The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Forsythiae Fructus ethanol extract on inflammatory cytokine production and cellular signaling pathways in mouse macrophages

연교(連翹) 에탄올 추출물의 대식세포의 염증성 사이토카인 합성과 신호전달에 대한 조절

  • Nam, Jung-Bum (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University) ;
  • Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, KyungHee University) ;
  • Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, KyungHee University) ;
  • Sohn, Nak-Won (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University) ;
  • Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
  • 남정범 (경희대학교 동서의학대학원 동서의과학과) ;
  • 이미화 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 손낙원 (경희대학교 동서의학대학원 동서의과학과) ;
  • 강희 (경희대학교 동서의학대학원 동서의과학과)
  • Received : 2011.12.14
  • Accepted : 2012.01.13
  • Published : 2012.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was performed to evaluate the effect of Forsythiae Fructus (FF) ethanol extract on inflammatory cytokine production and its underlying mechanisms in mouse macrophages. Methods : Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with lipopolysaccharide(LPS) or LPS/interferon(IFN)-${\gamma}$ for cytokine measurement and cellular signaling molecule analysis. Results : FF ethanol extract decreased the levels of secreted tumor necrosis factor(TNF)-${\alpha}$ and interleukin(IL)-6 in IFN-${\gamma}$/LPS-stimulated cells in a concentration-dependent manner. FF extract reduced IFN-${\gamma}$/LPS-induced STAT1 phosphorylation and LPS-induced p38 and JNK activation, but not ERK1/2 activity. The extract also inhibited LPS-induced $I{\kappa}B{\alpha}$ degradation through suppression of $I{\kappa}B{\alpha}$ kinase. Conclusions : These results suggest that FF ethanol extract affects the production of TNF-${\alpha}$ and IL-6 through inhibition of activation of STAT-1, $I{\kappa}B{\alpha}$, p38, and JNK.

Keywords

References

  1. Herbology Association of Korea's Colleges of Oriental Medicine. Textbook of Herbology. Younglimsa. 1st ed. 1991;199.
  2. Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol 2008;8:958-969. https://doi.org/10.1038/nri2448
  3. Gasetel M, Kotlyarov A, Kracht M. Targeting innate immunity protein kinase signalling in inflammation. Nat Rev Drug Discuv 2009;8:480-499. https://doi.org/10.1038/nrd2829
  4. Kim SY, Park YK. Ethanol extract of Forsythiae Fructus inhibits the production of inflammtory mediators in LPS-stimulated BV-2 microglia cells. Kor J Herbology 2008;23(3):93-102.
  5. Amber IJ, Hibbs JB Jr, Parker CJ, Johnson BB, Taintor RR, Vavrin Z. Activated macrophages conditioned medium:identification of the soluble factors inducing cytotoxicity and the L-arginine dependent effector mechanism J Leukoc Biol 1991;49:610-620. https://doi.org/10.1002/jlb.49.6.610
  6. Rawlings JS, Rosler KM, Harrison DA. The JAK/STAT signaling pathway. J Cell Sci 2004;117:1281-1283. https://doi.org/10.1242/jcs.00963
  7. D'Acquisto F, May MJ, Ghosh S. Inhibition of Nuclear Factor Kappa B(NF-$\kappa$B):An Emerging Theme in Anti-Inflammatory Therapies. Mol Interv. 2002;2(1):22-35. https://doi.org/10.1124/mi.2.1.22
  8. Kono H, Rock KL. How dying cells alert the immune system to danger. Nature Rev. Immunol. 2008;8:279-289. https://doi.org/10.1038/nri2215
  9. hen CJ, Kono H, Goldenbock D, Reed G, Akira S, Rock KL. Identification of a key pathway required for the sterile inflammatory response triggerrered by dying cells. Nature Med 2007;13:851-856. https://doi.org/10.1038/nm1603
  10. Gordon S. The macrophage:past, present and future. Eur J Immunol 2007;37:S9-S17. https://doi.org/10.1002/eji.200737638
  11. Ramadori G, Damme JV, Rieder H, Buschenfelde KH. Interleukin 6, the third mediator of acute phase reaction, modulates hepatic protein synthesis in human and mouse. Comparison with interleukin $1\beta$ and tumor necrosis factor $-\alpha$. Eur J Immunol 1988;18:1259-1264. https://doi.org/10.1002/eji.1830180817
  12. Aeberli D, Oertle S, Mauron H, Reichenbach S, Jordi B, Villiger PM. Inhibition of the TNF pathway:use of infliximab and etanercept as remission-inducing agents in cases of therapy=resistant chronic inflammatory disorders. Swiss Med Wkly 2002;132:414-422.
  13. Ershler WB, Keller ET. Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty. Annu Rev Med. 200;51:245-270. https://doi.org/10.1146/annurev.med.51.1.245
  14. Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy - from molecular mechanisms th therapeutic benefits. Biochimica et Biophysica Acta 2005;1754:253-262. https://doi.org/10.1016/j.bbapap.2005.08.017
  15. Kou X, Qi S, Dai W, Luo D, Yin Z. Arctigenin inhibits lipopolysaccharide-induced iNOS expression in RAW264.7 cells through suppressing JAK-STAT signal pathway. Int Immunopharmacol. 2011;11(8):1095-1102. https://doi.org/10.1016/j.intimp.2011.03.005
  16. Jung HW, Mahesh R, Lee JG, Lee SH, Kim YS, Park YK. Pinoresinol from the fruits of Forsythia koreana inhibits inflammatory responses in LPS-activated microglia. Neurosci Lett. 2010;480(3):215-20. https://doi.org/10.1016/j.neulet.2010.06.043
  17. Arjumand W, Seth A, Sultana S. Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NF$\kappa$B, TNF-$\alpha$ and caspase-3 expression in wistar rats. Food Chem Toxicol. 2011;49(9):2013-21. https://doi.org/10.1016/j.fct.2011.05.012
  18. Hausmann M, Obermeier F, Paper DH, Balan K, Dunger N, Menzel K, Falk W, Schoelmerich J, Herfarth H, Rogler G. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. Clin Exp Immunol 2007;148(2):373-81. https://doi.org/10.1111/j.1365-2249.2007.03350.x

Cited by

  1. The Suppressive Effect of Th2 Cytokines Expression and the Signal Transduction Mechanism in MC/9 Mast Cells by Forsythiae Fructus Extracts vol.28, pp.3, 2014, https://doi.org/10.7778/jpkm.2014.28.3.031
  2. 국내산 삼채 에탄올 추출물의 항염증 효과 vol.27, pp.6, 2012, https://doi.org/10.6116/kjh.2012.27.6.55