Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) in RAW264.7 cells

RAW264.7 세포주를 통한 복분자 30% 에탄올 추출물의 면역기능 활성증진효과 검토

  • Cho, Jae-Kyung (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Choi, Han-Seok (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Kim, Min-Su (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Kim, Young-Gook (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Kim, Chi-Hong (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Shin, Yong-Cheol (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University) ;
  • Ko, Seong-Gyu (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
  • 조재경 (경희대학교 한의과대학 예방의학교실) ;
  • 최한석 (경희대학교 한의과대학 예방의학교실) ;
  • 김민수 (경희대학교 한의과대학 예방의학교실) ;
  • 김용국 (경희대학교 한의과대학 예방의학교실) ;
  • 김치홍 (경희대학교 한의과대학 예방의학교실) ;
  • 신용철 (경희대학교 한의과대학 예방의학교실) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실)
  • Received : 2012.11.28
  • Accepted : 2012.12.26
  • Published : 2012.12.31
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Abstract

Objective : The object of this study is to confirm the immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) on RAW264.7 mouse macrophage cell line. Methods : RAW264.7 cells were treated with $10-500{\mu}g/mL$ RC for 24 hours. Cell viability was then measured using WST assays. Levels of intracellular NO and ROS were measured by Griess reagent and DCFH-DA staining respectively. Levels of iNOS and COX-2 mRNA was determined by RT-PCR. Secretion levels of IL-$1{\beta}$ and IL-6 cytokines were evaluated by sandwich ELISA assay. Western blot analysis was performed to measure the levels of intracellular molecules related to MAPKs pathways. Results : RC suppressed the growth of RAW264.7 cells. RC increased the production of NO and ROS. RC increased the mRNA and protein levels of COX-2 and iNOS. RC augmented the levels of secreted IL-$1{\beta}$ and IL-6 cytokines. RC increased MAPKs phosphorylation. Conclusion : In summary, our result shows that RC has inflammatory effect increasing the levels of NO, ROS and secreted cytokines and activating MAPKs. Hence, RC seems to have an immune enhancement effect.

Keywords

References

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