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Lipolytic Effect of Sparassis crispa Extracts in Differentiated 3T3-L1 Cells and High Fat Diet-induced Obese Mice

분화된 3T3-L1 세포와 비만유도 쥐에 꽃송이버섯 추출물의 지질분해 효과

  • Lee, Mi-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Jin-Kyung (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Um, Mi-Hyang (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Jeon, Jung-Woo (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Jung-Min (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Yoo-Kyoung (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 이미애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박진경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 엄미향 (경희대학교 동서의학대학원 의학영양학과) ;
  • 전정우 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이정민 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박유경 (경희대학교 동서의학대학원 의학영양학과)
  • Received : 2012.08.09
  • Accepted : 2012.11.28
  • Published : 2012.12.31

Abstract

The purpose of this study was to investigate whether water extracts of Sparassis crispa (SC) have anti-obesity effects. Treatment of mature adipocytes with SC caused a decrease in lipid accumulation (assessed by Oil Red O staining) and an increase in glycerol release. Mice were induced to obesity by a high fat diet (45% fat in total kcal) and experimental groups were treated with two different dosages of SC extracts, a low SC (LSC, 100 mg/kg/day) or high SC (HSC, 300 mg/kg/day). SC extracts were administered by gavages for 10 weeks in the experimental groups, while the control group was fed with distilled water. The body weight gain of mice fed SC was significantly reduced (11.88% lower in LSC, 14.54% lower in HSC) compared to the control group. Additionally, there were significantly reduced serum levels of triglycerides (13.57% lower in LSC, 19.46% lower in HSC), total cholesterol (32.22% lower in LSC, 24.67% lower in HSC) and glucose (28.85% lower in LSC, 25.82% lower in HSC) in mice fed SC compared to the control group. Hepatic triglycerides in mice fed SC were lower (9.68% lower in LSC, 14.24% lower in HSC) than the control group and total cholesterol levels were also lower in mice fed SC (38.72% lower in LSC, 35.20% in HSC). These results demonstrate that the water extract of SC may enhance lipolysis and up-regulate the expression of lipolytic enzymes in vitro and reduce body weight in vivo. These significant effects were found for both low and high doses of SC treatment, and suggest SC can be used as potential therapeutic substances for the prevention and treatment of obesity.

본 연구에서는 꽃송이버섯 추출물을 처리하여 분화된 3T3-L1 지방 세포와 비만 유도 쥐에서 지질분해 효과를 살펴보았다. In vitro, preadipocyte에서 MTT assay를 이용하여 세포독성 실험을 한 결과 시간, 농도에 관계없이 세포생존율에 영향을 미치지 않았고, 분화된 지방세포에서의 지방분해 효과는 농도 의존적으로 감소되는 것을 확인할 수 있다. 이외에도 지방세포에서 지방분해 산물인 glycerol 양을 측정한 결과 48, 72시간에서 시간, 농도 의존적으로 유의성 있게 증가하였다. Lipolytic enzyme의 CPT-1 및 UCP-2 발현을 측정하기 위해 Western blot assay를 실시한 결과 꽃송이버섯 열수추출물이 CPT-1 및 UCP-2의 발현을 증가시키는 것을 확인할 수 있었다. In vivo에서 고지방 식이에 의해 비만이 유도된 쥐에 꽃송이버섯 열수추출물을 처리한 후 실험 종료 시 실험동물의 체중은 고지방식이를 공급한 군에 비해 저농도, 고농도 꽃송이버섯을 처리한 군 각각 유의적으로 감소하였다. 실험동물의 장기와 지방조직 무게를 측정한 결과, 대조군에 비해 추출물을 처리한 군에서 체중과 장기 및 지방조직의 감소가 저농도, 고농도 모두 유의하게 나타났으며, 혈청 및 간의 지질함량을 측정한 결과, 혈청지질의 중성지방, 총 콜레스테롤 함량 모두 대조군에 비해 유의적으로 낮게 나타났으며, HDL-콜레스테롤 함량은 유의적으로 높게 나타났다. 간의 지질함량을 측정한 결과 중성지방은 유의적으로 낮게 나타났고 총 콜레스테롤 역시 대조군에 비해 유의적으로 낮게 나타났다. 따라서 혈청 및 간의 지질함량을 측정한 결과 꽃송이버섯 열수추출물이 비만이 유도된 쥐의 혈청 및 간의 지질 농도 변화를 낮추는 효과가 있다고 사료된다. 따라서 꽃송이버섯 열수추출물은 lipolytic enzyme의 발현을 증가시켜 지방산의 산화 반응 및 열 발생에 의한 에너지 소비율을 증가시켜 lipid accumulation을 감소시키며, 이로 인한 체중 감소 및 혈청이나 간의 지질함량을 개선시켜 지질분해 효과를 보여줌으로써 비만 예방 및 치료를 목적으로 하는 식품 소재로서의 활용이 가능할 것으로 생각된다.

Keywords

References

  1. Kopelman PG. 2000. Obesity as a medical problem. Nature 404: 635-643. https://doi.org/10.1038/35007508
  2. Chon JW, Park JK, Lee MA, Jeong MR, Han JH, Park YK. 2009. Fermented crataegi fructus vinegar improves lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 38: 1024-1031. https://doi.org/10.3746/jkfn.2009.38.8.1024
  3. Cho YS, Jang EM, Jang SM, Chun MS, Shon MY, Kim MJ, Lee MK. 2007. Effect of grape seed water extract on lipid metabolism and erythrocyte antioxidant defense system in high-fat diet-induced obese C57BL/6 mice. J Korean Soc Food Sci Nutr 36: 1537-1543. https://doi.org/10.3746/jkfn.2007.36.12.1537
  4. Kim BK, Shin GK, J eon BS, Bae DW, Cha JY. 2001. Cholesterol lowering effect of mushroom power in hyperlipidemic rats. J Korean Soc Food Sci Nutr 30: 510-515.
  5. Yotsumoto H, Yanagita T, Yotsumoto K, Ogawa Y, Cha JY, Mori Y. 1997. Inhibitory effect of oren-gedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells: evidence from the cultured HepG2 cells and in vitro assay of ACAT. Planta Med 63: 141-145. https://doi.org/10.1055/s-2006-957631
  6. Hyun JS, Oh DS, Lee HD, Kang HB, Lee CW, Cha WS. 2007. Analysis of mineral, amino acid and vitamin contents of fruiting body of Sparassis crispa. J Life Sci 9: 1290-1293. https://doi.org/10.5352/JLS.2007.17.9.1290
  7. Ohno N, Miura N, Nakajima M, Yadomae T. 2000. Antitumor ${\beta}$-1,3-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull 23: 866-872. https://doi.org/10.1248/bpb.23.866
  8. Shon MY, Seo KI, Choi SY, Sung NY, Lee SW, Park SK. 2006. Chemical compounds and biological activity of phellinus baumii. J Korean Soc Food Sci Nutr 35: 524-529. https://doi.org/10.3746/jkfn.2006.35.5.524
  9. Lee SK, Yoo YJ, Kim CS. 1989. Studies on the chemical components in Ganoderma lucidum. Korean J Food Sci Technol 21: 890-894.
  10. Hong JS, Kim YH, Kim MK, Kim YS, Sohn HS. 1989. Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Korean J Food Sci Technol 21: 58-62.
  11. Korea Food & Drug Administration. 2003. Conduct laboratory testing according to specifications and test methods of the food code. In Food Code. Moon Yong Press, Seoul, Korea. p 894-918.
  12. Nam JW. 2004. Studies on short-term mass cultivation and immune substance activity by enzyme treatment of Sparassis crispa. Ministry for Food, Agriculture, Forestry and Fisheries. Gwacheon-si, Gyeonggi-do, Korea.
  13. Ohno NO, Harada T, Masuzawa S, Miura NN, Adachi Y, Nakajima M, Yadomae T. 2001. Antitumor activity and hematopoietic response of a ${\beta}$-glucan extracted from the mushroom Sparassis crispa (Wulf.) Fr. Int J Med Mushrooms 3: 232.
  14. Huo H, Guo X, Hong S, Jiang M, Liu X, Liao K. 2003. Lipid rafts/caveolae are essential for insulin-like growth factor-1 receptor signaling during 3T3-L1 preadipocyte differentiation induction. J Biol Chem 278: 11561-11569. https://doi.org/10.1074/jbc.M211785200
  15. Lee YJ, An MS, Hong KH. 1998. A study on the content of general compounds, amino acid, vitamins, catechins, alkaloids in green, oolong and black tea. J Fd Hyg Safety 13: 377-382.
  16. Kim MU, Kwon OJ, Woo HS, Cho YJ. 2007. Culture condition for biomass of Pleurotus eryngii. J Korean Soc Appl Biol Chem 50: 1-5.
  17. Harada T, Miura N, Adachi Y, Nakajima M, Yadomae T, Ohno N. 2002. Effect of SCG, 1,3-${\beta}$-D-glucan from Sparassis crispa on the hematopoietic response in cyclophosphamide induced leukopenic mice. Biol Pharm Bull 25: 931-939. https://doi.org/10.1248/bpb.25.931
  18. Waters Associates. 1983. Official method of amino acid analysis. In Amino Acid Analysis System of Operators Manual. Waters Associates, Washington, DC, USA. p 37.
  19. Cha WS, Lee HD, Kim JS. 2004. Study on the composition of Pleurotus ferulae fruit body. J Life Sci 14: 205-208. https://doi.org/10.5352/JLS.2004.14.2.205
  20. Green A, Rumberger JM, Stuart CA, Ruhoff MS. 2004. Stimulation of lipolysis by tumor necrosis factor-alpha in 3T3-L1 adipocytes is glucose dependent; implications for long-term regulation of lipolysis. Diabetes 53: 74-81. https://doi.org/10.2337/diabetes.53.1.74
  21. Ahn IS, Do MS, Kim SO, Jung HS, Kim YI, Kim HJ, Park KY. 2006. Antiobesity effect of Kochujang (Korean fermented red pepper paste) extract in 3T3-L1 adipocytes. J Med Food 9: 15-21. https://doi.org/10.1089/jmf.2006.9.15
  22. Horton TJ, Geissler CA. 1996. Post-prandial thermogenesis with ephedrine, caffeine and aspirin in lean, pre-disposed obese and obese women. Int J Obes 20: 91-97.
  23. Lee E. 2003. Effects of powdered pine needle (Pinus densiflora seib et Zucc.) on serum and liver lipid composition and antioxidative capacity in rats fed high oxidized fat. J Korean Soc Food Nutr 32: 926-930. https://doi.org/10.3746/jkfn.2003.32.6.926
  24. Hevener AL, Reichart D, Olefsky J. 2000. Exercise and thiazolidinedione therapy normalize insulin action in Zucker fatty rat. Diabetes 49: 2154-2159. https://doi.org/10.2337/diabetes.49.12.2154

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