Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Evaluation of Mucosal Immunity in BTBR T+tf/J Mice Resembling Autism Spectrum Disorder

자폐증 유사증상 발현 마우스의 점액 면역에 대한 연구

  • Hwang, So Ryeon (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Jo, Ji Hoon (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Shin, Kyeong Min (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Jang, Yun Young (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Kim, Ji Youn (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Yeo, Kyeong Uk (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health) ;
  • Kim, Hyoung Ah (The Catholic University of Korea, College of Medicine, Dept. Preventive Medicine) ;
  • Heo, Yong (Catholic University of Daegu, College of Natural Sciences, Dept. Occupational Health)
  • 황소련 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 조지훈 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 신경민 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 장윤영 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 김지연 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 여경욱 (대구가톨릭대학교 자연대학 산업보건학과) ;
  • 김형아 (가톨릭대학교 의과대학 예방의학교실) ;
  • 허용 (대구가톨릭대학교 자연대학 산업보건학과)
  • Received : 2012.11.05
  • Accepted : 2012.11.28
  • Published : 2012.12.31
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Abstract

Objectives: This study was undertaken in order to evaluate a potential mechanism involved in gastro-intestinal problems observed in autistic subjects and uses an animal model of autism investigation. Methods: BTBR T+tf/J, a mouse strain with typical socio-behavioral characteristics of autistic subjects and FVB mice with highly social behaviors as the control strain were used. Both genders of mice aged three weeks and six months were used from four separate litters for each strain. Serum was prepared following cardiac puncture, and mesenteric lymph nodes were collected for in vitro stimulation and enumeration of major immune cell proportion. Results: The level of serum IgA was significantly enhanced in six-month-old BTBR mice compared with three-week-old BTBR, which was not observed with the FVB control mice. The serum IgE level was also higher among BTBR mice than among age-sex matched FVB mice, respectively. Considering the ratio of interleukin-4 vs interferon-gamma production from mesenteric lymph node T cells, skewedness toward type-2 reactivities was observed. In addition, the proportion of B cells in mesenteric lymph nodes was significantly higher in BTBR mice than in FVB mice. Conclusion: Upregulation of mucosal immunity related with enhanced type-2 immune reactivity observed in BTBR mice could be involved with the etiology of gastro-intestinal abnormalities in autism.

Keywords

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