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Snowdrop lectin (Galanthus nivalis agglutinin: GNA) 유전자 도입에 의한 벼멸구 저항성 형질전환 벼 개발

Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin; GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stål)

  • 이수인 (국립농업과학원 농업생명자원부) ;
  • 윤인선 (국립농업과학원 농업생명자원부) ;
  • 김진아 (국립농업과학원 농업생명자원부) ;
  • 홍준기 (국립농업과학원 농업생명자원부) ;
  • 박범석 (국립농업과학원 농업생명자원부) ;
  • 이연희 (국립농업과학원 농업생명자원부)
  • Lee, Soo In (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA) ;
  • Yoon, In Sun (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA) ;
  • Kim, Jin A (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA) ;
  • Hong, Joon Ki (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA) ;
  • Park, Beom-Seok (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA) ;
  • Lee, Yeon-Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA)
  • 투고 : 2012.11.12
  • 심사 : 2012.11.16
  • 발행 : 2012.12.30

초록

유전자총(Particle bombardment) 방법에 의해 snowdrop lectin (Galanthus nivalis agglutinin; GNA) 유전자가 도입된 해충저항성 형질전환 벼를 개발하였다. GNA 유전자가 도입된 많은 형질전환 식물체가 재분화되었으며, 형질전환 벼의 GNA 유전자의 integration, expression 및 inheritance는 Southern 및 western analysis분석 방법에 의해 증명되었다. 벼 genome 내 도입 유전자 수는 one 혹은 five copies이었다. 형질전환 $R_1$$R_2$ 식물체의 GNA 단백질 발현 분석 결과, 함량은 총 단백질 중 0.01%부터 2.0%까지 포함하고 있었다. 형질전환 식물체 중 GNA 과발현 형질전환 벼를 이용한 생물 검정 결과, 대조구와 비교하여 벼의 주요 해충인 벼멸구(Nilaparvata lugens St${\aa}$l)에 저항성을 나타내었다. 위의 실험 결과를 통해, 해충저항성 유전자인 GNA가 도입된 형질전환 벼는 해충의 증식을 억제하는데 이용될 수 있을 것이다.

Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens St${\aa}$l) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated. The integration, expression, and inheritance of this gene in the $R_1$ and $R_2$ generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the $R_1$ and $R_2$ transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the $R_2$ transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA into rice plants therefore can help to control insect pests.

키워드

참고문헌

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