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된장에서 분리한 효모(Pichia farinosa NASS-2)의 돈분 악취감소효과

Decrease efficiency of Offensive Odor from Pig Excreta by Yeast Strain, Pichia farinosa NASS-2 Isolated from Soy Bean Paste

  • Yoo, Jae Hong (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, In Cheol (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Wan Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2012.11.12
  • 심사 : 2012.12.10
  • 발행 : 2012.12.31

초록

경기도 지방의 된장 시료로부터 악취감소 효과 미생물 중에서 효모가 분리되었으며 rDNA염기서열분석에 의하여 Pichia farinosa로 동정 되었다. 공시균주 P. farinosa NASS-2로 대량배양조건 확립 및 미생물처리제를 제조하였다. 분리한 효모로 제조한 악취저감효과 처리제의 실내 시험결과 돈분에 미생물제를 처리하고 15일 경과 후 악취가스 감소율을 조사한 결과, 암모니아가스($NH_3$ gas)는 처리 전 19.49 ppm에서 처리 후는 1.38 ppm으로, 황화수소가스($H_2S$ gas)는 처리 전 5.89 ppb에서 처리 후는 0.32 ppb으로 감소하는 효과를 나타내었다. 일반적으로 박테리아가 악취저감효과 미생물로 사용되고 있으나 본 연구에서 사용한 된장에서 분리한 효모 특히 산막효모인 P. farinosa NASS-2의 악취효과(암모니아가스, 황화수소 가스)는 처음 보고이다.

The different microbial species were isolated from soy bean paste samples. A yeast strain NASS-2 was effective to decrease odor activity on pig excreata was identified as Pichia farinosa based on nucleotide sequences of 18S ribosomal DNA and Internal transcribed space (ITS). The extracellular fraction of P. farinosa NASS-2 was effective to decrease odor activity of pig excrements. Optimal medium component for decreasing order activity on odor material composed of soluble starch 2.0% (w/v) and yeast extract 0.8% (v/v). The decrease of odor material was maximum at $28^{\circ}C$ for 72 hours with pH 5.5. When the P. farinosa NASS-2 culture broth was treated to pig excrements, the removal efficiency was an average concentration with 1.38 ppm of ammonia gas.

키워드

참고문헌

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피인용 문헌

  1. Study on the reduction of livestock malodor using microbial agents- focusing on swine facilities vol.17, pp.2, 2018, https://doi.org/10.15250/joie.2018.17.2.85