The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effects of Medium, Temperature and pH on Mycelial Growth and Cellulase Activity of Ectomycorrhizal Fungi from Korean Forests

우리나라 산림에서 분리한 외생균근균의 균사생장에 있어 배지, 온도, pH의 영향과 셀룰라아제 활성

  • Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Kim, Min-Soo (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 전성민 (국립산림과학원 화학미생물과) ;
  • 김민수 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2012.11.17
  • Accepted : 2012.12.06
  • Published : 2012.12.31
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Abstract

Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran's agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma matsutake. Mycelial growth on PDA was poor at high temperature ($30^{\circ}C$) than the low temperature ($10^{\circ}C$). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were $20-25^{\circ}C$ and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and the maximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.

우리나라 산림에서 수집한 외생균근균 8종 27개 균주에 대한 균사 생장 특성을 다양한 배양 조건(배지, 온도, pH)에서 조사하였다. 60일 배양 후, 모든 균주는 PDA와 MMN 배지에서 생장하였으나, 송이를 포함한 일부 균주들은 MEA나 SDA 배지에서 전혀 생장하지 않았다. 또한 대부분의 균주들을 PDA 배지에서 배양했을 때에 저온($10^{\circ}C$)보다 고온($30^{\circ}C$)에서 균사 생장이 저조하였다. 또한 PDA 배지 상에서의 최적 생장온도는 $20-25^{\circ}C$, PDB 배지 내 균사 생장을 위한 최적 pH는 4-5로 나타났다. Carboxymethyl cellulose(CMC) 활성은 모든 시험 균주에서 나타났으며, CMC(pH 5.0) 한천배지 상에서 최대의 셀룰라아제 활성을 나타낸 균주는 송이(1266)였다.

Keywords

References

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