Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
권호 표지

Development of an Escherichia coli Biofilm Model on Transwell®

  • Kim, Bok Yung (Department of Biomedical Laboratory Science, Dongseo University) ;
  • Thyiam, General (Department of Biotechnology, Dongseo University) ;
  • Kang, Ji-Eun (Department of Biomedical Laboratory Science, Dongseo University) ;
  • Lee, Seung-Hwan (Department of Biomedical Laboratory Science, Dongseo University) ;
  • Park, Sang-Hee (Department of Biotechnology, Dongseo University) ;
  • Kim, Jung-Sun (Department of Biomedical Laboratory Science, Dongseo University) ;
  • Abraham, Marion (Department of Biomedical Laboratory Science, Dongseo University)
  • Received : 2012.08.30
  • Accepted : 2012.09.19
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Escherichia coli biofilm, reported to be produced in the human intestine causing a significant health risk, was successfully grown on transwell$^{(R)}$. This biofilm layer was identified by crystal violet staining and prepared for the in vitro E. coli biofilm system which can be used to screen for inhibitors. The biofilm formation did not show a change in transepithelial electrical resistance values. Furthermore, rhodamine 123 staining showed that the dye did not pass through the membrane once biofilm was formed.

Keywords