Asian-Australasian Journal of Animal Sciences

  • 제25권1호
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  • Pages.114-121
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  • 2012
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability

  • Upadhaya, Santi Devi (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Song, Jae-Yong (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Park, Min-Ah (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Seo, Ja-Kyeom (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Yang, Liu (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University) ;
  • Lee, Chan-Ho (Genebiotech co., Ltd.) ;
  • Cho, Kyung-J. (Genebiotech co., Ltd.) ;
  • Ha, Jong-K. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Science, Seoul National University)
  • 투고 : 2011.04.14
  • 심사 : 2011.11.18
  • 발행 : 2012.01.01
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초록

The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at $39^{\circ}C$. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid 'corn' substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications.

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참고문헌

  1. Abrunhosa, L., R. Serra and A. Venancio. 2002. Biodegradation of ochratoxin A by fungi isolated from grapes. J. Agric. Food Chem. 50:7493-7496. https://doi.org/10.1021/jf025747i
  2. Bata, A. and R. Lasztity.1999. Detoxification of mycotoxin-contaminated food and feed by micro-organisms. Trends Food Sci. Technol. 10:223-228. https://doi.org/10.1016/S0924-2244(99)00050-3
  3. Ciegler, A., E. B. Lillehoj, R. E. Peterson and H. H. Hall. 1966. Microbial detoxification of Aflatoxin. Appl. Microbiol. 14:934-939.
  4. Deberghes, P., A. M. Betbeder, F. Boisard, R. Blanc, J. F. Delaby, S. Krivobok, R. Steiman, F. Seigle-Murandi and E. E. Creppy. 1995. Detoxification of ochratoxin A, a food contaminant: Prevention of growth of Aspergillus ochraceus and its production of ochratoxin A. Mycotoxin Res. 11:37-47. https://doi.org/10.1007/BF03192060
  5. Doelle, H. W., D. A. Mitchell and C. E. Rolz. 1992. Solid State Fermentation, Elsevier, London, 1992.
  6. Fuchs, S., G. Sontag, R. Stidl, V. Ehrlich, M. Kundi and S. Knasmuller. 2008. Detoxification of patulin and ochratoxin A, two abundant mycotoxins, by lactic acid bacteria. Food Chem. Toxicol. 46:1398-1407. https://doi.org/10.1016/j.fct.2007.10.008
  7. Food and Agriculture Organization of the United Nations. 1996. Basic facts of the world cereal situation. Food Outlook 1996, 5/6.
  8. Galtier, P. and M. Alvinerie. 1976. In vitro transformation of ochratoxin A by animal microbial floras. Ann. Rech. Vet. 7: 91-98.
  9. Hult, K., A. Teiling and S. Gatenbeck. 1976. Degradation of ochratoxin A by a ruminant. Appl. Environ. Microbiol. 32: 443-444.
  10. Madhyastha, M. S., R. R. Marquardt and A. A. Frohlich. 1992. Hydrolysis of ochratoxin A by microbial activity of digesta in the gastrointestinal tract of rats. Arch. Environ. Contam. Toxicol. 23:468-472.
  11. Peteri, Z., J. Teren, C. Vegvolgyi and J. Varga. 2007. Ochratoxin degradation and adsorption caused by astaxanthin-producing yeasts. Food Microbiol. 24:205-210. https://doi.org/10.1016/j.fm.2006.06.003
  12. Rambaut A. 2002. Se-Al sequence element editor version 2.0a11. University of Oxford, Oxford UK.
  13. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  14. Smith, J. E. and M. O. Moss. 1985. Mycotoxins. Formation, Analysis and Significance, Wiley, Chichester.
  15. Stander, M. A., U. T. Bornscheuer, E. Henke and P. S. Steyn. 2000. Screening of commercial hydrolases for the degradation of ochratoxin A. J. Agric. Food Chem. 48:5736-5739. https://doi.org/10.1021/jf000413j
  16. Salanitro, J. P., I. G. Fairchilds and Y. D. Zgornicki. 1974. Isolation, culture characteristics and identification of anaerobic bacteria from the chicken cecum. Appl. Microbiol. 27:678-687.
  17. SAS Inst. Inc. 2002. SAS user's guide version 9.1. SAS Inst. Inc. Cary, NC, USA.
  18. Schatzmayr, G., F. Zehner, M. Taubel, D. Schatzmayr, A. Klimitsch, A. P. Loibner and E. M. Binder. 2006. Microbiologicals for deactivating mycotoxins. Mol. Nutr. Food Res. 50:543-551. https://doi.org/10.1002/mnfr.200500181
  19. Scott, P. M. 1991. In: Cereal Grain Mycotoxins, Fungi and Quality in Drying and Storage (Ed. J. Chelkowski), Vol. 26, Elsevier, Amsterdam, pp. 529-572.
  20. Scott, P. M. 1996. Effects of processing and detoxification treatments on ochratoxin A: introduction. Food Addit. Contam. 13(Supp. l):19-21.
  21. Sweeney, M. J. and A. D. W. Dobson. 1998. Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int. J. Food Microbiol. 43:141-158. https://doi.org/10.1016/S0168-1605(98)00112-3
  22. Upadhaya, S. D., M. A. Park and Jong K. Ha. 2010. Mycotoxins and their biotransformation in the rumen: A review. Asian-Aust. J. Anim. Sci. 23(9):1250-1260. https://doi.org/10.5713/ajas.2010.r.06
  23. Upadhaya, S. D., Y. Liu, J. K. Seo, M. H. Kim, C. H. Lee, C. K. Lee and Jong K. Ha. 2011. Effect of feed types on Ochratoxin A disappearance in goat rumen fluid. Asian-Aust. J. Anim. Sci. 24(2):198-205. https://doi.org/10.5713/ajas.2011.10318
  24. Varga, J. and B. Toth. 2004. Strategies to control mycotoxins in feeds. Acta Vet. Hung. 53:189-203.
  25. Varga, J., Z. Peteri, K. Tabori, J. Teren, J. Vagvo and C. lgyi. 2005. Degradation of ochratoxin A and other mycotoxins by Rhizopus isolates. Int. J. Food Microbiol. 99:321-328. https://doi.org/10.1016/j.ijfoodmicro.2004.10.034
  26. Varga, J., K. Rigo and J. Teren. 2000. Degradation of ochratoxin A by Aspergillus species. Int. J. Food Microbiol. 59:1-7. https://doi.org/10.1016/S0168-1605(00)00230-0
  27. Wegst, W. and F. Lingens. 1983. Bacterial degradation of ochratoxin A. FEMS Microbiol. Lett. 17:341-344. https://doi.org/10.1111/j.1574-6968.1983.tb00433.x
  28. Xiao, H., R. R. Marquardt, A. A. Frohlich, G. D. Phillips and T. G. Vitti. 1991. Effect of hay and a grain diet on the rate of hydrolysis of ochratoxin A in the rumen of sheep. J. Anim. Sci. 69:3706-3714.

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  5. Advances in Biodetoxification of Ochratoxin A-A Review of the Past Five Decades vol.9, pp.1664-302X, 2018, https://doi.org/10.3389/fmicb.2018.01386
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