Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Characterization of a New High-lysine Mutant in Barley (Hordeum vulgare L.)

  • Kim, Hong-Sik (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dea-Wook (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Sun-Lim (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Baek, Seong-Bum (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Hyoung-Ho (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Hwang, Jong-Jin (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Si-Ju (Upland Crop Division, National Institute of Crop Science, Rural Development Administration)
  • Received : 2011.10.19
  • Accepted : 2011.12.12
  • Published : 2011.12.30
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Abstract

A chemical, MNU-induced hulless barley mutant line designated as 'Mutant 98 (M98)' was developed from a Korean hulless waxy barley cultivar, 'Chalssalbori'. The objective of the study was to determine the genetic basis of 'M98' and the possibility of using 'M98' as breeding parent to improve lysine level. Compared to 'Chalssalbori', 'M98' had large embryo and higher lysine content in both the embryo and endosperm. Significantly different lysine content in 'M98' and the other high-lysine barley mutant stocks was observed for two years. However, the genotype by year interaction was not significant. 'M98' was higher than the other high-lysine barley mutant stocks in the percentage of lysine of total amino acid composition (0.75%). The trait of shrunken endosperm of 'M98', which was typical in the high-lysine mutants, was inherited by a single recessive gene. Based on seed morphology and lysine content of $F_1$ seeds, 'M98' had a genetically different gene from the other high-lysine mutants for shrunken endosperm. Segregation of $F_2$ for plump/shrunken endosperm did not fit the expected ratio of Mendelian inheritance except for only one cross combination (GSHO1784 (lys1)/M98). The amino acid analysis of $F_5$ and $F_6$ progenies from the cross between 'M98' and 'Chalssalbori' revealed that the attempt to increase the range of lysine content of plump lines did not go beyond the limit of the average high-lysine barley germplasm.

Keywords

Acknowledgement

Supported by : International Atomic Energy Agency

References

  1. Ahokas H. 1979. Further results on the suppression of shrunken endosperm in high lysine mutants. Barley Genet. Newsl. 9:3-7.
  2. Ahokas H. 1988. High-lysine gene segregation distorted in the barley cross Riso 1508 x Crypt CI 1090: Patterns of endosperm proteins by an electrophoretic method. Hereditas 108:129-131.
  3. Doll H. 1991. A factor on barley chromosome 7 causing distorted male segregation of gene lys3a. Barley Genet. Newsl. 20:23-24.
  4. Doll H. 1976. Genetic studies of high-lysine mutants. In: Barley Genetics III. Verlag Karl Thiemig, Munchen, Germany, pp.542-546.
  5. Doll H, Oram R. 1989. Deviating Mendelian segregation of barley gene lys3a. Hereditas 110:97-99.
  6. Eagles HA, Bedggood AGJ, Panozzo F, Martin PJ. 1995. Cultivar and environmental effects on malting quality in barley. Aust. J. of Agri. Res. 46:831-844. https://doi.org/10.1071/AR9950831
  7. Eggum BO, Brunsgaard G, Jensen J. 1995. The nutritive value of new high-lysine barley mutants. J of Cereal Sci. 22:171-176. https://doi.org/10.1016/0733-5210(95)90047-0
  8. Ewart JAD. 1967. Amino acid analyses of cereal flour proteins. J. of the Sci. of Food and Agri. 18:548-552. https://doi.org/10.1002/jsfa.2740181112
  9. FAO. 1973. Energy and protein requirements, FAO Nutrition Meetings Report, Series No. 52, WHO Technical Report Series No. 552, Rome.
  10. Gabert VM, Jorgensen H, Brunsgaard G, Eggum BO, Jensen J. 1996. The nutritional value of new high-lysine barley varieties determined with rats and pigs. Can. J. of Anim. Sci. 76:443-450. https://doi.org/10.4141/cjas96-064
  11. Jacobsen S, Sondergaard I, Moller B, Desler T, Munck L. 2005. A chemometric evaluation of the underlying physical and chemical patterns that support near infrared spectroscopy of barley seeds as a tool for explorative classification of endosperm genes and gene combinations. J. of Cereal Sci. 42:281-299. https://doi.org/10.1016/j.jcs.2005.02.008
  12. Jensen J, Doll H. 1979. Gene symbols for barley highlysine mutants. Barley Genet. Newsl. 9:33-37.
  13. Kim JG, Suh SJ, Baek SB, Kang MS. 2000. Genetic and chemical analysis of barley mutant with large embryo and shrunken endosperm. 8th Int'l Barley Genet. Symp. III, pp.160-161.
  14. Kim SL, Son BY, Jung TW, Moon HG, Son JR. 2006. Characterization on fatty acids and amino acids of quality protein maize lines. Korean J. Crop Sci. 51:458-465.
  15. Kreis M, Shewry PR, Forde BG, Rahman S, Miflin BJ. 1983. Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (Hordeum vulgare). Cell. 34:161-167. https://doi.org/10.1016/0092-8674(83)90146-0
  16. Krishnan HB. 1999. Characterization of high-lysine mutants of rice. Crop Sci. 39:825-831. https://doi.org/10.2135/cropsci1999.0011183X003900030035x
  17. Masaya F, Masamitsu I, Eiji D, Naoyuki I, Yoshinori D. 1999. Breeding of high-lysine naked barley, Parental line No. 2. Bull. of Shikoku Nat'l Agri. Exp. Stn. 64:9-20.
  18. Muench SR, Lejeune AJ, Nilan RA, Kleinhofs A. 1975. Evidence for two independent high lysine genes in barley. Barley Genet. Newsl. 5:31-33.
  19. Munck L. 1992. The case of high lysine barley breeding. Biotechnol-Agric. Wallingford, Oxford, UK: CAB International. pp.573-601.
  20. Munck L, Karlsson KE, Hagberg A, Eggum BO. 1970. Gene for improved nutritional value in barley seed protein. Science 168:985-987. https://doi.org/10.1126/science.168.3934.985
  21. Oram RN, Doll H. 1981. Yield improvement in high lysine barley. Aust. J. of Agri. Res. 32:425-434. https://doi.org/10.1071/AR9810425
  22. Oria MP, Hamaker BR, Axtell JD. 2000. A highly digestible sorghum cultivar exhibits a unique folded structure of endosperm protein bodies. Proc. Nat'l. Acad. Sci. USA. 97:5065-5070. https://doi.org/10.1073/pnas.080076297
  23. Park EJ, Kang JH, Kim KH. 2008. Characterization of amino acid contents in grains of core collection of Korean native rice. Korean J. Breed. Sci. 40:269-277.
  24. Shewry PR. 2007. Improving the protein content and composition of cereal grain. J. of Cereal Sci. 46:239-250. https://doi.org/10.1016/j.jcs.2007.06.006
  25. Shewry PR, Faulks AJ, Miflin BJ. 1980. Effect of high-lysine mutations on the protein fractions of barley grain. Biochem. Genet. 18:133-151. https://doi.org/10.1007/BF00504365
  26. Tallberg A, Eggum BO. 1986. Grain yields and nutritional qualities of some high-lysine barley hybrids. J. of Cereal Sci. 4:345-352. https://doi.org/10.1016/S0733-5210(86)80038-8
  27. Ullrich SE, Eslick RF. 1978. Allelism evidence for barley high lysine, shrunken endosperm xenia (sex) mutants. Barley Genet. Newsl. 8:109-112.
  28. Vasal SK. 2002. Quality protein maize: Overcoming the hurdles. J. of Crop Prod. 6:193-227. https://doi.org/10.1300/J144v06n01_11
  29. Xue DW, Dong J, Wu FB, Zhang GP. 2008. Genotypic and environmental variation in grain protein components and their relations to beta-amylase and beta-glucanase activity in malting barley. Cereal Res. Comm. 36:125- 134. https://doi.org/10.1556/CRC.36.2008.1.13
  30. Yamae G, Konishi T. 1993. Distorted segregation of the lys3a mutant in barley kernels. Barley Genet. Newsl. 22:68-69.