Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Comparison of Melanogenesis-Inhibiting Activity by Extracts of Prunus persica Flower and Calyx

도화 및 도화악 추출물의 미백활성 비교

  • Son, Hyeong-U (Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Suk Hee (Cheongdo Peach Experiment Station) ;
  • Kim, Min-A (Department of Food Science & Technology, Kyungpook National University) ;
  • Park, Hee-Joon (CA Pharm Co, Ltd.) ;
  • Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
  • 손형우 (경북대학교 식품공학부) ;
  • 이숙희 (경북농업기술원 청도복숭아시험장) ;
  • 김민아 (경북대학교 식품공학부) ;
  • 박희준 ((주)씨에이팜) ;
  • 이상한 (경북대학교 식품공학부)
  • Received : 2012.09.06
  • Accepted : 2012.12.05
  • Published : 2012.12.30
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Abstract

The antioxidant activity and whitening effect of the distilled water (DW) and ethanol extracts of the Prunus persica flower and calyx were studied. In the oxygen radical absorption capacity (ORAC) assay for antioxidant activity measurement, it was confirmed that the flower extract was stronger than the calyx extract, and that the ethanol extract was relatively stronger than the DW extract. To define the whitening effect, an experiment was conducted involving tyrosinase inhibitory assay and measurement of the melanin content of B16F10. As a result of the use of tyrosinase, the DW extract of calyx showed 53% inhibition as the highest activity. The melanin content inhibitory rates were defined as 57% for the ethanol flower extract and 63% of the ethanol calyx extract, based on a $10{\mu}g/mL$ concentration. Based on these results, mixture with the whitening effect in the extract of P. persica and another compounds should be researched for development as a cosmetic ingredient.

복숭아꽃(도화)과 꽃받침(도화악)의 DW 및 에탄올 추출물로부터 미백활성을 측정하였다. ORAC assay에서 공통적으로 복숭아꽃 추출물이 꽃받침에 비해 높은 활성을, 에탄올 추출물이 DW 추출물 보다 상대적으로 활성이 높은 것을 확인하였다. Tyrosinase에 의한 미백활성을 비교하여 본 결과 10 mg/mL 농도를 기준으로 복숭아꽃 DW 추출물: 40%, 에탄올 추출물: 32%, 복숭아꽃받침 DW 추출물: 53%, 에탄올 추출물: 43%의 저해율로 복숭아꽃받침 DW 추출물에서 가장 높은 활성을 나타내었다. B16F10 세포에서 melanin 생합성 억제를 측정한 결과, 복숭아 꽃 에탄올 추출물의 $10{\mu}g/mL$에서 57%, $100{\mu}g/mL$에서 76%의 억제 효과를, 복숭아 꽃받침 에탄올 추출물의 $10{\mu}g/mL$에서 63%의 억제 효과를 확인하였다. 이 결과를 토대로 복숭아꽃 및 꽃받침의 물 또는 에탄올 추출물은 다른 천연성분과 적절히 혼합 사용 시 우수한 시너지효과를 나타낼 수 있는 소재임을 확인하였다.

Keywords

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