Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Volatile Flavor Compounds in Commercial Black Garlic Extracts

시판 흑마늘추출액의 휘발성 향기성분

  • Jeon, Seon-Young (Dept. of Food and Nutrition, Changwon National University) ;
  • Baek, Jeong-Hwa (Dept. of Food and Nutrition, Changwon National University) ;
  • Jeong, Eun-Jeong (Dept. of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Dept. of Food and Nutrition, Changwon National University)
  • 전선영 (창원대학교 식품영양학과) ;
  • 백정화 (창원대학교 식품영양학과) ;
  • 정은정 (창원대학교 식품영양학과) ;
  • 차용준 (창원대학교 식품영양학과)
  • Received : 2011.10.14
  • Accepted : 2012.01.12
  • Published : 2012.01.31
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Abstract

Volatile flavor compounds derived from four black garlic extracts purchased in a local market were analyzed for the purpose of quality assessment. A total of 68 compounds was detected in samples using solid phase microextraction (SPME)/GC/MSD, and they were mainly sulfur-containing compounds, including three unknown compounds (21), aldehydes (10), furans (7), alcohols (6), aromatic compounds (7), ketones (4), acids (4), nitrogen-containing compounds (3), esters (2), and miscellaneous compounds (4). 2,6-Dimethyl-4-heptanone having a fruity-sweet odor was the most abundant in all of the samples. Six sulfur-containing compounds including allyl sulfide, 4-methyl-1,2,4-thiazole, 1,3,5-trithiane, unknown I (RI 1564), unknown II (RI 1565), and unknown III (RI 1613) were detected in all of the samples and appeared to contribute to the garlic-like odor. Particularly, three aldehydes (3-methylbutanal, benzaldehyde, phenylacetaldehyde), four furans (furfural, 2-acetylfuran, 5-methyl-2-furfural, furfural alcohol), and others (2,6-dimethylpyrazine, acetic acid) formed through a Maillard reaction during garlic aging were detected in all of the samples, and they contributed to the characteristic burnt, sweet, and sour flavors of black garlic extracts.

흑마늘은 일정기간 마늘의 자체 성분과 효소 등에 의해 숙성되어 진한 흑갈색으로 변한 것으로 추출액과 같은 가공형태로 시중에 많이 유통되고 있으나 관능적 품질평가에 대한 연구는 아직 미흡한 실정이다. 본 연구에서는 국내 흑마늘 제품의 산지별에 따른 흑마늘추출액 4종(A, B, C, D)을 선정하여 관능적 요소 중 가장 중요한 휘발성 향기성분을 분석하여 제품의 품질에 대한 기초자료로 활용하고자 하였다. 시판 흑마늘추출액의 휘발성 향기성분을 분석한 결과, 총 68종으로 함황화합물류 21종, 알데히드류 10종, 퓨란류 7종, 알콜류 6종, 방향족화합물 7종, 케톤류 4종, 산류 4종, 질소화합물 3종, 에스테르류 2종 및 기타 4종이 검출되었다. 흑마늘추출액 모든 시료에서 fruit-sweet향의 2,6-dimethyl-4-heptanone이 높은 함량 검출되었다. 함황화합물에서는 6종, allyl sulfide, 4-methyl-1,2,4-thiazole, 1,3,5-trithiane, unknown I(RI 1564), II(RI 1565), III(R I1613)이 전 시료에서 높은 함량 검출되었으며, 마늘향기성분에 관여할 것으로 사료된다. 또한 Maillard 반응에 의해 형성되어지는 것으로 알려진 3종의 알데히드류(3-methylbutanal, benzaldehyde, phenylacetaldehyde)와 4종의 furan류(furfural, 2-acetylfuran, 5-methyl-2-furfural, furfuryl alcohol), 2,6-dimethyl pyrazine, acetic acid가 모든 시료에서 높은 함량으로 검출되었으며, 이들 향기성분들은 시판 흑마늘추출액의 휘발성성분에 burnt, sweet, sour한 특징적인 냄새에 기여할 것으로 사료되었다.

Keywords

References

  1. Chung DH, Chung SO. 2005. Garlic science. World science, Seoul, Korea. p 9.
  2. Machizuki E, Yamamoto T, Suzuki S, Nakazawa H. 1996. Electrophoretic identification of garlic and garlic products. J AOAC Int 79: 1466-1470.
  3. Rose P, Whiteman M, Moore PK, Zhu YZ. 2005. Bioactive S-alk(en)yl cysteine sulfoxide metabolites in the genus Allium: The chemistry of potential therapeutic agent. Nat Prod Rep 22: 351-368. https://doi.org/10.1039/b417639c
  4. Jang HW, Ka MH, Lee KG. 2008. Antioxidant activity and characterization of volatile extracts of Capsicum annuum L. and Allium spp. Flavour Fragr J 23: 178-184. https://doi.org/10.1002/ffj.1872
  5. Sprnins VL, Barany G, Wattenberg LW. 1988. Effects of organosulfur compounds from garlic and onions on benzo [α]pyrene-induced neoplasia and gluthathione S-transferase activity tn the mouse. Carcinogenesis 9: 131-134. https://doi.org/10.1093/carcin/9.1.131
  6. Shin JH, Choi DJ, Lee SJ, Cha JY, Kim JG, Sung NJ. 2008. Changes of physicochemical components and antioxidant activity of garlic during its processing. J Life Science 18: 1123-1131. https://doi.org/10.5352/JLS.2008.18.8.1123
  7. Shin JH, Lee HJ, Kang MJ, Lee SJ, Sung NJ. 2010. Antioxidant activity of solvent fraction from black garlic. J Korean Soc Food Sci Nutr 39: 933-940. https://doi.org/10.3746/jkfn.2010.39.7.933
  8. Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ, Shin JH. 2008. Physicochemical characteristics of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr 37: 465-471 https://doi.org/10.3746/jkfn.2008.37.4.465
  9. Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ. 2008. Antioxidant activity of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr 37: 965-971. https://doi.org/10.3746/jkfn.2008.37.8.965
  10. You BR, Kim HR, Kim MJ, Kim MR. 2011. Comparison of the quality characteristics and antioxidant activities of the commercial black garlic and lab-prepared fermented and aged black garlic. J Korean Soc Food Sci Nutr 40: 366-371. https://doi.org/10.3746/jkfn.2011.40.3.366
  11. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 69-74.
  12. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrate and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299: 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  13. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014. https://doi.org/10.1021/jf0115589
  14. Cha YJ, Kim H, Park SY, Kim SJ, You YJ. 2000. Identification of irradiation-induced volatile flavor compounds in beef. J Korean Soc Food Sci Nutr 29: 1050-1056.
  15. Jang EK, Seo JH, Lee SP. 2008. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) extract. Korean J Food Technol 40: 443-448.
  16. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphon Elderly Study. Lancet 342: 1007-1011. https://doi.org/10.1016/0140-6736(93)92876-U
  17. Kwon OC, Woo KS, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336.
  18. Shin DB, Seog HM, Kim JH, Lee YC. 1999. Flavor composition of garlic from different area. Korean J Food Sci Technol 31: 293-300.
  19. Jeong JY, Woo KS, Hwang IG, Yoon HS, Lee YR, Jeong HS. 2007. Effects of heat treatment and antioxidant activity of aroma on garlic harvested in different cultivation areas. J Korean Soc Food Sci Nutr 36: 1637-1642. https://doi.org/10.3746/jkfn.2007.36.12.1637
  20. Brondnitz MH, Pascale JV, Derslice LV. 1971. Flavor components of garlic extract. J Agric Food Chem 19: 273-275. https://doi.org/10.1021/jf60174a007
  21. Kim NY. 2010. Effects of processing methods on the distribution of volatile in garlic by SPME. MS Thesis. Seoul National University of Technology, Seoul, Korea. p 31-34.
  22. Sakaguchi M. 1998. Maillard reaction flavor from model system. New Food Industry 30: 42-48.
  23. Espinosa-Mansilla A, Salinas F, Berzas-Nevado JJ. 1992. Differential determination of furfural and hydroxymethylfurfural by derivative spectrophotometry. J AOAC Int 75: 678-684.
  24. Fujimaki M, Kutata T. 1971. Roasted aroma in foods. Gakhak ot Seibutze 9: 85-96.
  25. Lee SH, Woo YH, Kyung KH. 2006. Allyl alcohol found in heated garlic is a potent selective inhibitor of yeasts. J Microbiol Biotechnol 16: 1236-1239.
  26. Lee YJ. 1997. Changes of processing in food flavor. Food Science and Industry 30: 10-25.
  27. Yoon HN. 1996. Sensory characterization of roasted sesame seed oils using gas chromatographic date. Korean J Food Sci Technol 28: 1101-1104.
  28. Flavors & Fragrances. 2008. SAFC supply $solutions^{TM}$. Milwaukee, WI, USA.

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