Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect of Kenpaullone, a Specific Inhibitor of GSK3${\beta}$, on Melanin Synthesis in B16 Melanoma and Human Melanocytes

GSK3${\beta}$의 선택적 저해제인 Kenpaullone의 B16 멜라노마 및 인간 멜라노사이트에서의 영향

  • Kim, Hae-Jong (Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University) ;
  • Lee, You-Ree (Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University) ;
  • Nguyen, Dung Hoang (Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University) ;
  • Lee, Hyang-Bok (Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University) ;
  • Kim, Eun-Ki (Department of Biological Engineering, National Research Lab of Skin Bioactive Material, Inha University)
  • 김해종 (인하대학교 바이오피부신소재실험실) ;
  • 이유리 (인하대학교 바이오피부신소재실험실) ;
  • 호앙구엔 (인하대학교 바이오피부신소재실험실) ;
  • 이향복 (인하대학교 바이오피부신소재실험실) ;
  • 김은기 (인하대학교 바이오피부신소재실험실)
  • Received : 2011.06.20
  • Accepted : 2011.09.19
  • Published : 2011.09.30
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Abstract

Effects of Kenpaullone, a specific inhibitor of GSK3${\beta}$, on melanin synthesis in B16 melanoma cells and human melanocytes were investigated. Kenpaullone showed a melanogenesis stimulation activity in a concentrationdependent manner in murine B16 melanoma cells and human melanocytes without any significant effects on cell proliferation. Tyrosinase activity was increased 48 h after treatment of B16 cells with Kenpaullone. The protein expression level of tyrosinase was dose-dependently enhanced after the treatment with Kenpaullone. At the same time, the expression level of tyrosinase mRNA was also increased after addition of Kenpaullone. The stimulatory effect of Kenpaullone mainly resulted from increased expression of tyrosinase. These findings suggest that the application of GSK3${\beta}$ inhibitors may be a potential therapeutic agent for the treatment of hypopigmentation disorder.

Glycogen synthase kinase 3 beta (GSK3${\beta}$)의 선택적 저해제인 Kenpaullone가 B16 멜라노마 및 사람의 멜라노사이트에 미치는 멜라닌 합성능을 조사하였다. Kepaullone은 B16 멜라노마 및 사람의 멜라노사이트에 대하여 세포증식에는 영향이 없는 범위 내에서 농도 의존적으로 멜라닌 합성을 촉진시켰다. B16 멜라노마 세포에 Kenpaullone을 첨가 48 h 후 tyrosinase 활성이 증가하였으며, 농도별 처리에 대하여 tyrosinase 단백질의 발현 및 tyrosinase mRNA양이 증가함을 관찰하였다. 결론적으로 Kenpaullone는 B16 멜라노마 세포에서 tyrosinase 효소의 발현을 증가시켜 멜라닌 합성을 촉진하는 것으로 판단되어진다. 따라서 GSK3${\beta}$ 저해제가 멜라닌 합성을 촉진시키는 결과는 백반증과 같은 저색소관련 질병의 치료제 개발의 가능성을 갖고 있는 소재로서 응용가능하리라고 판단되어진다.

Keywords

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