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Isolation and Physiological Characterization of a New Algicidal Virus Infecting the Harmful Dinoflagellate Heterocapsa pygmaea

  • Kim, JinJoo (Department of Fisheries Biology, Pukyong National University) ;
  • Kim, Chang-Hoon (Department of Fisheries Biology, Pukyong National University) ;
  • Takano, Yoshihito (Department of Environmental Science and Engineering, Yamaguchi University) ;
  • Jang, In-Kwon (National Fisheries Research & Development Institute) ;
  • Kim, Si Wouk (Department of Environmental Engineering, Chosun University) ;
  • Choi, Tae-Jin (Department of Microbiology, Pukyong National University)
  • Received : 2012.07.04
  • Accepted : 2012.07.30
  • Published : 2012.12.01
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Abstract

Dinoflagellates are considered one of the most abundant and diverse groups of marine microplankton and viruses are recognized as one of the significant factors affecting the plankton dynamics. Here, we report basic characteristics of a new dinoflagellate-infecting virus, Heterocapsa pygmaea DNA virus (HpygDNAV) which infects a toxic dinoflagellate, H. pygmaea. HpygDNAV is a polyhedral large virus (ca. 160-170 nm in diameter) propagating in its host's cytoplasm. Because of the virion size, appearance in thin sections, and propagation characteristics, HpygDNAV is assumed to harbor a large double-stranded DNA genome; i.e., HpygDNAV is most likely a nucleocytoplasmic large DNA virus (NCLDV) belonging to the family Phycodnaviridae. Its infectivity is strain-specific, rather than species-specific, as is the case for other algal viruses. The burst size and latent period are estimated to be roughly 100-250 infectious units $cell^{-1}$ and < 96 h, respectively.

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References

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