Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant and Anti-inflammatory Activities of Extracts from Korean Traditional Medicinal Prescriptions

한방처방제추출물의 항산화 및 항염증 활성

  • Lee, Sung-Gyu (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Lee, Eun-Ju (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Park, Woo-Dong (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Kim, Jong-Boo (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 이성규 ((주)엔유씨전자 바이오연구소) ;
  • 이은주 ((주)엔유씨전자 바이오연구소) ;
  • 박우동 ((주)엔유씨전자 바이오연구소) ;
  • 김종부 ((주)엔유씨전자 바이오연구소) ;
  • 최상원 (대구가톨릭대학교 식품영양학과)
  • Received : 2011.05.03
  • Accepted : 2011.07.20
  • Published : 2011.10.31
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Abstract

Water and ethanol extracts of 16 different Korean traditional oriental prescriptions used widely for prevention of degenerative arthritis were prepared and their total phenolic and total flavonoid contents were quantified. Additionally, antioxidant and anti-inflammatory activities of water, ethanol and enzyme-treated extracts were determined by in vitro assays. Total phenolic and total flavonoid contents of three extracts from the 16 different medicinal prescriptions varied from 10.03-78.03 and 0-16.24 mg/g, respectively. Among the three extracts of 16 different medicinal prescriptions tested, 'Mahangeuigamtang' showed the potent full term for DPPH ($RC_{50}$=71.26, 27.33, 63.00 ${\mu}g/mL$) and full term for ABTS ($RC_{50}$=21.11, 27.45, 152.11 ${\mu}g/mL$) radical scavenging activities, and its water and ethanol extracts exhibited significant cyclooxygenase-2 (COX-2) inhibitory activity (49.10 and 69.06%, respectively). Ethanol and enzyme-treated extracts of 'Euieuiintang' exerted the strongest COX-2 inhibitory activity (68.23 and 75.05%, respectively). 'Mahangeuigamtang' and 'Euieuiintang' may be useful as potential therapeutic agents for treatment of degenerative diseases, such as inflammation and aging.

본 연구에서는 민간 및 한방에서 관절염 치료 처방제로 널리 사용되고 있는 한방처방제 16종을 선정하여 향후 관절염 예방 및 치료용 기능성식품의 개발 기초 자료로 활용하기 위해 한방처방제로부터 물, 에탄올 및 효소처리추출물을 제조하고 그들의 항산화 및 항염증활성을 in vitro assay system을 사용하여 측정하였다. 16종 한방처방제의 각 추출물의 폴리페놀 함량은 10.03-78.03 mg/g의 범위를 나타내었으며, 이 중 마행의감탕(7번)의 에탄올추출물이 78.03 mg/g으로 16종의 한방처방제 중 가장 높은 폴리페놀 함량을 보였다. 총 플라보노이드 함량의 경우 총 폴리페놀 함량의 결과와 달리 효소추출물에서는 폴리페놀 추출 함량이 낮아지거나 비슷한 수치를 보였지만 총 플라보노이드 함량의 경우 마행의감탕(7번)을 제외한 나머지 15종의 한방처방제에서 열수 및 에탄올추출물보다 효소추출물에서 플라보노이드 함량이 증가하는 것을 확인하였다. 한편, 마행의감탕(7번) 추출물에서 DPPH 및 ABTS 라디컬 포착활성과 COX-2 저해활성도 가장 높게 나타난 반면, 효소추출물에서는 의의인탕(14번)이 가장 높은 COX-2 저해활성을 나타내었다. 이러한 결과를 종합해 볼 때 마행의감탕 및 의의인탕을 관절염 치료 한방처방제로 개발할 가능성이 높다고 생각되며, 향후 보다 상세한 그들의 항산화 및 항염증활성을 조사할 필요가 있다고 생각된다.

Keywords

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