Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Activities of Solvent Fractions from Methanolic Extract of Cockscome (Celosia cristata L.) Flowers

맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

  • Kim, Hyun Young (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Ko, Jee Yeon (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Song, Seuk Bo (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Jung In (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Seo, Hye In (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jae Saeng (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Kwak, Do Yeon (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Jung, Tae Wook (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ki Young (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Oh, In Seok (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Jeong, Heon Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan Sik (Dept. of Functional Crop, National Institute of Crop Science, Rural Development Administration)
  • 김현영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 고지연 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 송석보 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 김정인 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 서혜인 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이재생 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 곽도연 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정태욱 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 김기영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 오인석 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 우관식 (농촌진흥청 국립식량과학원 기능성작물부)
  • Received : 2012.07.30
  • Accepted : 2012.10.04
  • Published : 2012.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 mg gallic acid equivalent (GAE)/g ME, 2.34 mg catechin equivalent (CE)/g ME, 6.23 mg tannic acid equivalent (TAE)/g ME, and $44.72{\mu}g$ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 mg GAE/g solvent fraction (SF), 5.44 mg CE/g SF, and 13.38 mg TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and $41.80{\mu}g$ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 mg Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 mg TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 mg GAE/g ME, 2.34 mg CE/g ME 및 6.23 mg TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 mg GAE/g SF, 5.44 mg CE/g SF 및 13.38 mg TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 $44.72{\mu}g$ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 $41.80{\mu}g$ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 mg TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 mg TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다.

Keywords

References

  1. Pyo YH, Yoon MY, Son JH, Choe TB. 2008. The effect of Celosia cristata L. ethanol extract on anti-oxidant & anti-aging activity. Korean J Biotechnol Bioeng 23: 431-438.
  2. Palada MC, Crossman SMA. 1999. Evaluation of tropical leaf vegetables in the virgin Islands. Janick J, ed. In Perspectives on New Crops and New Uses. ASHS Press, Alexandria, VA, USA. p 338-393.
  3. Wang KY. 1994. Chinese herbal medicine. Workman Press, Hong Kong. p 32.
  4. Lee KA, Lee SY, Woo SJ, Jo JS, Byun SM. 1992. Red pigments separated from flower of Korean cockscomb by agarose gel electrophoresis. Food Sci Biotechnol 1: 58-61
  5. Piattelli M, Impellizzeri G. 1970. 2-Descarboxybetanidin, a minor betacyanin from Carpobrotus acinaciformis. Phytochemistry 9: 2553-2556. https://doi.org/10.1016/S0031-9422(00)85776-3
  6. Minale L, Piattelli M, De Stefano S, Nicolaus RA. 1966. Pigments of centrospermae-VI. Acylated betacyanins. Phytochem 5: 1037-1052. https://doi.org/10.1016/S0031-9422(00)86098-7
  7. Nam HK, Rho GH. 1988. Composition of fatty acid and amino acid in water extracted material cockscomb plant root. J Korea Soc Food Nutr 17: 172-175.
  8. Sasaki N, Abe Y, Wada K, Koda T, Goda Y, Adachi T, Ozeki Y. 2005. Amaranthin in feather cockscombs is synthesized via glucuronylation at the cyclo-DOPA glucoside step in the betacyanin biosynthetic pathway. J Plant Res 118: 439-442. https://doi.org/10.1007/s10265-005-0237-z
  9. Wen Y, Islam MT, Tahata S. 2006. Phenolic constituents of Celosia cristata L. susceptible to spinach root rot pathogen Aphanomyces cochlioides. Biosci Biotechnol Biochem 70: 2567-2570. https://doi.org/10.1271/bbb.60272
  10. Ohno T, Inoue M, Ogihara Y, Saracoglu I. 2002. Atimetastatic activity of acteoside, a phenylethanoid glycoside. Biol Pharm Bull 25: 666-668. https://doi.org/10.1248/bpb.25.666
  11. Begam M, Narwal S, Roy S, Kumar S, Lodha ML, Kapoor HC. 2006. An antivivral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata. Biochemistry (Mosc) 71: S44-S48. https://doi.org/10.1134/S0006297906130074
  12. Kim JH, Yoon SJ, Lee KH, Kwon HJ, Chun SS, Kim TW, Cho YJ. 2005. Screening of biological activities of the extracts from Bisil (Ocimum basilicum L.). J Korean Soc Appl Biol Chem 48: 173-177.
  13. Dewanto V, Wu X, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964. https://doi.org/10.1021/jf0255937
  14. Duval B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativam) elicited by genetically transformed anise root extract. J Food Biochem 25: 361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  15. Sun B, Ricardo-da-Silva JM, Spranger I. 1998. Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 46: 4267-4274. https://doi.org/10.1021/jf980366j
  16. Nieva MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  17. Kim JE, Joo SI, Seo JH, Lee SP. 2009. Antioxidant and ${\alpha}$-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 38: 989-995. https://doi.org/10.3746/jkfn.2009.38.8.989
  18. Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci 2: 152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  19. Middleton E, Kandaswami C. 1994. Potential health-promoting properties of citrus flavonoids. Food Technol 48: 115-119.
  20. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632.
  21. Jorge AP, Horst H, Sousa E, Pizzolatti MG, Silva FR. 2004. Insulinomimetic effects of kaempferitrin on glycaemia and on 14C-glucose uptake in rat soleus muscle. Chem Biol Interact 149: 89-96. https://doi.org/10.1016/j.cbi.2004.07.001
  22. Escribano J, Pedreno MA, Garcia-Carmona F, Munoz R. 1998. Characterization of the antiradical activity of betalains from Beta vulgaris L. root. Phytochem Anal 9: 124-127. https://doi.org/10.1002/(SICI)1099-1565(199805/06)9:3<124::AID-PCA401>3.0.CO;2-0
  23. Kanner J, Harel S, Granit R. 2001. Betalains－a new class of dietary cationized antioxidants. J Agric Food Chem 49: 5178-5185. https://doi.org/10.1021/jf010456f
  24. Cai Y, Sun M, Corke H. 2003. Antioxidant activity of betalains from plants of the amaranthaceae. J Agric Food Chem 51: 2288-2294. https://doi.org/10.1021/jf030045u
  25. Jung WY, Jeong JM. 2012. Change of antioxidative activity at different harvest time and improvement of atopic dermatitis effects for persimmon leaf extract. Kor J Herbology 27: 41-49. https://doi.org/10.6116/kjh.2012.27.1.41
  26. de Sousa E, Zanatta L, Seifriz I, Creczynski-Pasa TB, Pizzolatti MG, Szpoganicz B, Silva FR. 2004. Hypoglycemic effect and antioxidant potential of kaempferol-3,7-O-(${\alpha}$)-dirhamnoside from Bauhinia forficata leaves. J Nat Prod 67: 829-832. https://doi.org/10.1021/np030513u
  27. Jeong KY, Kim ML. 2012. Physiological activities of Ulmus pumila K. extracts. Korean J Food Preserv 19: 104-109. https://doi.org/10.11002/kjfp.2012.19.1.104
  28. Jung SJ, Lee JH, Song HN, Seong NS, Lee SE, Baek NI. 2004. Screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem 47: 135-140.

Cited by

  1. Antioxidant and Anti-inflammatory Activities of Functional Plant Materials vol.23, pp.7, 2013, https://doi.org/10.5352/JLS.2013.23.7.869
  2. Antioxidant Activities of Amaranth (Amaranthus spp. L.) Flower Extracts vol.27, pp.2, 2014, https://doi.org/10.9799/ksfan.2014.27.2.175
  3. Antioxidant Activity of Solvent Fraction from Broccoli Sprouts Cultivated at the Plant Factory System vol.28, pp.1, 2015, https://doi.org/10.9799/ksfan.2015.28.1.001
  4. 아마란스 종자 추출물의 라디칼 저해활성 vol.18, pp.2, 2014, https://doi.org/10.13050/foodengprog.2014.18.2.116
  5. Anti-Oxidative and Anti-Diabetic Effects of Butanol Faction from Yangha (Zingiber mioga ROSC) vol.34, pp.1, 2018, https://doi.org/10.9724/kfcs.2018.34.1.105
  6. Quality Characteristics and Antioxidant Activities of Liquor Containing Rose, Camellia, and Cockscomb vol.28, pp.2, 2012, https://doi.org/10.17495/easdl.2018.4.28.2.101
  7. Phenolic Compounds and Antioxidant Activities of 21 Different Rose Flower Cultivar vol.50, pp.4, 2012, https://doi.org/10.3746/jkfn.2021.50.4.354