Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa

참나물의 항균, 항산화 활성 및 대장암세포 성장억제 활성 평가

  • Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University) ;
  • Kim, Mi-Sun (Dept. of Food and Nutrition, Andong National University) ;
  • Jung, In-Chang (Dept. of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 안선미 (안동대학교 식품영양학과) ;
  • 김미선 (안동대학교 식품영양학과) ;
  • 정인창 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2011.01.28
  • Accepted : 2011.07.15
  • Published : 2011.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Pimpinella brachycarpa, called as cham-na-mul in Korea, is an edible popular herb. However, the study of biological activity of P. brachycarpa is still rudimentary in worldwide. In this study, from the cultivated P. brachycarpa, we prepared the methanol extract and its subsequent solvent fractions, and their antimicrobial, antioxidation, and anti-proliferative activities were evaluated. The fraction yields of n-hexane (H), methylene chloride (EC), ethylacetate (EA), butanol (B), and water residue (W) from the methanol extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of total flavonoid and total polyphenol showed that the EA fraction contained the highest contents (89.23 and 200 mg/g), and the W residue has the lowest contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity assay, the EA fraction showed a broad-range antibacterial activity, while the H fraction is effective against gram positive bacteria. In antioxidation activity assay, EA and B fraction showed strong DPPH anion and ABTS cation scavenging activities including reducing power, and Hand MC fraction showed effective nitrite scavenging activity (71.43~83.82 ${\mu}g$/mL of $IC_{50}$). In a while, only B fraction showed strong anti-proliferative activity against human colorectal cancer HCT-116 (166 ${\mu}g$/mL of $IC_{50}$) as a dose-dependent manner up to 200 ${\mu}g$/mL. These results suggest that the EA and B fraction of P. brachycarpa could be developed as functional food ingredients.

참나물은 광범위하게 이용되는 식용산채임에도 불구하고 그 연구는 전 세계적으로 미미한 상태이다. 본 연구에서는 최근 고급 식용 산채로서 각광받고 있는 참나물의 메탄올 추출물을 조제하고, 이로부터 n-hexane, methylene chloride, ethylacetate 및 butanol을 이용하여 순차적 유기 용매 분획물과 물 잔류물을 조제하여 각각의 항산화, 항균 및 대장암세포 생육억제 활성을 평가하였다. 참나물 메탄올 추출물의 71.51%는 물 잔류물로 이행되어 추출물 대부분이 수용성 물질이었으며, n-hexane, methylene chloride, ethylacetate 및 butanol 분획 효율은 각각 18.71, 0.7, 0.56, 및 4.57%로 나타났다. 총 플라보노이드 및 총 폴리페놀 함량은, 분획수율이 가장 낮은 ethylacetate 분획에서 가장 높았으며 물 잔류물에서 가장 낮았다. 항균 활성 평가결과, ethylacetate 분획에서 그람양성 및 그람음성세균에 대해 광범위한 항균 활성을 나타내었으며, 특히 그람양성세균에는 n-hexane 분획이, 그람음성세균에는 ethylacetate 분획이 효과적이었다. 항산화 활성의 경우 ethylacetate 및 butanol 분획에서 양호한 DPPH 및 ABTS 소거능, 환원력을 나타내었으며, n-hexane및 methylene chloride 분획에서 우수한 nitrite 소거능을 나타내었다. 한편 butanol 분획에서는 다른 분획과는 달리 우수한 인간 대장암세포 생육억제 활성을 나타내었다. 본 연구 결과는 참나물을 이용한 기능성 식품개발 및 ethylacetate 분획을 대상으로 한 유용 소재개발의 기본 자료로 활용될 것이다.

Keywords

References

  1. Chang KM (2007) A study cookery utilization of Pimpinella brachycarpa N for developing as functional foods. Kor J Food Culture, 22, 274-282
  2. Choi MH, Kim GH (2002) A study on quality characteristics of Pimpinella brachycarpa kimchi during storage at different temperatures. J Kor Soc Food Sci Nutr, 31, 45-49 https://doi.org/10.3746/jkfn.2002.31.1.045
  3. Choi NS, Oh SS, Lee JM (2001) Change of biologically functional compounds of Pimpinella brachycarpa (Chamnamul) by blanching conditions. Kor J Dietary Culture, 16, 388-397
  4. Chang KM, Chung MS, Kim MK, Kim GH (2007) Analysis of mineral and volatile compounds in Pimpinella brachycarpa N by ICP-AES and SDE,HS-SPME-GC-MS. Kor J Food Culture, 22, 246-253
  5. Lee JJ, Choo MH, Lee MY (2007) Physicochemical compositions of Pimpinella brachycarpa. J Kor Soc Food Sci Nutr, 36, 327-331 https://doi.org/10.3746/jkfn.2007.36.3.327
  6. Kwon SJ, Hong SW, Kim NS, Kim JC (2004) Isolation of callus-specific mRNAs from differentiating embryogenic somatic calli of Pimpinella brachycarpa by cDNA-AFLP. Mol Cells, 17, 39-44
  7. Son SI, Kim JC (1999) Cloning and characterization of homeodomain-zip gene, Phc5 in embryogenic callus derived from Pimpinella brachycarpa suspension cultured cells. Kor J Plant Tissue Culture, 26, 121-126
  8. Cha YR, Lee KW, Moon YH, Kim JC, Han TJ, Lee WS, Cho SH (1998) Cloning of a cDNA encoding phospholipase D from Pimpinella brachycarpa. Mol Cells, 8, 19-26
  9. Lee MK, Kim SH, Ham SS, Lee SY, Chung CK, Kang IJ, Oh DH (2000) The effect of far infrared ray-vacuum drying on the quality changes of Pimpinella brachycarpa. J Kor Soc Food Sci Nutr, 29, 561-567
  10. Song HS, Choi HS, Lee MS (1997) Usefulness of Pimpinella brachycarpa as natural spice by sensory analysis. Kor J Food Sci, 13, 669-673
  11. Lee YM, Lee JJ, Lee MY (2008) Antioxidative effect of Pimpinella brachycarpa ethanol extract. J Life Sci, 18, 467-473 https://doi.org/10.5352/JLS.2008.18.4.467
  12. Choo MH, Lee JJ, Lee MY (2007) Effect of Pimpinella brachycarpa ethanol extract on chronically ethanol- induced liver damage in rats. J Life Sci, 17, 1406-1413 https://doi.org/10.5352/JLS.2007.17.10.1406
  13. Lee JJ, Choo MH, Lee MY (2006) Effect of Pimpinella brachycarpa extract on lipid metabolism in rats fed high cholesterol diet. J Kor Soc Food Sci Nutr, 35, 1151-1158 https://doi.org/10.3746/jkfn.2006.35.9.1151
  14. Kwon CS, Kwon YS, Kim YS, Kwon GS, Jin I, Ryu GC, Sohn HY (2004) Inhibitory activities of edible and medicinal herbs against human thrombin. J Life Sci, 14, 509-513 https://doi.org/10.5352/JLS.2004.14.3.509
  15. Kim JI, Jang HS, Kim JS, Sohn HY (2009) Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Kor J Microbiol Biotechnol, 37, 133-139
  16. Ahn SM, Ryu HY, Kang DK, Jung IC, Sohn HY (2009) Antimicrobial and antioxidant activity of the fruit of Prunus avium L. Kor J Microbiol Biotechnol, 37, 371-376
  17. Sohn HY, Kum EJ, Ryu HY, Jeon SJ, Kim NS, Son KH (2006) Antifungal activity of fistulosides, steroidal saponins, from Allium fistulosum L. J Life Sci, 16, 310-314 https://doi.org/10.5352/JLS.2006.16.2.310
  18. Kum EJ, Park SJ, Lee BH, Kim JS, Son KH, Sohn HY (2006) Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscorea batatas Decne. J Life Sci, 16, 647-652 https://doi.org/10.5352/JLS.2006.16.4.647
  19. Sohn HY, Kum EJ, Kwon YS, Kwon GS, Jin I, Kwon HY, Kwon CS, Son KH (2003) Screening of anti-candidiosis agent from medicinal and wild plants. J Life Sci, 13, 604-617 https://doi.org/10.5352/JLS.2003.13.5.604
  20. Ryu HY, Ahn SM, Shin YK, Sohn HY (2010) Antimicrobial and hemolytic activity of oriental medicinal herbs. Kor J Microbiol Biotechnol, 38, 190-1976
  21. Ahn SM, Hong YK, Kwon GS, Sohn HY (2011) Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts. J Life Sci, 21, 576-583 https://doi.org/10.5352/JLS.2011.21.4.576
  22. Sohn HY, Shin YK, Kim JS (2010) Anti-proliferative activities of solid-state fermented medcinal herbs using Phellinus baumii against human colorectal HCT116 cell. J Life Sci, 20, 1268-1275 https://doi.org/10.5352/JLS.2010.20.8.1268
  23. Sohn HY, Kwon CS, Kwon GS, Lee JB, Kim E (2004) Induction of oxidative stress by endosulfan and protective effect of lipid-soluble antioxidants against endosulfan- induced oxidative damage. Toxicol Lett, 151, 357-365 https://doi.org/10.1016/j.toxlet.2004.03.004
  24. Ryu HY, Ahn SM, Kim JS, Jung IC, Sohn HY (2010) Antimicrobial activity of fruit of Crataegus pinnatifida Bunge against multidrug resistant pathogenic Pseudomonas aeruginosa and Candida sp. Kor J Microbiol Biotechnol, 38, 77-83
  25. Sohn HY, Son KH, Kwon CS, Kwon GS, Kang SS (2004) Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine, 11, 666-672 https://doi.org/10.1016/j.phymed.2003.09.005

Cited by

  1. Phytochemicals That Regulate Neurodegenerative Disease by Targeting Neurotrophins: A Comprehensive Review vol.2015, 2015, https://doi.org/10.1155/2015/814068
  2. Physicochemical Changes in Hemerocallis coreana Nakai After Blanching, Drying, and Fermentation vol.42, pp.10, 2013, https://doi.org/10.3746/jkfn.2013.42.10.1638
  3. Antioxidant and Physicochemical Changes in Salvia plebeia R. Br. after Hot-air Drying and Blanching vol.43, pp.6, 2014, https://doi.org/10.3746/jkfn.2014.43.6.893
  4. Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation vol.29, pp.1, 2013, https://doi.org/10.1016/j.foodcont.2012.05.060
  5. Quinic acid derivatives from Pimpinella brachycarpa exert anti-neuroinflammatory activity in lipopolysaccharide-induced microglia vol.23, pp.7, 2013, https://doi.org/10.1016/j.bmcl.2013.01.115
  6. Two New Sesquiterpenes from the Aerial Parts of Pimpinella brachycarpa NAKAI vol.34, pp.7, 2013, https://doi.org/10.5012/bkcs.2013.34.7.2215
  7. Changes in Physicochemical Properties of Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. by Blanching, Drying, and Fermentation vol.44, pp.3, 2015, https://doi.org/10.3746/jkfn.2015.44.3.425
  8. 곰취 열수 추출물의 항산화 및 항염증 활성 vol.24, pp.6, 2017, https://doi.org/10.11002/kjfp.2017.24.6.834
  9. 추출방법을 달리한 곰취(Ligularia fischeri) 추출물의 항산화 및 생리활성에 관한 연구 vol.24, pp.8, 2017, https://doi.org/10.11002/kjfp.2017.24.8.1113
  10. Improvement of Phenylpropanoid Production with Elicitor Treatments in Pimpinella brachycarpa Nakai vol.6, pp.4, 2020, https://doi.org/10.3390/horticulturae6040108