Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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A Microbiological Analysis and Hazard Factor Evaluation of Food Utensils and Fixtures of Food Service Operations in Schools

학교 집단급식소 내 식기류 및 집기류의 미생물학적 분석 및 위해요인 평가

  • Park, Sung-Jun (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Hong, Sung-Ho (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Lee, Anne Ha-Young (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kim, Cheol-Ju (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kim, Su-Jin (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kim, Sung-Kyoon (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Ko, Gwang-Pyo (Department of Environmental Health, Graduate School of Public Health, Seoul National University)
  • 박성준 (서울대학교 보건대학원 환경보건학과) ;
  • 홍성호 (서울대학교 보건대학원 환경보건학과) ;
  • 이하영 (서울대학교 보건대학원 환경보건학과) ;
  • 김철주 (서울대학교 보건대학원 환경보건학과) ;
  • 김수진 (서울대학교 보건대학원 환경보건학과) ;
  • 김성균 (서울대학교 보건대학원 환경보건학과) ;
  • 고광표 (서울대학교 보건대학원 환경보건학과)
  • Received : 2011.10.04
  • Accepted : 2011.10.21
  • Published : 2011.10.31
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Abstract

Objectives: The aim of this study was to evaluate the microbial hazards posed by food utensils and fixtures in food service operations at selected middle and high schools located in Seoul, Korea. Methods: We collected 200 samples of utensils and fixtures including cups, spoons/chopsticks, food trays and tables from five different schools in Seoul. Target microorganisms of this study were divided into two groups: total bacterial count and total coliform as indicators of microbial contamination and Bacillus cereus and Staphylococcus aureus as pathogens of food poisoning. We used selective media to quantify microbial concentration and 16S rRNA PCR assay for qualitative analysis. In addition, intensive interviews with nutritionists were conducted and observations were made to identify factors that may affect microbial contamination. Logistic regression analysis was employed to examine the relationship between the microbial concentration and operation characteristics of each operation. Results: The level of microbial concentration in school B and C were significantly lower than in school A, D and E (p<0.05). Some samples from school A, D and E showed over 3.4 log CFU/100 $cm^2$ (total bacterial count) and 1.0 log CFU/100 $cm^2$ (total coliform), which requires immediate hygienic action. The number of customers per staff member, periodicity of hygiene education for staff and daily operation time of sterilizers were also found to be important factors related with the microbial contamination of food service operations. Conclusions: These results suggested that not only a HACCP (Hazard Analysis and Critical Control Point) approach, but also efforts to assess internal risk factors within operations be needed to reduce the microbial contamination of food utensils and fixtures. This study is expected to provide preliminary data for assessing microbial hazards in food service operations.

Keywords

References

  1. Kim JG. A Survey on the Sanitary Condition of Kitchens in School Food-service Programs. Kor J Env Health. 2003; 29(2): 87-93.
  2. Ministry of Education, Science and Technology, Korea. Current Status of School Foodservice programs in 2010. Available: http://www.mest.go.kr/ web/1111/ko/board/ view.do?bbsId=150&mode=view &board- Seq=23282 [accessed 20 July 2011].
  3. Korea Human Rights Foundation. The Present Status of student rights since 2008. Available: http://www.humanrights.or.kr/bbs/bbs/board.php?bo_table =bbs3&wr_id=37&sfl=&stx=&sst=wr_datetime&sod=asc&sop=and&page=3 [accessed 23 July 2011].
  4. Kim KY, Nam MJ, Nam BR, Ryu HJ, Heo RW, Shim WB et al. Microbiological Safety Assessment to Secure Safety of Food Service in University. J Fd Hyg Safety. 2010; 25(1): 49-58.
  5. Song IS, Chae IS. The Analysis of the School Foodservice Employees' knowledge and Performance Degree of HACCP System in Jeju. Korean J Nutr. 208; 41(8): 870-886.
  6. Korea Food & Drug Administration. The System of Food Poisoning Statistics, 2010. Available: http://e-stat.kfda.go.kr/ [accessed 20 June 2011].
  7. Hwang SS, Chon MY, Kim JM, Choi SH, Lee JA, Yoo IY et al. Hygiene.Safety and HACCP system by Management Type of School Food Service. J Korean Soc Living Environ Sys. 2008; 15(3): 372-382.
  8. Mokhtari A, Jaykus LA. Quantitative Exposure Model for the Transmission of Norovirus in Retail Food Preparation. Int J Food Microbiol. 2009; 133(1-2): 38-47. https://doi.org/10.1016/j.ijfoodmicro.2009.04.021
  9. Korea Food & Drug Administration. Current Status of Sanitation of Food Courts, 2011. Available: http://www.kfda.go.kr/index.kfda?mid=56&seq=14940& cmd=v [accessed 19 June 2011].
  10. Jeong DK, Lyu ES. The Microbiological Evaluation of Environments and Facilities at Food Service Operations in Elementary School. J Korea Soc Food Sci Nutr. 2002; 31(2): 216-220. https://doi.org/10.3746/jkfn.2002.31.2.216
  11. Torrence ME, Isaacson RE. Microbial Food Safety in Animal Agriculture: Current Topics. Wiley- Blackwell; 2003. p.275-280
  12. Park JY, Kim JS, Nam BR, Kim JG. A Study on the Hand Washing Awareness and Practices of Food-service Employees and the Load of Index Microorganisms on the Hands. J Env Health Sci. 2010; 36(2): 95-107.
  13. Ministry of Education, Science and Technology, Korea. Guidelines of Sanitation Management for School Foodservice Operations. 2010; 3: 1-130
  14. Food and Drug Administration, U.S. Bacteriological Analytical Manual. Available: http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/default.htm [accessed 30 June 2011].
  15. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ et al. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Appl Environ Microbiol. 1998; 64(2): 795-799.
  16. Sites JE, Walker PN, Burke A, Annous BA. Development and Validation of a Pilot Scale Enhanced Biosafety Level Two Containment for Performance Evaluation of Produce Disinfection Technologies. Applied Biosafety. 2008; 13(1): 30-44. https://doi.org/10.1177/153567600801300106
  17. Harrigan WF, McCance ME. The examination of food processing plant. In Laboratory methods in food and dairy microbiology. Academic Press, London, England; 1976. p.231-236
  18. Ehrlich R, Miller S, Walker RL. Relationship Between Atmospheric Temperature and Survival of Airborne Bacteria. Appl Microbiol. 1970; 19(2): 245-249.
  19. Jawad A, Heritage J, Snelling AM, Gascoyne-Binzi DM, Hawkey PM. Influence of relative humidity and suspending menstrua on survival of Acinetobacter spp. on dry surfaces. J Clin Microbiol. 1996; 34(12): 2881-2887.
  20. Park SH, Lim YH. A Study on the Sanitary Management of School Foodservice Operations in Daejeon and Chungnam. Korean J Community Nutr. 2005; 10(2): 234-242.
  21. Kim JG, Park JY, Kim JS. A Comparison of Microbial Load on Bare and Gloved Hands among Food Handlers. J Environ Health Sci. 2011; 37(4): 298- 305. https://doi.org/10.5668/JEHS.2011.37.4.298
  22. Park HJ, Bae HJ. Evaluation of Microbiological Hazards of Hygiene by the Customer Hands in University Foodservice Operation. J Korean Soc Food Sci Nutr. 2006; 35(7): 940-944. https://doi.org/10.3746/jkfn.2006.35.7.940
  23. Kjolen H, Andersen BM. Handwashing and disinfection of heavily contaminated hands-effective or ineffective? J Hospital Infect. 1991; 21(1): 61-70.
  24. Park JY, Kim JS, Kim JG. A Study in the Hand Washing Awareness and Practive of Food-service Employees and the Load of Index Microorganisms on the Hands. J Environ Health Sci. 2010; 36(2): 95-107.

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