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A Study on Change of Excess Volume in Membrane of Holobacterium Holobium

Halobacterium Halobium의 Membrane에서 잉여부피 변화에 관한 연구

  • Kim, Ki-Jun (Dept. of Chemical Engineering, Dae-Jin University) ;
  • Lee, Joo-Youb (Dept. of Chemical Engineering, Dae-Jin University)
  • 김기준 (대진대학교 공과대학 화학공학과) ;
  • 이주엽 (대진대학교 공과대학 화학공학과)
  • Received : 2011.06.09
  • Accepted : 2011.06.23
  • Published : 2011.06.30

Abstract

The excess volumes of mixing of benzyl alcohol and halothane in vesicle and in suspensions of several lipids have been determined at $25^{\circ}C$ it using a excess volume dilatometer. The potency of general anesthetics has long been known to correlate with lipid solubility. Denaturations of the vesicle, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by absorption changes at 280 nm and fluorescence changes at 330 nm and excess volume dilatometer. The particle size analysis of viscous polymer solutions by diffusional interchange is the key step by measurement. The excess volume of mixing in chitosan was found to be negative, whereas them of purple membrane, Halobacteriun Halobium and red membrane were positive in benzyl alcohol and halothane. This result was confirmed as Miller's supposition.

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References

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