Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Anti-Microbial and Anti-Wrinkle Effect of Kaempferol and Kaempferol Rhamnosides isolated from Hibiscus cannabinus L.

양마에서 분리한 Kaempferol과 그 배당체의 항균 및 주름억제 효과

  • 이근하 ((주) 모아캠 기술연구소) ;
  • 공혜진 ((주) 모아캠 기술연구소) ;
  • 조영롱 ((주) 모아캠 기술연구소) ;
  • 주철규 ((주) 모아캠 기술연구소) ;
  • 권순상 ((주) 모아캠 기술연구소) ;
  • 황재성 (경희대학교 피부생명공학센터) ;
  • 박청 ((주) 모아캠 기술연구소)
  • Received : 2012.09.12
  • Accepted : 2012.11.26
  • Published : 2012.12.30
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Abstract

In this study, kaempferol and its rhamnosides (${\alpha}$-rhamnoisorobin, afzelin, kaempferitrin) were isolated electively by bioconversion technology from Hibiscus cannabinus L. leaves to evaluate the anti-wrinkles effects and anti-microbial effects. In order to evaluate anti-wrinkles activity, reduction of expression matrix metalloproteinase-1 (MMP-1) protein and proliferation/ pro-collagen production were investigated. Kaempferol and ${\alpha}$-rhamnoisorobin showed inhibition activity of MMP-1 generated to compared to positive control. In HaCaT cell proliferation assay, kaempferol and ${\alpha}$-rhamnoisorobin significantly promoted cell proliferation in a concentration-dependent manner. In addition, procollagen synthesis assays (by HDF-N cell) showd that TGF-${\beta}$ induced procollagen production and also, all four kinds of experimental significantly promoted procollagen synthesis in a concentration-dependent manner. Kaempferol and ${\alpha}$-rhamnoisorobin exhibited strong antimicrobial activities on five of microbes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger.

Keywords

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