Journal of the Korean Ceramic Society (한국세라믹학회지)

The Korean Ceramic Society (한국세라믹학회)
권호 표지
DOI QR코드

DOI QR Code

Dependence of Crosslinking Temperature on Swelling Behavior of Hyaluronic Acid Porous Microbeads Synthesized by a Modified Spray Method

노즐 낙하법으로 제조한 히알루론산 다공성 마이크로비드의 가교온도에 따른 팽윤특성

  • Kim, Young-Hun (Department of Materials Engineering, Korea Aerospace University) ;
  • Lee, In-Kyu (Department of Materials Engineering, Korea Aerospace University) ;
  • Kim, Jin-Tae (Department of Materials Engineering, Daelim University) ;
  • Park, Ju-Hyun (Department of Materials Engineering, Daelim University) ;
  • Lee, Deuk Yong (Department of Materials Engineering, Daelim University)
  • 김영훈 (항공대학교 재료공학과) ;
  • 이인규 (항공대학교 재료공학과) ;
  • 김진태 (대림대학교 재료정보학과) ;
  • 박주현 (대림대학교 재료정보학과) ;
  • 이득용 (대림대학교 재료정보학과)
  • Received : 2012.06.29
  • Accepted : 2012.09.04
  • Published : 2012.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Hyaluronic acid (HA) microbeads were synthesized by dropping 0.5 wt% of sodium hyaluronate dissolved in NaOH into 0.2 vol% of divinyl sulfone dissolved in 2-methyl-1propanol at a speed of 0.005 ml/min. HA microbeads were collected from a divinyl sulfone crosslinker solution stirred at 200 to 400 rpm for 5 h at temperatures from room temperature to $60^{\circ}C$ at intervals of $10^{\circ}C$. The crosslinked microbeads were then cleaned thoroughly using distilled water and ethanol. SEM results revealed that the microbeads were white-colored spheres. The 3-D porous network structure of the microbeads became dense with an increase in the crosslinking temperature; however, no dependence of the crosslinking temperature on the microbead size was detected. The extent of swelling decreased from 970% to 670% with an increase in the crosslinking temperature from room temperature to $60^{\circ}C$, most likely due to the increase in the degree of crosslinking.

Keywords

References

  1. J.T. Kim and J.H. Choi, "Production and Evaluation of Hyaluronic Acid Gel for Soft Tissue Augmentation," Biomater. Res., 3 105-8 (2009).
  2. J.T. Kim, J.H. Choi, and D.Y. Lee, "Pyrogenicity of Hyaluronic Acid Hydrogel Cross-linked by Divinly Sulfone for Soft Tissue Augmentation,," Natural Sci., 2 764-68 (2010). https://doi.org/10.4236/ns.2010.27096
  3. J.T. Kim, D.Y. Lee, Y. Kim, I. Lee, and Y. Song, "Effect of pH on Swelling Behavior of Hyaluronic Acid Hydrogels for Smart Drug Delivery System," J. Sensor Sci. Technol., 21 256-57 (2012). https://doi.org/10.5369/JSST.2012.21.4.256
  4. A.P. Sclafani and T. Romo, "Collagen, Human Collagen and Fat: The Search for a Three-dimensional Soft Tissue Filler," Facial Plast. Surg., 17 79-85 (2011).
  5. J.Y. Kwon and S.I. Cheong, "Characterization of Hyaluronic Acid Membrane Cross Liked with Lactide," Polymer, 29 599-604 (2005).
  6. O. Jeon, S.J. Song, K.J. Lee, M.H. Park, S. Lee, S.K. Hahn, S. Kim, and B. Kim, "Mechanical Properties and Degradation Behaviors of Hyaluronic Acid Hydrogels Crosslinked at Various Cross-linking Densities," Carbohydrate Polymers, 70 251-57 (2007). https://doi.org/10.1016/j.carbpol.2007.04.002
  7. K. Kofuji, T. Ito, Y. Murata, and S. Kawashima, "The Controlled Release of a Drug from Biodegradable Chitosan Gel Beads," Chem. Pharm. Bull., 48 579-81(2000). https://doi.org/10.1248/cpb.48.579
  8. C. Mao and W.S. Kisaalita, "Characterization of 3-D Collagen Hydrogels for Functional Cell-based Biosensing," Biosen. Bioelect., 19 1075-88 (2004). https://doi.org/10.1016/j.bios.2003.10.008
  9. E.S. Song, S.Y. Kim, T. Chun, H. Byun, and Y.M. Lee, "Collagen Scaffolds Derived from a Marine Source and Their Biocompatibility," Biomater., 27 2951-61 (2006). https://doi.org/10.1016/j.biomaterials.2006.01.015
  10. N.J. Einerson, K.R. Stevens, and W.J. Kao, "Synthesis and Physiochemical Analysis of Gelatin-based Hydrogels for Drug Carrier Matrices," Biomater., 24 509-23 (2002).
  11. I. Kim, S. Seo, H. Moon, M. Yoo, I. Park, B. Kim, and C. Cho, "Chitosan and its Derivatives for Tissue Engineering Applications," Biotechnol. Advan., 26 1-21 (2008). https://doi.org/10.1016/j.biotechadv.2007.07.009
  12. J.Y. Kwon and S.I. Cheong, "Synthesis and Characterization of Hyaluronic Acid Bead Crosslinked by 1-3 Butadiene Diepoxide," Polymer, 29 445-50 (2005).
  13. J.T. Kim and J.H. Choi, "Preparation and Characterization of Hyaluronic Acid Microbeads," Biomater. Res., 14 157-60 (2010).
  14. A.K. Galwey and M.E. Brown, "Application of the Arrhenius Equation to Solid State Kinetics: Can This be Justified?," Thermochimica Acta, 386 91-8 (2002). https://doi.org/10.1016/S0040-6031(01)00769-9
  15. J.M. Rodrguez-Daz and M. Teresa Santos-Martn, "Study of the Best Designs for Modifications of the Arrhenius Equation," Chemometrics Intellig. Labor. Systems, 95 199-208 (2009). https://doi.org/10.1016/j.chemolab.2008.10.011
  16. L.J. Rodriguez-Araogna and J. Lopez-Fidalgo, "Optimal Designs for the Arrhenius Equation," Chemometrics Intellig. Labor. Systems, 77 131-38 (2005). https://doi.org/10.1016/j.chemolab.2004.06.007

Cited by

  1. Effect of molecular weight of hyaluronic acid (HA) on viscoelasticity and particle texturing feel of HA dermal biphasic fillers vol.20, pp.1, 2016, https://doi.org/10.1186/s40824-016-0073-3
  2. 3차원 다공성 콜라겐지지체의 제조 및 특성 분석 vol.35, pp.6, 2012, https://doi.org/10.9718/jber.2014.35.6.192
  3. 미세 유체 칩 기반의 히알루론산 미세 실의 제작 vol.38, pp.1, 2012, https://doi.org/10.9718/jber.2017.38.1.1