KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Activity of the Water Extract of Sargassum fulvellum

참모자반 (Sargassum fulvellum) 물 추출물의 염증 억제 활성

  • Jeong, Da-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences/Pukyong National University) ;
  • Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Jung, Seul-A (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Kim, Hyun-Jee (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Jeong, Hee-Ye (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukoung National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukoung National University)
  • 정다현 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 수산과학연구소) ;
  • 강보경 (부경대학교 식품공학과/식품연구소) ;
  • 정슬아 (부경대학교 식품공학과/식품연구소) ;
  • 김현지 (부경대학교 식품공학과/식품연구소) ;
  • 정희예 (부경대학교 식품공학과/식품연구소) ;
  • 박시우 (부경대학교 식품공학과/식품연구소) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2012.10.31
  • Accepted : 2012.12.11
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The anti-inflammatory effects of Sargassum fulvellum water extracts (SFWE) were investigated using lipopolysaccharide (LPS)-induced inflammatory response in this study. To examine the potential anti-inflammatory properties of SFWE, the NO, TNF-${\alpha}$, IL-6, IL-$1{\beta}$, and cell proliferation were measured. It was confirmed that the NO and TNF-${\alpha}$ secretion were significantly suppressed when SFWE was added to LPS-stimulated RAW 264.7 cells. Moreover, the expression of IL-6 and IL-$1{\beta}$ cytokines was suppressed by SFWE in a dose-dependent manner. Especially, IL-6 inhibition activities were over 50% at 1% of SFWE. The cytotoxicity of SFWE and the proliferation of macrophages was measured by MTT assay. As a result, there was no cytotoxicity in the macrophage proliferation treated with SFWE compared to the control. In conclusion, these results suggested that the SFWE may have significant effects on inflammatory factors and can be a potential anti-inflammatory therapeutic materials.

Keywords

References

  1. Heo, Y. and H. A. Kim (2008) Correlation between skin prick test and enzyme-linked immunoabsorbent assay using serum for identification of subjects positive to major respiratory allergens. J. Env. Hlth. Sci. 24: 369-373.
  2. Park, G. H., J. Y. Lee, D. H. Kim, Y. J. Cho, and B. I. An (2011) Anti-oxidant and antiinflammatory effects of Rosa multiflora root. J. Life Sci. 21: 1120-1126. https://doi.org/10.5352/JLS.2011.21.8.1120
  3. Jew, S. S., O. N. Bae, and J. H. Chung (2003) Anti-inflammatory effects of asiaticoside on inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cell line. J. Toxicol. Pub. Health 19: 33-37.
  4. Ljung, T., S. Lundberg, M. Varsanyi, C. Johansson, P. T. Schmidt, M. Herulf, J. O. Jundberg, and P. M. Hellstrom (2006) Rectal nitric oxide as biomaker in the treatmen of inflammatory bowel disease: responders versus non-responders. World J. Gastroenterol. 12: 3386-3392. https://doi.org/10.3748/wjg.v12.i21.3386
  5. Higuchi, M., N. Hisgahi, H. Taki, and T. Osawa (1990) Cytolytic mechanism of activetaed macrophages. Tumor necrosis factor and L-arginine-dependent mechanism act synergistically as the major cytolytic mechanism of activated macrophages. J. Immunol. 144: 1425-1431.
  6. Guha, M. and N. Mackman (2001) LPS induction of gene expression in human monocyte. Cell Signal. 13: 85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  7. Gracie, J. A., R. J. Forsey, W. L. Chan, A. Cilmour, B. P. Leung, M. R. Greer, K. Kennedy, R. Carter, X. Q. Wei, D. Xu, M. Field, A. Foulis, F. Y. Liew, and I. B. Mclnnes (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J. Clin. Invest. 13: 85-94.
  8. Chen, Y. Y., J. F. Liu, C. M. Chen, P. Y. Chao, and T. J. Chang (2003) A study of antioxidative and antimutagenic effects of Houttuynia cordata thunb using an oxidized frying oil-fed model. J. Nutr. Sci. Vitaminol. 49: 327-333. https://doi.org/10.3177/jnsv.49.327
  9. Lee, K. H., J. O. Nam, and W. H. Yoon (2007) Effect of proteinbound polysaccharide isolated from Acanthopananx senticosus in reducing the toxic effect of cisplatin. Korean J. Pharmacogn. 38: 1-17.
  10. Kawashima, S., M. Hayashi, R. Takii, H. Kimura, H. L. Zhang, A. Nagatsu, J. Sakakibara, K. Murata, Y. Oomoto, and K. Onozaki (1998) Serotonin derivative, N-($\rho$-coumaroyl) serotonin, inhibits the production of TNF-$\alpha$, IL-$1 \alpha$, IL-$1 \beta$ and IL-6 by endotoxinstimulated human blood monocytes. J. Interf. Cytok, Res. 18: 423-428. https://doi.org/10.1089/jir.1998.18.423
  11. Yun, Y. G., G. M, Kim, S. J. Lee, S. H. Ryu, and S. I. Jang (2007) Inhibitory effect of aqueous extract from Lonicera japonica flower on LPS-induced inflammatory mediators in RAW 264.7 macrophages. Korean J. Herbology 22: 117-125.
  12. Lee, H. N., D. Y. Lim, S. S. Lim, J. D. Kim, and J. H. Y. Park (2011) Anti-inflammatory effect of ethanol extract from Eupatorium japonicum. Korean J. Food Sci. Technol. 43: 65-71. https://doi.org/10.9721/KJFST.2011.43.1.065
  13. Yoon, W. J., J. A. Lee, K. N. Kim, J. Y. Kim, and S. Y. Park (2007) In vitro anti-inflammatory activity of the Artemisia fukudo extract in murine macrophage RAW 264.7 cells. Korean J. Food Sci. Technol. 39: 464-469.
  14. Imm, J. Y. and S. J. Kim (2010) Anti-cancer and anti-inflammatory effects of mung bean and soybean extract. Korean J. Food Sci. Technol. 42: 755-761.
  15. Bae, S. J. (2004) Anticarcinogenic effects of Sargassum fulvellum fraction on several human cancer cell lines in vitro. J. Korean Soc. Food Sci. Nutr. 33: 480-486. https://doi.org/10.3746/jkfn.2004.33.3.480
  16. Lee, H. S., H. S. Jung, and H. S. Kuen (2000) Preparation of antibacterial agent from seaweed extract and its antibacterial effect. J. Korean Fish Soc. 33: 32-37.
  17. Lee, H. B., B. W. Choi, J. H. Chun, and B. S. Yu (1996) Extraction of water soluble antioxidants from seaweeds. J. Korean Ind. Eng. Chem. 7: 1069-1077.
  18. Weisz, A., L. Cicatiello, and H. Esumi (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NGmonomethyl- L-arginine. Biochem. J. 316: 209-215. https://doi.org/10.1042/bj3160209
  19. Jirik, F. R., T. J. Podor, T. Hirano, T. Kishimoto, D. J. Loskuroff, D. A. Carson, and M. Lotz (1989) Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J. Immunol. 142: 144-147.
  20. Brian, T. (2002) The effect of fetal acidemia on fetal-placental vascular tone and production of inflammatory cytokines interleukin-6 and tumor necrosis factor-$\alpha$. Am. J. Obstet. Gynenol. 187: 894-897. https://doi.org/10.1067/mob.2002.127455
  21. Charles, N. (2003) Expression of cyclooxygenase-2 in cervical, endometrial and ovarian malignancies. Am. J. Obstet. Gynenol. 188: 1174-1176. https://doi.org/10.1067/mob.2003.284
  22. Sung, J. H., D. H. Choi, D. H. Kim, B. G. Chun, and S. H. Choi (2004) White ginseng saponin upregulated the production of TNF-$\alpha$, IL-$1 \beta$, and NO in primary cultures of mixed glial cells. J. Ginseng Res. 28: 120-126. https://doi.org/10.5142/JGR.2004.28.2.120
  23. Yoon, W. J., Y. M. Ham, S. S. Kim, B. S. Yoo, J. Y. Moon, J. S. Baik, N. H. See, and C. G. Hyun (2009) Suppression of proinflammatory cytokines iNOS and COX-2 expression by brown algae Sargassum micracanthumin RAW 264.7 macrophages. Eurasia. J. Biosix. 3: 130-143.
  24. Jin, D. Q., C. S. Lim, J. Y. Sung, H. G. Choi, I. H. Ha, and J. S. Han (2006) Ulva conglabata, a marine alfa, has neuroprotective and anti-inflammatory effects in murine hippocampal and microglial cells. Neurosci. Lett. 402: 154-158. https://doi.org/10.1016/j.neulet.2006.03.068

Cited by

  1. Comparison Study of Immunomodulatory Activity of Polysaccharide and Ethanol Extracted from Sargassum fulvellum vol.44, pp.11, 2015, https://doi.org/10.3746/jkfn.2015.44.11.1621
  2. 채진목 에탄올 추출물의 항염증 효과 검증 vol.43, pp.1, 2012, https://doi.org/10.15230/scsk.2017.43.1.19
  3. Pharmaceutical and Nutraceutical Potential Applications of Sargassum fulvellum vol.2020, pp.None, 2012, https://doi.org/10.1155/2020/2417410