군 대비 각각 12.83, 10.52% 유의적으로 감소하였다. 위의 실험 결과 돌단풍 에탄올 추출물은 체중, 간, 복강내 지방조직의 감소와 지질대사 개선에 효과적으로 작용하였으며, 향후 돌단풍은 비만 예방 및 치료에 효과적인 소재로 활용될 수 있을 것으로 판단된다.

Keywords

References

  1. Flegal KM, Carroll MD, Kuczmarski RJ, Johnson CL. 1998. Overweight and obesity in the United States: prevalence and trends, 1960-1994. Int J Obes Relat Metab Disord 22: 39-47. https://doi.org/10.1038/sj.ijo.0800541
  2. Ahn IS, Park KY, Do MS. 2007. Weight control mechanisms and antiobesity functional agents. J Korean Soc Food Sci Nutr 36: 503-513. https://doi.org/10.3746/jkfn.2007.36.4.503
  3. Jeon SM, Choi MS. 2010. Comparison of the effects of cyclodextrin- naringin inclusion complex with naringin on lipid metabolism in mice fed a high-fat diet. J East Asian Soc Dietary Life 20: 20-29.
  4. Bray GA. 2004. Medical consequences of obesity. J Clin Endocrinol Metab 89: 2583-2589. https://doi.org/10.1210/jc.2004-0535
  5. Park JY, Kim JY, Lee SP, Lee JH. 2010. The effect of green coffee bean extract supplementation on body fat reduction in overweight/obese women. Korean J Nutr 43: 374-381. https://doi.org/10.4163/kjn.2010.43.4.374
  6. Rolls BJ, Shide DJ, Thorwart ML, Ulbert JS. 1988. Sibutramine reduces food intake in nondieting women with obesity. Obes Res 6: 1-11.
  7. Hansen DL, Toubro S, Stock MJ, Macdonald IA, Astrup A. 1988. Thermogenic effects of sibutramine in humans. Am J Clin Nutr 68: 1180-1186.
  8. Ransac S, Gargouri Y, Moreau H, Verger R. 1991. Inactivation of pancreatic and gastric lipases by tetrahydrolistatin and alkyl-dithio-5-(2-nitrobenzoic acid). A kinetic study with 1,2-didecanoyl-sn-glycerol monolayers. Eur J Biochem 202: 395-400. https://doi.org/10.1111/j.1432-1033.1991.tb16387.x
  9. Apfelbaum M, Vague P, Ziegler O, Hanotin C, Thomas F, Leutenegger E. 1999. Long-term maintenance of weight loss after a very low calorie diet: efficacy and tolerability of sibutramine. Am J Med 106: 179-184. https://doi.org/10.1016/S0002-9343(98)00411-2
  10. Yum KS. 2001. Orlistat (XenicalR). Korean J Obesity 10: 25-36.
  11. Ahn EM, Han JT, Kwon BM, Kim SH, Baek NI. 2008. Anticancer activity of flavonoids from Aceriphyllum rossii. J Korean Soc Appl Biol Chem 51: 309-315. https://doi.org/10.3839/jksabc.2008.054
  12. Lee WC. 1996. Aceriphyllum rossii. In Standard Illustration of Korean Plants. 1st ed. Academy Press, Seoul, Korea. p 147-156.
  13. Han JT, Kim HY, Park YD, Lee YH, Lee KR, Kwon BM, Baek NI. 2002. Aceriphyllic acid A, a new ACAT inhibitory triterpenoid from Aceriphyllum rossii. Planta Med 68: 558-561. https://doi.org/10.1055/s-2002-32565
  14. Han JT, Bang MH, Chun OK, Kim DO, Lee CY, Baek NI. 2004. Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities. Arch Pharm Res 27: 390-395. https://doi.org/10.1007/BF02980079
  15. Min BS, Lee IS, Chang MJ, Yoo JK, Na MK, Hung TM, Thoung PT, Lee JP, Kim JH, Kim JC, Woo MH, Choi JS, Lee HK, Bae KH. 2008. Anticomplementary activity of triterpenoids from the whole plant of Aceriphyllum rossii against the classical pathway. Planta Med 74: 726-729. https://doi.org/10.1055/s-2008-1074534
  16. Lee IS, Yoo JK, Na MK, Min BS, Lee JP, Yun BS, Jin WY, Bae KH. 2007. Cytotoxity of triterpenes isolate from Aceriphyllum rossii. Chem Pharm Bull 55: 1376-1378. https://doi.org/10.1248/cpb.55.1376
  17. Lee CH, Lee JH, Heo CS. 2007. $\beta$-site amyloid precursor protein cleaving enzyme inhibitory activity of Aceriphyllum rossii. Korea Patent 10-2007-0022745.
  18. Lee HS, Lee CH, Kim YK, No MC, Park HL, Seo JH, Choi L, Yoo MY. 2008. Metabolic pharmaceutical composition comprising Aceriphyllum rossii extract and active compound isolated from them. Korea Patent 10-2008-0002433.
  19. Reeves PG, Nielson FH, Fahey Jr GC. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951.
  20. Folch J, Less M. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226: 497-509.
  21. Lee JJ, Park MR, Kim AR, Lee MY. 2011. Effects of ramie leaves on improvement of lipid metabolism and anti-obesity effect in rats fed a high fat/high cholesterol diet. Korean J Food Sci Technol 43: 83-90. https://doi.org/10.9721/KJFST.2011.43.1.083
  22. Lee JS, Lee MK, Ha YT, Bok SH, Park HM, Jeong KS, Woo MN, Do GM, Yeo JY, Choi MS. 2006. Supplementation of whole persimmon leaf improves lipid profiles and suppressed body weight gain in rats fed high-fat diet. Food Chem Toxicol 44: 1875-1883. https://doi.org/10.1016/j.fct.2006.06.014
  23. Hang JY, Choi HJ. 2003. Effects of Artermisia lwayomogi oilgosaccharide on the blood lipids, abdominal adipose tissues, and leptin levels in the obese rats. Korean J Nutr 36: 437-445.
  24. Park PJ, Kim CW, Cho SY, Rha CS, Seo DB, Lee SJ. 2010. Aqueous spray-dried green tea extract regulates body weight and epididymal fat accumulation in mice. Korean J Food Sci Technol 42: 103-108.
  25. Smith EB. 1974. The relationship between plasma and tissue lipid in human atherosclerosis. Adv Lipid Res 11: 1-7.
  26. De Leo ME, Landriscina M, Palazzotti B, Borrello S, Galeotti T. 1997. Iron modulation of LPS-induced manganese superoxide dismutase gene expression in rat tissues. FEBS Lett 403: 131-135. https://doi.org/10.1016/S0014-5793(97)00034-3
  27. Sollof LA, Futenberg HL, Lacko AG. 1973. Serum cholesterol esterification in patients with coronary artery disease. Am Heart J 85: 153-161. https://doi.org/10.1016/0002-8703(73)90455-9
  28. Park PJ, Kim CW, Cho SY, Rha CS, Seo DB, Lee SJ. 2010. Aqueous spray-dried green tea extract regulates body weight and epididylmal fat accumulation in mice. Korean J Food Sci Technol 42: 103-108.
  29. Kim AR, Lee JJ, Lee YM, Jung HO, Lee MY. 2010. Cholesterol-lowering and anti-obesity effects of Polymnia sonchifolia Poepp. & Endl. powder in rats fed a high fat-high cholesterol diet. J Korean Soc Food Sci Nutr 39: 210-218. https://doi.org/10.3746/jkfn.2010.39.2.210
  30. Yoon HJ, Park YS. 2010. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J Korean Soc Food Sci Nutr 39: 219-226. https://doi.org/10.3746/jkfn.2010.39.2.219

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