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Phylogenetic Analysis of New Isolates of Cucumber mosaic virus from Iran on the Basis of Different Genomic Regions

  • Received : 2012.06.10
  • Accepted : 2012.08.05
  • Published : 2012.12.01

Abstract

Molecular characterization of Cucumber mosaic virus (CMV) was done by using samples from tomato and cucurbitaceous plants collected from different locations in the northwest region of Iran. After screening by enzyme-linked immunosorbent assay, 91 CMV-infected samples were identified. Biological properties of eight representative isolates were compared with each other revealing two distinct phenotypes on squash and tomato plants. Phylogenetic analyses based on nucleotide sequences of the coat protein (CP), movement protein (MP) and 2b of the new isolates, together with that of previously reported isolates, led to the placement of the Iranian isolates in subgroups IA and IB according to CP and MP genes, but in subgroup IA according to the 2b gene. These data suggest that reassortment may have been a major event in the evolution of CMV in Iran, and that the Iranian isolates are derived from a common recent ancestor that had passed through a bottleneck event.

Keywords

References

  1. Bashir, N. S., Kolhar, M. R. and Zarghani, S. N. 2006. Detection, differentiation and phylogenetic analysis of cucumber mosaic virus isolates from cucurbits in the northwest region of Iran. Virus Genes 32:277-288. https://doi.org/10.1007/s11262-005-6912-2
  2. Bashir, N. S., Nematollahi, S. and Torabi, E. 2008. Cucumber mosaic virus subgroup IA frequently occurs in the northwest IRAN. Acta Virol. 52:237-242.
  3. Boccard, F. and Baulcombe, D. 1993. Mutational analysis of cisacting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 193:563-578. https://doi.org/10.1006/viro.1993.1165
  4. Bonnet, J., Fraile A, Sacristán, S., Malpica, J. M. and García-Arenal, F. 2005. Role of recombination in the evolution of natural populations of Cucumber mosaic virus, a tripartiteRNA plant virus. Virology 332:359-368. https://doi.org/10.1016/j.virol.2004.11.017
  5. Bujarski, J., Figlerowicz, M., Gallitelli, D., Roossinck, M. J. and Scott, S. W. 2012. Family Bromoviridae. In: Virus Taxonomy: Classification and Nomenclature of Viruses; Ninth Report of the International Committee on Taxonomy of Viruses, ed. by A. M. Q. King, M. J. Adams, E. B. Carstens and E. J. Lefkowitz, pp. 965-976. Elsevier Academic Press, Waltham, MA USA.
  6. Choi, S. K., Palukatis, P., Min, B. E., Lee, M. Y., Choi, J. K. and Ryu, K. H. 2005. Cucumber mosaic virus 2a polymerase and movement proteins independently affect both virus movement and the timing of symptom development in zucchini squash. J. Gen. Virol. 86:1213-1222. https://doi.org/10.1099/vir.0.80744-0
  7. Chung, C. T., Niemela, S. A. and Miller, R. H. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl Acad. Sci. USA 86:2172-2175. https://doi.org/10.1073/pnas.86.7.2172
  8. Clark, M. F. and Adams, A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483. https://doi.org/10.1099/0022-1317-34-3-475
  9. Ding, S. W., Anderson, B. J., Haase, H. R. and Symons, R. H. 1994. New overlapping gene encoded by the Cucumber mosaic virus genome. Virology 198:593-601. https://doi.org/10.1006/viro.1994.1071
  10. Du, Z. Y., Chen, F. F., Liao, Q. S., Zhang, H. R., Chen, Y. F. and Chen, J. S. 2007. 2b ORFs encoded by subgroup IB strains of Cucumber mosaic virus induce differential virulence on Nicotiana species. J. Gen. Virol. 88:2596-2604. https://doi.org/10.1099/vir.0.82927-0
  11. Felsenstein, J. 2004. PHYLIP (Phylogeny Inference Package) Version 3.65. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.
  12. Gallitelli, D. The ecology of Cucumber mosaic virus and sustainable agriculture. 2000. Virus Res. 71:9-21. https://doi.org/10.1016/S0168-1702(00)00184-2
  13. Hayes, R. J. and Buck, K. W. 1990. Complete replication of a eukaryoticvirus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368. https://doi.org/10.1016/0092-8674(90)90169-F
  14. Huppert, E., Szilassy, D., Salanki, K., Diveki, Z. and Balazs, E. 2002. Heterologous movement protein strongly modifies the infection phenotype of cucumber mosaic virus. J. Virol. 76:3554-3557. https://doi.org/10.1128/JVI.76.7.3554-3557.2002
  15. Lewsey, M. G., Surette, M., Robertson, M., Robertson, F. C., Ziebell, H., Choi, S. H., Ryu, K. H., Canto, T., Palukaitis, P., Payne, T., Walsh, J. A. and Carr, J. P. 2009. The Role of the Cucumber mosaic virus 2b Protein in Viral Movement and Symptom Induction. Mol. Plant-Microbe Interact. 22:642- 654. https://doi.org/10.1094/MPMI-22-6-0642
  16. Lewsey, M. G., Gonzalez, I., Kalinina, N. O., Palukaitis, P., Canto, T. and Carr, J. P. 2010. Symptom induction and RNA silencing suppression by the Cucumber mosaic virus 2b protein. Plant Signal. Behav. 5:705-708. https://doi.org/10.4161/psb.5.6.11643
  17. Li, H. W., Lucy, A. P., Guo, H. S., Li, W. X., Ji, L. H., Wong, S. M. and Ding, S. W. 1999. Strong host resistance targeted against a viral suppressor of the plant gene silencing defense mechanism. EMBO J. 18:2683-2691. https://doi.org/10.1093/emboj/18.10.2683
  18. Librado, P. and Rozas, J. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451-1452. https://doi.org/10.1093/bioinformatics/btp187
  19. Lin, H. Z., Rubio, L., Smythe, A. B. and Falk, B. W. 2004. Molecular population genetics of Cucumber mosaic virus in California: evidence for founder effects and reassortment. J. Virol. 78:6666-5575. https://doi.org/10.1128/JVI.78.12.6666-6675.2004
  20. Liu, Y. Y., Yu, S. L., Lan, Y. F., Zhang, C. L., Hou, S. S., Li, X. D., Chen, X. Z. and Zhu, X. P. 2009. Molecular variability of five Cucumber mosaic virus isolates from china. Acta Virol. 53:89-97. https://doi.org/10.4149/av_2009_02_89
  21. Nicholas, K. B., Nicholas, H. B. Jr. and Deerfield, D. W. II. 1997. GeneDoc: Analysis and Visualization of Genetic Variation, EMBNEW.NEWS 4:14.
  22. Page, R. D. M. 1996. Treeview - An application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 124:357-358.
  23. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348. https://doi.org/10.1016/S0065-3527(08)60039-1
  24. Palukaitis, P. and Zaitlin, M. 1997. Replicase-mediated resistance to plant virus disease. Adv. Virus Res. 48:349-377. https://doi.org/10.1016/S0065-3527(08)60292-4
  25. Palukaitis, P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv. Virus Res. 62: 241-323. https://doi.org/10.1016/S0065-3527(03)62005-1
  26. Perry, K. L., Zhang, L., Shintaku, M. H. and Palukaitis, P. 1994. Mapping determinants in Cucumber mosaic virus for transmission by Aphis gossypii. Virology 205:591-595. https://doi.org/10.1006/viro.1994.1686
  27. Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, R. M. 1992. Differentiation of Cucumber mosaic virus isolates using the polymerase chain reaction. J. Gen. Virol. 73:2099-2103. https://doi.org/10.1099/0022-1317-73-8-2099
  28. Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangements in the 5' nontranslated region and phylogenetic analyzes of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73:6752-6758.
  29. Roossinck, M. J. 2001. Cucumber mosaic virus as a model for RNA virus evolution. Mol. Plant Pathol. 2:59-63. https://doi.org/10.1046/j.1364-3703.2001.00058.x
  30. Roossinck, M. J. 2002. Evolutionary history of Cucumber mosaic virus deduced by phylogenetic analyses. J. Virol. 76:3382- 3387. https://doi.org/10.1128/JVI.76.7.3382-3387.2002
  31. Rowhani, A., Chay, C., Golino, D. A. and Falk, W. 1993. Development of a polymerase chain reaction technique for the detection of Grapevine fanleaf virus in a grapevine tissue. Phytopathology 83:749-753. https://doi.org/10.1094/Phyto-83-749
  32. Rychlik, W. 1994. Oligo Primer Analysis Software version 5.0. Published by National Biosciences Inc., MN, USA.
  33. Shi, B. J., Palukaitis, P. and Symons, R. H. 2002. Differential virulence by strains of Cucumber mosaic virus is mediated by the 2b gene. Mol. Plant-Microbe Interact. 15:947-955. https://doi.org/10.1094/MPMI.2002.15.9.947
  34. Shintaku, M. H., Zhang, L. and Palukaitis, P. 1992. A single amino acid substitution in the coat protein of Cucumber mosaic virus induces chlorosis in tobacco. Plant Cell 4:751- 757. https://doi.org/10.1105/tpc.4.7.751
  35. Soards, A. J., Murphy, A. M., Palukaitis, P. and Carr., J. P. 2002.Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol. Plant-Microbe Interact. 15:647-653. https://doi.org/10.1094/MPMI.2002.15.7.647
  36. Sugiyama, M., Sato, H., Karasawa, A, Hase, S., Takahashi, H. and Ehara, Y. 2002. Characterization of symptom determinants in two mutants of Cucumber mosaic virus Y strain, causing distinct mild green mosaic symptoms in tobacco. Physiol. Mol. Plant Pathol. 56:85-90.
  37. Suzuki, M., Kuwata, S., Masuta, C. and Takanami, Y. 1995. Point mutations in the coat protein of Cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J. Gen. Virol. 76:1791-1719. https://doi.org/10.1099/0022-1317-76-7-1791
  38. Van de Peer, Y. and De Wachter, R. 1997. Construction of evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites. Comput. Appl. Biosci. 13:227-230.

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