Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Whitening and Anti-wrinkle Effects of Apple Extracts

사과 추출물의 미백 및 주름개선 효과

  • Jeong, Hee-Rok (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jo, Yu-Na (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji-Hee (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong-Eun (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Song, Byung-Gi (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 정희록 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원) ;
  • 조유나 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원) ;
  • 정지희 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원) ;
  • 진동은 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원) ;
  • 송병기 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원) ;
  • 허호진 (경상대학교 농업생명과학대학 식품공학과, 농업생명과학연구원)
  • Received : 2011.01.19
  • Accepted : 2011.07.15
  • Published : 2011.08.30
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Abstract

The in-vitro whitening and anti-wrinkle effect of ethanol extracts from apple flesh and peel were investigated. The EtOAc fractions from the ethanol extracts of apple flesh and peel showed in-vitro antioxidant activities in a dose-dependent manner on ABTS radical-scavenging activity and ferric-reducing/antioxidant power, and had the highest total phenolic contents (84.25 and 318.25 mg GAE/g). In addition, the EtOAc fractions generally showed strong UV absorption within the UV-B range. In the cellular system, the melanin synthesis of the B16/F10 melanoma cells was decreased by the EtOAc fractions of apple peel in a concentration-dependent manner. The EtOAc fractions of apple peel also showed a great elastase inhibition of 46.40% at 100 ${\mu}g$/mL, thus showing good in-vitro anti-wrinkle characteristics. These results suggest that the EtOAc fractions from the ethanol extract of apple peel can be used as whitening and anti-wrinkle agents as well as antioxidant resources.

본 연구에서는 상대적으로 우수한 총 페놀화합물 함량(84.25, 318.25 mg GAE/g)을 나타낸 사과 과육 및 과피의 EtOAc 분획물의 in vitro 항산화 효과, 미백 및 주름개선 효과를 알아보기 위해 다양한 연구를 진행하였다. 사과 과육 및 과피의 EtOAc 분획물의 ABTS radical 소거활성과 FRAP assay결과 농도 의존적인 경향이 나타났으며, 과피에서 높은 in vitro 항산화 활성을 보여주었다. 미백 효능을 알아보기 위한 자외선 흡수도 측정 결과 역시 두 시료 모두 UV-B (270-290 nm) 영역을 효과적으로 흡수하였으며, 특히 과피의 경우 UV-A (350-370 nm) 영역에서도 높은 흡수도를 보여주었다. 또한 멜라닌 함량 저해 효과를 분석한 결과 두 시료에서 모두 농도 의존적인 멜라닌 함량의 감소를 나타냈으나 특히 과피의 경우 positive control로서의 arbutin보다도 높은 저해 효과를 보여주었다. 더불어 사과 과피의 EtOAc 분획물은 500 ${\mu}g$/mL의 농도에서 46.40%의 elastase 저해활성 역시 보여주었다. 본 연구결과를 종합해 볼 때 다량의 페놀성 화합물을 함유한 사과 과피의 EtOAc 분획물은 항산화, 미백 및 주름개선 기능성화장품 소재로서의 활용 가치가 높다고 판단된다.

Keywords

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