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Antioxidant Activity and Fermentation Characteristics of Traditional Black Rice Wine

흑미 첨가 막걸리의 항산화 및 발효 특성

  • Received : 2012.08.29
  • Accepted : 2012.09.25
  • Published : 2012.12.31

Abstract

In this study we examined the antioxidant activity and fermentation characteristics of black rice wine. The antioxidant activity of black rice wine was higher than the control, and DPPH radical scavenging activity showed a correlation with the anthocyanin content of rice wine. The pH remarkably decreased until 3 days of fermentation, and the gradually decreased. The reducing sugar and free sugar content reached a maximum at 1 day of fermentation due to enzyme activity. The amount of organic acids, especially lactic acid, increased during the fermentation period. Lactic acid bacteria and yeast count increased with time and amount of black rice. The L color value increased during fermentation regardless of treatments, and the a color value increased with ratio of black rice due to anthocyanin. For this reason, color was given a high score in black rice wine. But overall preference was high in rice wine made with less than 20% of black rice.

본 연구는 흑미를 첨가하여 막걸리를 제조하여 항산화 및 발효특성을 살펴보았다. 항산화 활성은 대조구에 비해 흑미 첨가구가 높았으며 특히 DPPH 라디칼 소거능은 안토시아닌 함량과 비례하였다. 발효 3일째, pH는 급격히 감소하였으며 대조구에 비해 흑미 첨가구가 낮았다. 환원당과 유리당은 1일째 급격히 증가하였다가 그 이후 감소하는 경향을 나타내었는데 이는 발효 초기에 당화 효소 활성으로 쌀의 전분이 glucose와 maltose 등으로 분해되기 때문이다. 모든 유기산은 발효 동안 증가하였으며 lactic acid가 가장 높은 증가율을 나타내었다. 발효 동안 흑미 첨가 막걸리의 lactic acid와 succinic acid는 대조구와 큰 차이를 보이지 않았다. 발효 초기 젖산균수가 증가하여 3일째 최대에 달하였으며 이는 lactic acid가 급증한 시기와 일치하였다. 또한 흑미 첨가량이 많을수록 젖산균수가 높았다. 색도는 흑미의 붉은색의 영향으로 대조구에 비해 흑미 첨가구의 a값이 높았다. 관능검사 결과 외관은 흑미 첨가구가 높은 점수를 받았으나 전체적으로 단맛과 신맛의 영향을 받아 대조구와 20% 첨가구를 선호하는 것으로 나타났다. 흑미를 첨가할수록 항산화 성분은 증가하나 관능평가 결과로 보았을 때 흑미는 20% 수준 첨가하는 것이 바람직한 것으로 나타났다.

Keywords

References

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